1AHE
ASPARTATE AMINOTRANSFERASE HEXAMUTANT
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-09-14 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 85.070, 77.980, 88.860 |
| 格子定数 [度] | 90.00, 118.63, 90.00 |
精密化法
| 残基 | 8.000 - 2.300 |
| R因子 | 0.203 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.270 |
| Data reduction software | MADNES |
| Refinement software | TNT |
Quality characteristics
| Overall | |
| 独立反射数 | 42704 * |
| 完全性 [%] | 88.6 * |
| 冗長性 | 1.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | DTT | 0.5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.90-1.95 (M) | |
| 7 | 1 | reservoir | N-methylmorpholine | 200 (mM) | |
| 8 | 1 | reservoir | PEG400 | 2 (%) |
