1ADU
EARLY E2A DNA-BINDING PROTEIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06-27 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.000, 91.200, 149.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 3.000 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.29600 |
RMSD bond length | 0.006 |
RMSD bond angle | 23.600 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 3.000 | 3.000 * |
Rmerge | 0.168 | 0.496 * |
Total number of observations | 73641 * | |
Number of reflections | 17172 | |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5 (%) | |
2 | 1 | reservoir | ammonium sulfate | 13 (%sat) |