1A27

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 C-TERMINAL DELETION MUTANT COMPLEXED WITH ESTRADIOL AND NADP+

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	130
Detector technology	CCD
Collection date	1996-02
Detector	ESRF
Spacegroup name	C 1 2 1
格子定数 [Å]	111.380, 48.540, 60.770
格子定数 [度]	90.00, 115.13, 90.00

精密化法

残基	10.000 - 1.900
Rwork	0.210
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fdt
結合長の平均二乗偏差(RMSD) [Å]	0.014
結合角の平均二乗偏差(RMSD) [度]	0.030
Data reduction software	XDS
Phasing software	AMoRE
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.000
分解能 [Å] (高)	1.900	1.900
独立反射数	15988
完全性 [%]	70.6	34.5
冗長性	2.83	1.12

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		7.1		PROTEIN WAS CRYSTALLIZED FROM 12-16 % PEG4000, 100 MM HEPES PH 7.1, 0.5 MM ESTRADIOL, 200 MM NACL, 100MM MGCL2, 2.2 MM DECYL-BETA-D-MALTOSIDE; THEN SOAKED IN 30 % PEG 4000, 100 MM HEPES PH 7.1, 100 MM MGCL2, 0.5 MM ESTRADIOL, 1 MM NADP+