1A25
C2 DOMAIN FROM PROTEIN KINASE C (BETA)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 1996-10 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 77.798, 77.798, 140.826 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 2.700 |
| R因子 | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsy |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.2) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 2.900 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| Rmerge_l_obs | 0.136 | 0.130 |
| 独立反射数 | 12297 | |
| <I/σ(I)> | 13 | 8 |
| 完全性 [%] | 98.5 | 95 |
| 冗長性 | 8.9 | 5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 6.4 * | 21 * | PROTEIN WAS CRYSTALLIZED FROM 15% PEG1500, 100 MM MES, PH 6.5 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | PEG1500 | 15 (%(w/v)) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | o-phospho-L-serine | 2 (mM) | |
| 4 | 1 | drop | MES | 100 (mM) |
