1A21
TISSUE FACTOR (TF) FROM RABBIT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-03 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 42.540, 49.010, 65.010 |
| 格子定数 [度] | 96.06, 99.11, 91.14 |
精密化法
| 残基 | 10.000 - 2.350 |
| R因子 | 0.191 |
| Rwork | 0.191 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hft |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 60.000 | 2.400 |
| 分解能 [Å] (高) | 2.350 | 2.350 |
| Rmerge_l_obs | 0.075 | 0.070 * |
| 独立反射数 | 20678 | |
| 完全性 [%] | 96.4 | 90.6 |
| 冗長性 | 3 | 2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | SITTING DROP, 80 UL OF PROTEIN SOLUTION (10 MG/ML PROTEIN IN 50 MM TRIS*HCL,PH:8.0, 50 MM NACL, 75 MM LI2SO4, 12.5 % PEG 3350) EQUILIBRATED AGAINST 30 ML RESERVOIR SOLUTION (50 MM TRIS*HCL, PH:8.0, 50 MM NACL, 150 MM LI2SO4, 25 % PEG3350), vapor diffusion - sitting drop |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | PEG3350 | 12.5 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | 150 (mM) | ||
| 9 | 1 | reservoir | PEG3350 | 25 (%) |
