1A1O
MHC CLASS I MOLECULE B*5301 COMPLEXED WITH PEPTIDE LS6 (KPIVQYDNF) FROM THE MALARIA PARASITE P. FALCIPARUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-09 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 51.300, 83.300, 112.400 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 15.000 - 2.300 |
| R因子 | 0.168 |
| Rwork | 0.168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hsa |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 15.000 |
| 分解能 [Å] (高) | 2.300 |
| Rmerge_l_obs | 0.090 |
| 独立反射数 | 27424 |
| 完全性 [%] | 97.1 |
| 冗長性 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 6.5 | 21 * | 18% PEG8000 0.1M SODIUM CACODYLATE PH6.5 0.2M CALCIUM ACETATE |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | PEG8000 | 18 (%) |
