1U21
transthyretin with tethered inhibitor on one monomer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.654, 85.643, 63.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.630 - 1.690 |
| R-factor | 0.21783 |
| Rwork | 0.217 |
| R-free | 0.23865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bzd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.552 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.630 | 1.760 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 26531 | |
| <I/σ(I)> | 33.2 | 2.3 |
| Completeness [%] | 96.5 | 88.5 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 4000, calcium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
