1GBG
BACILLUS LICHENIFORMIS BETA-GLUCANASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.330, 39.130, 43.880 |
| Unit cell angles | 64.66, 105.86, 110.68 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.165 |
| R-free | 0.23600 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 26.090 * |
| Data reduction software | MOSFLM |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.082 |
| Number of reflections | 17238 * |
| Completeness [%] | 94.6 * |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 44 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 20 (%) | |
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | reservoir | potassium phosphate | 50 (mM) |
