196D
CRYSTAL STRUCTURE OF C-T-C-T-C-G-A-G-A-G: IMPLICATIONS FOR THE STRUCTURE OF THE HOLLIDAY JUNCTION
Experimental procedure
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.230, 24.320, 40.830 |
| Unit cell angles | 90.00, 126.78, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.2 |
| RMSD bond length | 0.037 |
| RMSD bond angle | 0.045 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.056 * |
| Number of reflections | 4946 |
| Completeness [%] | 87.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | standing drop * | 5 * | VAPOR DIFFUSION, SITTING DROP, temperature 278.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CA ACETATE | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 1.34 (mM) | |
| 2 | 1 | drop | calcium acetate | 100-250 (mM) | |
| 3 | 1 | drop | MPD | 15-19 (%(v/v)) |
