196D
CRYSTAL STRUCTURE OF C-T-C-T-C-G-A-G-A-G: IMPLICATIONS FOR THE STRUCTURE OF THE HOLLIDAY JUNCTION
Experimental procedure
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 62.230, 24.320, 40.830 |
| 格子定数 [度] | 90.00, 126.78, 90.00 |
精密化法
| 残基 | 8.000 - 1.700 |
| R因子 | 0.2 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.037 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.045 |
| Refinement software | NUCLSQ |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 1.700 |
| Rmerge_l_obs | 0.056 * |
| 独立反射数 | 4946 |
| 完全性 [%] | 87.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | standing drop * | 5 * | VAPOR DIFFUSION, SITTING DROP, temperature 278.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CA ACETATE | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | DNA duplex | 1.34 (mM) | |
| 2 | 1 | drop | calcium acetate | 100-250 (mM) | |
| 3 | 1 | drop | MPD | 15-19 (%(v/v)) |
