159D
SIDE BY SIDE BINDING OF TWO DISTAMYCIN A DRUGS IN THE MINOR GROOVE OF AN ALTERNATING B-DNA DUPLEX
Experimental procedure
Source type | ROTATING ANODE |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 27.930, 27.930, 57.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.15 |
Rwork | 0.150 |
RMSD bond length | 0.011 |
RMSD bond angle | 3.500 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 16.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.018 * |
Total number of observations | 10245 * |
Number of reflections | 2423 |
Completeness [%] | 94.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | single strand concentration |
2 | 1 | drop | sodium cacodylate | 20 (mM) | |
3 | 1 | drop | distamycin A hydrochloride | 1 (mM) | |
4 | 1 | drop | magnesium chloride | 20 (mM) | |
5 | 1 | drop | MPD | 2.5 (%(v/v)) | |
6 | 1 | reservoir | 60 (%) |