103M

SPERM WHALE MYOGLOBIN H64A N-BUTYL ISOCYANIDE AT PH 9.0

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	RIGAKU
Spacegroup name	P 6
格子定数 [Å]	91.630, 91.630, 46.014
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	5.000 - 2.070
R因子	0.158
Rwork	0.158
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SPERM WHALE MYOGLOBIN 0M D122N (DEOXY)
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.500
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	6.000	2.080
分解能 [Å] (高)	2.070	2.070
Rmerge_l_obs	0.072	0.294
独立反射数	13413
完全性 [%]	98.4	99.5
冗長性	5.17	5.24

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		9		3.0 M AMMONIUM SULFATE, 20 MM TRIS, 1MM EDTA, PH 9.0