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- PDB-5jpq: Cryo-EM structure of the 90S pre-ribosome -

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Basic information

Entry
Database: PDB / ID: 5jpq
TitleCryo-EM structure of the 90S pre-ribosome
Components
  • (WD40 domain proteins) x 2
  • 18S ribosomal RNA
  • Bms1
  • Emg1
  • Imp3
  • KRR1 small subunit processome component
  • Kre33
  • Nop1
  • Pre mRNA splicing protein
  • Putative U3 small nucleolar ribonucleoprotein
  • Rcl1
  • Snu13NHP2L1
  • U3 RNA
  • U3 small nucleolar RNA-associated protein 21
  • UTP-A oligomerization domain
  • UTP-B oligomerisation domain
  • UTP10
  • UTP6
  • Utp24
  • Utp30
  • eS1
  • eS24
  • eS28
  • eS4
  • eS6
  • eS7
  • eS8
  • rrp9
  • uS11
  • uS15
  • uS17
  • uS4
  • uS7
  • uS8
  • uS9
KeywordsRIBOSOME / nuclear RNP
Function / homology
Function and homology information


tRNA acetylation / rRNA small subunit pseudouridine methyltransferase Nep1 / Pwp2p-containing subcomplex of 90S preribosome / histone H2AQ104 methyltransferase activity / nuclear microtubule / rRNA (pseudouridine) methyltransferase activity / regulation of rRNA processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA modification / box C/D sno(s)RNA 3'-end processing ...tRNA acetylation / rRNA small subunit pseudouridine methyltransferase Nep1 / Pwp2p-containing subcomplex of 90S preribosome / histone H2AQ104 methyltransferase activity / nuclear microtubule / rRNA (pseudouridine) methyltransferase activity / regulation of rRNA processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA modification / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / box C/D methylation guide snoRNP complex / rRNA base methylation / rRNA primary transcript binding / ribonuclease P activity / tRNA 5'-leader removal / N-acetyltransferase activity / rRNA methylation / U3 snoRNA binding / : / tRNA processing / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / enzyme activator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / RNA nuclease activity / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA endonuclease activity / Transferases; Transferring one-carbon groups; Methyltransferases / nuclear periphery / small-subunit processome / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / rRNA processing / ribosomal small subunit assembly / cytosolic small ribosomal subunit / ribosome biogenesis / small ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / GTPase activity / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / : / Archaeal Nop5/56-rel, N-terminal domain / : / : / : / KRR1 small subunit processome component, second KH domain / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain ...: / : / Archaeal Nop5/56-rel, N-terminal domain / : / : / : / KRR1 small subunit processome component, second KH domain / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / TmcA/NAT10/Kre33 / Helicase / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / GNAT acetyltransferase 2 / Ribosomal RNA assembly KRR1 / rRNA-processing protein Fcf1, PIN domain / Ribosomal RNA-processing protein Rrp9-like / rRNA-processing protein Fcf1/Utp23 / Fcf1 / BP28, C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosome biogenesis protein Bms1, N-terminal / U3 small nucleolar RNA-associated protein 10 / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / RNA 3'-terminal phosphate cyclase signature. / BP28CT (NUC211) domain / Small-subunit processome, Utp21 / RNA 3'-terminal phosphate cyclase type 2 / Utp21 specific WD40 associated putative domain / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Nucleolar protein Nop56/Nop58 / Krr1, KH1 domain / Krr1 KH1 domain / rRNA 2'-O-methyltransferase fibrillarin-like / Fibrillarin, conserved site / Fibrillarin / Fibrillarin signature. / Fibrillarin / U3 snoRNP protein/Ribosome production factor 1 / Large family of predicted nucleotide-binding domains / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / AARP2CN (NUC121) domain / Bms1-type guanine nucleotide-binding (G) domain profile. / AARP2CN (NUC121) domain / Protein of unknown function (DUF663) / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / PIN domain / Ribosomal protein L7Ae, archaea / Brix domain / Brix domain / Brix domain profile. / Brix / Anaphase-promoting complex subunit 4, WD40 domain / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Anaphase-promoting complex subunit 4 WD40 domain / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / K Homology domain, type 1 superfamily / PIN-like domain superfamily / Quinoprotein alcohol dehydrogenase-like superfamily / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S3Ae, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein S7e / Ribosomal protein S8e
Similarity search - Domain/homology
RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / C/D box methylation guide ribonucleoprotein complex aNOP56 subunit / 40S ribosomal protein S6 / RNA cytidine acetyltransferase / KRR1 small subunit processome component / U3 small nucleolar RNA-associated protein 10 / Uncharacterized protein ...RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / C/D box methylation guide ribonucleoprotein complex aNOP56 subunit / 40S ribosomal protein S6 / RNA cytidine acetyltransferase / KRR1 small subunit processome component / U3 small nucleolar RNA-associated protein 10 / Uncharacterized protein / Uncharacterized protein / PIN domain-containing protein / U3 small nucleolar ribonucleoprotein protein IMP4 / 40S ribosomal protein S24 / 40S ribosomal protein S7 / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein eL8 / Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / RNA 3'-terminal phosphate cyclase-like protein / Ribosome biogenesis protein BMS1 / 40S ribosomal protein S8 / 40S ribosomal protein S11, putative / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS28A
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus)
Saccharomyces cerevisiae (brewer's yeast)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7.3 Å
AuthorsTurk, M. / Cheng, J. / Berninghausen, O. / Kornprobst, M. / Flemming, D. / Kos-Braun, I.C. / Kos, M. / Thoms, M. / Hurt, E. / Beckmann, R.
CitationJournal: Cell / Year: 2016
Title: Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.
Authors: Markus Kornprobst / Martin Turk / Nikola Kellner / Jingdong Cheng / Dirk Flemming / Isabelle Koš-Braun / Martin Koš / Matthias Thoms / Otto Berninghausen / Roland Beckmann / Ed Hurt /
Abstract: The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that ...The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 β-propellers and large α-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.
History
DepositionMay 4, 2016Deposition site: RCSB / Processing site: PDBE
Revision 1.0Jul 27, 2016Provider: repository / Type: Initial release
Revision 1.1Aug 3, 2016Group: Other
Revision 1.2Jul 5, 2017Group: Structure summary / Category: entity / Item: _entity.pdbx_description
Revision 1.3Oct 24, 2018Group: Advisory / Data collection / Derived calculations
Category: em_image_scans / ndb_struct_na_base_pair ...em_image_scans / ndb_struct_na_base_pair / ndb_struct_na_base_pair_step / pdbx_unobs_or_zero_occ_atoms / pdbx_validate_close_contact / struct_conn
Revision 1.4Nov 21, 2018Group: Advisory / Data collection / Derived calculations
Category: pdbx_unobs_or_zero_occ_atoms / pdbx_validate_close_contact / struct_conn
Revision 1.5Jan 23, 2019Group: Advisory / Data collection / Derived calculations
Category: pdbx_unobs_or_zero_occ_atoms / pdbx_validate_close_contact ...pdbx_unobs_or_zero_occ_atoms / pdbx_validate_close_contact / struct_conn / struct_conn_type
Revision 1.6Mar 11, 2020Group: Source and taxonomy / Category: entity_src_nat / Item: _entity_src_nat.pdbx_ncbi_taxonomy_id

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Structure visualization

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Assembly

Deposited unit
A: WD40 domain proteins
B: WD40 domain proteins
C: WD40 domain proteins
D: WD40 domain proteins
E: WD40 domain proteins
F: WD40 domain proteins
G: UTP10
H: UTP-A oligomerization domain
I: U3 small nucleolar RNA-associated protein 21
J: WD40 domain proteins
K: WD40 domain proteins
L: WD40 domain proteins
M: WD40 domain proteins
N: WD40 domain proteins
O: WD40 domain proteins
P: WD40 domain proteins
Q: UTP6
R: UTP-B oligomerisation domain
S: Pre mRNA splicing protein
T: Pre mRNA splicing protein
U: Snu13
V: Snu13
W: Nop1
X: Nop1
Y: rrp9
Z: Rcl1
a: Bms1
b: Imp3
c: Putative U3 small nucleolar ribonucleoprotein
d: Utp24
e: Emg1
f: Emg1
g: KRR1 small subunit processome component
h: KRR1 small subunit processome component
i: Kre33
j: Kre33
k: Utp30
l: WD40 domain proteins
m: WD40 domain proteins
n: WD40 domain proteins
o: eS1
p: eS4
q: uS7
r: eS6
s: eS7
t: eS8
u: uS4
v: uS15
w: uS11
x: uS9
y: uS17
z: uS8
0: eS24
1: eS28
2: 18S ribosomal RNA
3: U3 RNA


Theoretical massNumber of molelcules
Total (without water)3,984,52656
Polymers3,984,52656
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 34 types, 54 molecules ABCDEFJKLNPlnGHIMOmQRSTUVWXYZa...

#1: Protein
WD40 domain proteins


Mass: 109801.734 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#2: Protein UTP10


Mass: 198508.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5L1
#3: Protein UTP-A oligomerization domain


Mass: 78313.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#4: Protein U3 small nucleolar RNA-associated protein 21 / U3 snoRNA-associated protein 21 / U three protein 21


Mass: 104927.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
References: UniProt: Q06078
#5: Protein WD40 domain proteins


Mass: 74058.758 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#6: Protein UTP6 /


Mass: 38825.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#7: Protein UTP-B oligomerisation domain


Mass: 47676.848 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#8: Protein Pre mRNA splicing protein


Mass: 46765.828 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: A0A0E3MJI1*PLUS
#9: Protein Snu13 / NHP2L1


Mass: 14074.316 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P55858*PLUS
#10: Protein Nop1


Mass: 26455.375 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P58032*PLUS
#11: Protein rrp9 /


Mass: 65146.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q06506*PLUS
#12: Protein Rcl1 /


Mass: 40220.559 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q08096*PLUS
#13: Protein Bms1


Mass: 135792.281 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q08965*PLUS
#14: Protein Imp3 /


Mass: 21802.396 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SDL4
#15: Protein Putative U3 small nucleolar ribonucleoprotein


Mass: 34216.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum / References: UniProt: G0SE90
#16: Protein Utp24


Mass: 21103.770 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SE30
#17: Protein Emg1 /


Mass: 27936.461 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q06287*PLUS
#18: Protein KRR1 small subunit processome component / / KRR-R motif-containing protein 1


Mass: 37500.777 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S3V7
#19: Protein Kre33


Mass: 119795.125 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S273
#20: Protein Utp30


Mass: 43700.527 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S7X0
#21: Protein eS1


Mass: 29709.248 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q23DE3*PLUS
#22: Protein eS4


Mass: 29512.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P0C233*PLUS
#23: Protein uS7


Mass: 25072.600 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P26783*PLUS
#24: Protein eS6


Mass: 32614.617 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: A4VD76*PLUS
#25: Protein eS7


Mass: 23231.061 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: I7MD19*PLUS
#26: Protein eS8


Mass: 24250.346 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q22AV0*PLUS
#27: Protein uS4


Mass: 22487.893 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: O13516*PLUS
#28: Protein uS15


Mass: 17059.945 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P05756*PLUS
#29: Protein uS11


Mass: 14562.655 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P06367*PLUS
#30: Protein uS9


Mass: 15877.490 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P0CX51*PLUS
#31: Protein uS17


Mass: 18142.395 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q22B78*PLUS
#32: Protein uS8


Mass: 14650.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P0C0W1*PLUS
#33: Protein eS24


Mass: 17128.072 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: I7MAL3*PLUS
#34: Protein eS28


Mass: 7605.847 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: Q3E7X9*PLUS

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RNA chain , 2 types, 2 molecules 23

#35: RNA chain 18S ribosomal RNA /


Mass: 579761.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#36: RNA chain U3 RNA


Mass: 87969.609 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 90s pre-ribosome / Type: RIBOSOME / Entity ID: all / Source: NATURAL
Source (natural)Organism: Chaetomium thermophilum (fungus)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 16 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

CTF correctionType: NONE
3D reconstructionResolution: 7.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 43000 / Symmetry type: POINT

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