[English] 日本語
Yorodumi- PDB-6f3k: Combined solid-state NMR, solution-state NMR and EM data for stru... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6f3k | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Combined solid-state NMR, solution-state NMR and EM data for structure determination of the tetrahedral aminopeptidase TET2 from P. horikoshii | ||||||||||||
Components | Tetrahedral aminopeptidase | ||||||||||||
Keywords | PEPTIDE BINDING PROTEIN / peptidase / protein quality control / oligomer / aminopeptidase | ||||||||||||
Function / homology | Peptidase M42, domain 2 / M42 glutamyl aminopeptidase / Peptidase M42 / Hydrolases; Acting on peptide bonds (peptidases); Aminopeptidases / aminopeptidase activity / metallopeptidase activity / proteolysis / metal ion binding / Tetrahedral aminopeptidase Function and homology information | ||||||||||||
Biological species | Pyrococcus horikoshii (archaea) | ||||||||||||
Method | ELECTRON MICROSCOPY / SOLUTION NMR / SOLID-STATE NMR / single particle reconstruction / cryo EM / Resolution: 4.1 Å | ||||||||||||
Authors | Gauto, D.F. / Estrozi, L.F. / Schwieters, C.D. / Effantin, G. / Macek, P. / Sounier, R. / Kerfah, R. / Sivertsen, A.C. / Colletier, J.P. / Boisbouvier, J. ...Gauto, D.F. / Estrozi, L.F. / Schwieters, C.D. / Effantin, G. / Macek, P. / Sounier, R. / Kerfah, R. / Sivertsen, A.C. / Colletier, J.P. / Boisbouvier, J. / Schoehn, G. / Favier, A. / Schanda, P. | ||||||||||||
Funding support | France, 3items
| ||||||||||||
Citation | Journal: Nat Commun / Year: 2019 Title: Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex. Authors: Diego F Gauto / Leandro F Estrozi / Charles D Schwieters / Gregory Effantin / Pavel Macek / Remy Sounier / Astrid C Sivertsen / Elena Schmidt / Rime Kerfah / Guillaume Mas / Jacques-Philippe ...Authors: Diego F Gauto / Leandro F Estrozi / Charles D Schwieters / Gregory Effantin / Pavel Macek / Remy Sounier / Astrid C Sivertsen / Elena Schmidt / Rime Kerfah / Guillaume Mas / Jacques-Philippe Colletier / Peter Güntert / Adrien Favier / Guy Schoehn / Paul Schanda / Jerome Boisbouvier / Abstract: Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM and NMR spectroscopy both provide structural information, but currently cryo-EM does not routinely ...Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM and NMR spectroscopy both provide structural information, but currently cryo-EM does not routinely give access to atomic-level structural data, and, generally, NMR structure determination is restricted to small (<30 kDa) proteins. We introduce an integrated structure determination approach that simultaneously uses NMR and EM data to overcome the limits of each of these methods. The approach enables structure determination of the 468 kDa large dodecameric aminopeptidase TET2 to a precision and accuracy below 1 Å by combining secondary-structure information obtained from near-complete magic-angle-spinning NMR assignments of the 39 kDa-large subunits, distance restraints from backbone amides and ILV methyl groups, and a 4.1 Å resolution EM map. The resulting structure exceeds current standards of NMR and EM structure determination in terms of molecular weight and precision. Importantly, the approach is successful even in cases where only medium-resolution cryo-EM data are available. | ||||||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6f3k.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6f3k.ent.gz | 917.8 KB | Display | PDB format |
PDBx/mmJSON format | 6f3k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f3/6f3k ftp://data.pdbj.org/pub/pdb/validation_reports/f3/6f3k | HTTPS FTP |
---|
-Related structure data
Related structure data | 4179MC 6r8nC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| x 12|||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 39071.027 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (archaea) Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 Gene: frvX, PH1527 / Production host: Escherichia coli (E. coli) References: UniProt: O59196, Hydrolases; Acting on peptide bonds (peptidases); Aminopeptidases |
---|---|
#2: Chemical |
-Experimental details
-Experiment
Experiment |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR experiment |
|
-Sample preparation
Component | Name: tetrahedral amino-peptidase from P. horikoshii / Type: COMPLEX / Details: H78C/H248C mutant / Entity ID: #1 / Source: RECOMBINANT | ||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Molecular weight | Value: 0.49 MDa / Experimental value: NO | ||||||||||||||||||||||||||||||||||||||||||||
Source (natural) | Organism: Pyrococcus horikoshii (archaea) | ||||||||||||||||||||||||||||||||||||||||||||
Source (recombinant) | Organism: Escherichia coli (E. coli) | ||||||||||||||||||||||||||||||||||||||||||||
Buffer solution | pH: 7.4 | ||||||||||||||||||||||||||||||||||||||||||||
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: This sample was monodisperse. | ||||||||||||||||||||||||||||||||||||||||||||
Specimen support | Grid material: COPPER / Grid mesh size: 400 divisions/in. / Grid type: Quantifoil R1.2/1.3 | ||||||||||||||||||||||||||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 293 K / Details: Blotting time 2s, force 1, drain time 0. | ||||||||||||||||||||||||||||||||||||||||||||
Details |
| ||||||||||||||||||||||||||||||||||||||||||||
Sample |
| ||||||||||||||||||||||||||||||||||||||||||||
Sample conditions |
|
-Data collection
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Microscopy | Model: FEI POLARA 300 | ||||||||||||||||||
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM | ||||||||||||||||||
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 20000 X / Calibrated magnification: 25773 X / Calibrated defocus min: 800 nm / Calibrated defocus max: 3500 nm / Cs: 2 mm / Alignment procedure: COMA FREE | ||||||||||||||||||
Specimen holder | Cryogen: NITROGEN Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER | ||||||||||||||||||
Image recording | Average exposure time: 4 sec. / Electron dose: 40 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of real images: 90 | ||||||||||||||||||
Image scans | Movie frames/image: 40 | ||||||||||||||||||
NMR spectrometer |
|
-Processing
EM software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 30407 | ||||||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: T (tetrahedral) | ||||||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 27130 / Algorithm: FOURIER SPACE / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: OTHER | ||||||||||||||||||||||||||||||||||||||||
NMR software |
| ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 10 |