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- PDB-6zon: SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome compl... -

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Basic information

Entry
Database: PDB / ID: 6zon
TitleSARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 1
Components
  • (40S ribosomal protein ...) x 31
  • (Eukaryotic translation initiation factor 3 subunit ...Eukaryotic initiation factor 3) x 11
  • 18S ribosomal RNA
  • 60S ribosomal protein L41
  • Host translation inhibitor Nsp1
  • Receptor of activated protein C kinase 1
  • Ubiquitin-40S ribosomal protein S27a
  • Unknown factor
KeywordsVIRAL PROTEIN / Translational Inhibition / SARS-CoV-2 / Immune Evasion / Human Ribosome
Function / homology
Function and homology information


positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / IRES-dependent viral translational initiation / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex ...positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / IRES-dependent viral translational initiation / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / cytoplasmic translational initiation / mRNA cap binding / formation of cytoplasmic translation initiation complex / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / protein tyrosine kinase inhibitor activity / eukaryotic 48S preinitiation complex / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of respiratory burst involved in inflammatory response / positive regulation of gastrulation / IRE1-RACK1-PP2A complex / nucleolus organization / metal-dependent deubiquitinase activity / response to extracellular stimulus / positive regulation of endodeoxyribonuclease activity / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / negative regulation of DNA repair / negative regulation of RNA splicing / laminin receptor activity / oxidized purine DNA binding / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / supercoiled DNA binding / neural crest cell differentiation / NF-kappaB complex / rRNA modification in the nucleus and cytosol / negative regulation of phagocytosis / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / positive regulation of ubiquitin-protein transferase activity / Formation of the ternary complex, and subsequently, the 43S complex / regulation of translational initiation / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / pigmentation / protein kinase A binding / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / phagocytic cup / positive regulation of mitochondrial depolarization / negative regulation of Wnt signaling pathway / positive regulation of T cell receptor signaling pathway / positive regulation of activated T cell proliferation / fibroblast growth factor binding / regulation of cell division / SARS-CoV-1 modulates host translation machinery / iron-sulfur cluster binding / Protein hydroxylation / TOR signaling / protein deubiquitination / BH3 domain binding / mTORC1-mediated signalling / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Peptide chain elongation / Selenocysteine synthesis / monocyte chemotaxis / cysteine-type endopeptidase activator activity involved in apoptotic process / Formation of a pool of free 40S subunits / ribosomal small subunit export from nucleus / positive regulation of cyclic-nucleotide phosphodiesterase activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / translation regulator activity / SRP-dependent cotranslational protein targeting to membrane / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / negative regulation of respiratory burst involved in inflammatory response / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / gastrulation / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / spindle assembly / regulation of translational fidelity / MDM2/MDM4 family protein binding / laminin binding / Protein methylation / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / rough endoplasmic reticulum
Similarity search - Function
Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain ...Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor eIF2A / Ubiquitin-like protein FUBI / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / Anaphase-promoting complex subunit 4, WD40 domain / 40S ribosomal protein SA C-terminus / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Anaphase-promoting complex subunit 4 WD40 domain / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / : / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S19e, conserved site / : / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S19A/S15e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e
Similarity search - Domain/homology
: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 ...: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Replicase polyprotein 1ab / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å
AuthorsThoms, M. / Buschauer, R. / Ameismeier, M. / Denk, T. / Kratzat, H. / Mackens-Kiani, T. / Cheng, J. / Berninghausen, O. / Becker, T. / Beckmann, R.
Funding support Germany, 3items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB/TRR-174 Germany
German Research Foundation (DFG)BE1814/15-1 Germany
German Research Foundation (DFG)BE1814/1-1 Germany
CitationJournal: Science / Year: 2020
Title: Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2.
Authors: Matthias Thoms / Robert Buschauer / Michael Ameismeier / Lennart Koepke / Timo Denk / Maximilian Hirschenberger / Hanna Kratzat / Manuel Hayn / Timur Mackens-Kiani / Jingdong Cheng / Jan H ...Authors: Matthias Thoms / Robert Buschauer / Michael Ameismeier / Lennart Koepke / Timo Denk / Maximilian Hirschenberger / Hanna Kratzat / Manuel Hayn / Timur Mackens-Kiani / Jingdong Cheng / Jan H Straub / Christina M Stürzel / Thomas Fröhlich / Otto Berninghausen / Thomas Becker / Frank Kirchhoff / Konstantin M J Sparrer / Roland Beckmann /
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current coronavirus disease 2019 (COVID-19) pandemic. A major virulence factor of SARS-CoVs is the ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current coronavirus disease 2019 (COVID-19) pandemic. A major virulence factor of SARS-CoVs is the nonstructural protein 1 (Nsp1), which suppresses host gene expression by ribosome association. Here, we show that Nsp1 from SARS-CoV-2 binds to the 40 ribosomal subunit, resulting in shutdown of messenger RNA (mRNA) translation both in vitro and in cells. Structural analysis by cryo-electron microscopy of in vitro-reconstituted Nsp1-40 and various native Nsp1-40 and -80 complexes revealed that the Nsp1 C terminus binds to and obstructs the mRNA entry tunnel. Thereby, Nsp1 effectively blocks retinoic acid-inducible gene I-dependent innate immune responses that would otherwise facilitate clearance of the infection. Thus, the structural characterization of the inhibitory mechanism of Nsp1 may aid structure-based drug design against SARS-CoV-2.
History
DepositionJul 7, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 29, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 16, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Structure visualization

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Assembly

Deposited unit
a: 40S ribosomal protein SA
p: 40S ribosomal protein S3a
d: 40S ribosomal protein S2
Q: 40S ribosomal protein S26
q: 40S ribosomal protein S4, X isoform
W: 60S ribosomal protein L41
r: 40S ribosomal protein S6
s: 40S ribosomal protein S7
t: 40S ribosomal protein S8
c: 40S ribosomal protein S9
n: 40S ribosomal protein S11
m: 40S ribosomal protein S13
i: 40S ribosomal protein S14
y: 40S ribosomal protein S21
f: 40S ribosomal protein S15a
j: 40S ribosomal protein S23
z: 40S ribosomal protein S24
R: 40S ribosomal protein S27
T: 40S ribosomal protein S30
2: 18S ribosomal RNA
w: 40S ribosomal protein S17
g: 40S ribosomal protein S16
b: 40S ribosomal protein S3
e: 40S ribosomal protein S5
u: 40S ribosomal protein S10
v: 40S ribosomal protein S12
o: 40S ribosomal protein S15
k: 40S ribosomal protein S18
x: 40S ribosomal protein S19
h: 40S ribosomal protein S20
P: 40S ribosomal protein S25
S: 40S ribosomal protein S28
l: 40S ribosomal protein S29
U: Ubiquitin-40S ribosomal protein S27a
V: Receptor of activated protein C kinase 1
I: Eukaryotic translation initiation factor 3 subunit I
B: Eukaryotic translation initiation factor 3 subunit B
A: Eukaryotic translation initiation factor 3 subunit A,Eukaryotic translation initiation factor 3 subunit A
C: Eukaryotic translation initiation factor 3 subunit C
E: Eukaryotic translation initiation factor 3 subunit E
F: Eukaryotic translation initiation factor 3 subunit F
H: Eukaryotic translation initiation factor 3 subunit H
K: Eukaryotic translation initiation factor 3 subunit K
L: Eukaryotic translation initiation factor 3 subunit L
M: Eukaryotic translation initiation factor 3 subunit M
D: Eukaryotic translation initiation factor 3 subunit D
Y: Unknown factor
J: Host translation inhibitor Nsp1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,894,01751
Polymers1,893,82148
Non-polymers1963
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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40S ribosomal protein ... , 31 types, 31 molecules apdQqrstcnmiyfjzRTwgbeuvokxhPSl

#1: Protein 40S ribosomal protein SA / / 37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin ...37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin receptor / 67LR / Colon carcinoma laminin-binding protein / Laminin receptor 1 / LamR / Laminin-binding protein precursor p40 / LBP/p40 / Multidrug resistance-associated protein MGr1-Ag / NEM/1CHD4 / Small ribosomal subunit protein uS2


Mass: 32883.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P08865
#2: Protein 40S ribosomal protein S3a / / Small ribosomal subunit protein eS1 / v-fos transformation effector protein / Fte-1


Mass: 30002.061 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P61247
#3: Protein 40S ribosomal protein S2 / / 40S ribosomal protein S4 / Protein LLRep3 / Small ribosomal subunit protein uS5


Mass: 31376.516 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P15880
#4: Protein 40S ribosomal protein S26 / / Small ribosomal subunit protein eS26


Mass: 13047.532 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62854
#5: Protein 40S ribosomal protein S4, X isoform / Ribosome / SCR10 / Single copy abundant mRNA protein / Small ribosomal subunit protein eS4


Mass: 29654.869 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62701
#7: Protein 40S ribosomal protein S6 / / Phosphoprotein NP33 / Small ribosomal subunit protein eS6


Mass: 28751.906 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62753
#8: Protein 40S ribosomal protein S7 / / Small ribosomal subunit protein eS7


Mass: 22168.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62081
#9: Protein 40S ribosomal protein S8 / / Small ribosomal subunit protein eS8


Mass: 24263.387 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62241
#10: Protein 40S ribosomal protein S9 / / Small ribosomal subunit protein uS4


Mass: 22641.564 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P46781
#11: Protein 40S ribosomal protein S11 / / Small ribosomal subunit protein uS17


Mass: 18468.826 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62280
#12: Protein 40S ribosomal protein S13 / / Small ribosomal subunit protein uS15


Mass: 17259.389 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62277
#13: Protein 40S ribosomal protein S14 / / Small ribosomal subunit protein uS11


Mass: 16302.772 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62263
#14: Protein 40S ribosomal protein S21 / / Small ribosomal subunit protein eS21


Mass: 9124.389 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P63220
#15: Protein 40S ribosomal protein S15a / / Small ribosomal subunit protein uS8


Mass: 14865.555 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62244
#16: Protein 40S ribosomal protein S23 / / Small ribosomal subunit protein uS12


Mass: 15844.666 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62266
#17: Protein 40S ribosomal protein S24 / / Small ribosomal subunit protein eS24


Mass: 15463.333 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62847
#18: Protein 40S ribosomal protein S27 / / Metallopan-stimulin 1 / MPS-1 / Small ribosomal subunit protein eS27


Mass: 9480.186 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P42677
#19: Protein 40S ribosomal protein S30 / / Small ribosomal subunit protein eS30


Mass: 6668.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62861
#21: Protein 40S ribosomal protein S17 / / Small ribosomal subunit protein eS17


Mass: 15578.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P08708
#22: Protein 40S ribosomal protein S16 / / Small ribosomal subunit protein uS9


Mass: 16477.377 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62249
#23: Protein 40S ribosomal protein S3 / / Small ribosomal subunit protein uS3


Mass: 26729.369 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria)
References: UniProt: P23396, DNA-(apurinic or apyrimidinic site) lyase
#24: Protein 40S ribosomal protein S5 / / Small ribosomal subunit protein uS7


Mass: 22913.453 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P46782
#25: Protein 40S ribosomal protein S10 / / Small ribosomal subunit protein eS10


Mass: 18933.846 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P46783
#26: Protein 40S ribosomal protein S12 / / Small ribosomal subunit protein eS12


Mass: 14538.987 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P25398
#27: Protein 40S ribosomal protein S15 / / RIG protein / Small ribosomal subunit protein uS19


Mass: 17076.207 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62841
#28: Protein 40S ribosomal protein S18 / / Ke-3 / Ke3 / Small ribosomal subunit protein uS13


Mass: 17759.777 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62269
#29: Protein 40S ribosomal protein S19 / / Small ribosomal subunit protein eS19


Mass: 16091.562 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P39019
#30: Protein 40S ribosomal protein S20 / / Small ribosomal subunit protein uS10


Mass: 13398.763 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P60866
#31: Protein 40S ribosomal protein S25 / / Small ribosomal subunit protein eS25


Mass: 13776.224 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62851
#32: Protein 40S ribosomal protein S28 / / Small ribosomal subunit protein eS28


Mass: 7855.052 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62857
#33: Protein 40S ribosomal protein S29 / / Small ribosomal subunit protein uS14


Mass: 6690.821 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62273

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Protein , 4 types, 4 molecules UVYJ

#34: Protein Ubiquitin-40S ribosomal protein S27a / Ubiquitin carboxyl extension protein 80


Mass: 18004.041 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62979
#35: Protein Receptor of activated protein C kinase 1 / Cell proliferation-inducing gene 21 protein / Guanine nucleotide-binding protein subunit beta-2- ...Cell proliferation-inducing gene 21 protein / Guanine nucleotide-binding protein subunit beta-2-like 1 / Guanine nucleotide-binding protein subunit beta-like protein 12.3 / Human lung cancer oncogene 7 protein / HLC-7 / Receptor for activated C kinase / Small ribosomal subunit protein RACK1


Mass: 35115.652 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P63244
#47: Protein Unknown factor


Mass: 6656.196 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria)
#48: Protein Host translation inhibitor Nsp1 / pp1ab / ORF1ab polyprotein / Host translation inhibitor Nsp1


Mass: 19801.287 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Description: sample / Production host: Escherichia coli K-12 (bacteria)
References: UniProt: P0DTD1, ubiquitinyl hydrolase 1, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases, SARS coronavirus main proteinase, RNA-directed RNA polymerase, DNA ...References: UniProt: P0DTD1, ubiquitinyl hydrolase 1, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases, SARS coronavirus main proteinase, RNA-directed RNA polymerase, DNA helicase, RNA helicase, Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters, Hydrolases; Acting on ester bonds, Transferases; Transferring one-carbon groups; Methyltransferases

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Eukaryotic translation initiation factor 3 subunit ... , 11 types, 11 molecules IBACEFHKLMD

#36: Protein Eukaryotic translation initiation factor 3 subunit I / Eukaryotic initiation factor 3 / eIF3i / Eukaryotic translation initiation factor 3 subunit 2 / TGF-beta receptor-interacting ...eIF3i / Eukaryotic translation initiation factor 3 subunit 2 / TGF-beta receptor-interacting protein 1 / TRIP-1 / eIF-3-beta / eIF3 p36


Mass: 36543.773 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q13347
#37: Protein Eukaryotic translation initiation factor 3 subunit B / Eukaryotic initiation factor 3 / eIF3b / Eukaryotic translation initiation factor 3 subunit 9 / Prt1 homolog / hPrt1 / eIF-3-eta / ...eIF3b / Eukaryotic translation initiation factor 3 subunit 9 / Prt1 homolog / hPrt1 / eIF-3-eta / eIF3 p110 / eIF3 p116


Mass: 92593.414 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P55884
#38: Protein Eukaryotic translation initiation factor 3 subunit A,Eukaryotic translation initiation factor 3 subunit A / Eukaryotic initiation factor 3 / eIF3a / Eukaryotic translation initiation factor 3 subunit 10 / eIF-3-theta / eIF3 p167 / eIF3 p180 ...eIF3a / Eukaryotic translation initiation factor 3 subunit 10 / eIF-3-theta / eIF3 p167 / eIF3 p180 / eIF3 p185


Mass: 79249.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1, 602 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q14152
#39: Protein Eukaryotic translation initiation factor 3 subunit C / Eukaryotic initiation factor 3 / eIF3c / Eukaryotic translation initiation factor 3 subunit 8 / eIF3 p110


Mass: 105503.945 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q99613
#40: Protein Eukaryotic translation initiation factor 3 subunit E / Eukaryotic initiation factor 3 / eIF3e / Eukaryotic translation initiation factor 3 subunit 6 / Viral integration site protein INT-6 ...eIF3e / Eukaryotic translation initiation factor 3 subunit 6 / Viral integration site protein INT-6 homolog / eIF-3 p48


Mass: 52281.633 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P60228
#41: Protein Eukaryotic translation initiation factor 3 subunit F / Eukaryotic initiation factor 3 / eIF3f / Deubiquitinating enzyme eIF3f / Eukaryotic translation initiation factor 3 subunit 5 / eIF- ...eIF3f / Deubiquitinating enzyme eIF3f / Eukaryotic translation initiation factor 3 subunit 5 / eIF-3-epsilon / eIF3 p47


Mass: 37593.645 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: O00303, ubiquitinyl hydrolase 1
#42: Protein Eukaryotic translation initiation factor 3 subunit H / Eukaryotic initiation factor 3 / eIF3h / Eukaryotic translation initiation factor 3 subunit 3 / eIF-3-gamma / eIF3 p40 subunit


Mass: 39979.277 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: O15372
#43: Protein Eukaryotic translation initiation factor 3 subunit K / Eukaryotic initiation factor 3 / eIF3k / Eukaryotic translation initiation factor 3 subunit 12 / Muscle-specific gene M9 protein / ...eIF3k / Eukaryotic translation initiation factor 3 subunit 12 / Muscle-specific gene M9 protein / PLAC-24 / eIF-3 p25 / eIF-3 p28


Mass: 25083.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q9UBQ5
#44: Protein Eukaryotic translation initiation factor 3 subunit L / Eukaryotic initiation factor 3 / eIF3l / Eukaryotic translation initiation factor 3 subunit 6-interacting protein / Eukaryotic ...eIF3l / Eukaryotic translation initiation factor 3 subunit 6-interacting protein / Eukaryotic translation initiation factor 3 subunit E-interacting protein


Mass: 66803.734 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q9Y262
#45: Protein Eukaryotic translation initiation factor 3 subunit M / Eukaryotic initiation factor 3 / eIF3m / Fetal lung protein B5 / hFL-B5 / PCI domain-containing protein 1


Mass: 42555.832 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q7L2H7
#46: Protein Eukaryotic translation initiation factor 3 subunit D / Eukaryotic initiation factor 3 / eIF3d / Eukaryotic translation initiation factor 3 subunit 7 / eIF-3-zeta / eIF3 p66


Mass: 64060.758 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: O15371

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Protein/peptide / RNA chain / Non-polymers , 3 types, 5 molecules W2

#20: RNA chain 18S ribosomal RNA /


Mass: 602432.625 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: GenBank: 337376
#49: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Zn
#6: Protein/peptide 60S ribosomal protein L41 / / HG12 / Large ribosomal subunit protein eL41


Mass: 3473.451 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: 1 / Source: (gene. exp.) Homo sapiens (human) / Description: sample / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: P62945

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeEntity IDParent-IDSource
1SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 1COMPLEX#1-#480MULTIPLE SOURCES
2Human 43S preinitiation ribosome complexRIBOSOME#1-#471RECOMBINANT
3SARS-CoV-2 Nsp1COMPLEX#481RECOMBINANT
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
12Homo sapiens (human)9606
23Severe acute respiratory syndrome coronavirus 22697049
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-ID
12Escherichia coli K-12 (bacteria)83333
23Escherichia coli K-12 (bacteria)83333
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 44.8 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 13928 / Symmetry type: POINT

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