+Open data
-Basic information
Entry | Database: PDB / ID: 6jlq | |||||||||
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Title | Crystal structure of human USP46-WDR48-WDR20 complex | |||||||||
Components |
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Keywords | HYDROLASE / Deubiquitinase / DUB / USP46 | |||||||||
Function / homology | Function and homology information regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / adult feeding behavior / righting reflex / behavioral response to ethanol / deubiquitinase activator activity / skeletal system morphogenesis / skin development / regulation of synaptic transmission, GABAergic / positive regulation of double-strand break repair via homologous recombination ...regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / adult feeding behavior / righting reflex / behavioral response to ethanol / deubiquitinase activator activity / skeletal system morphogenesis / skin development / regulation of synaptic transmission, GABAergic / positive regulation of double-strand break repair via homologous recombination / seminiferous tubule development / protein deubiquitination / single fertilization / homeostasis of number of cells / behavioral fear response / embryonic organ development / ubiquitin binding / positive regulation of epithelial cell proliferation / Recognition of DNA damage by PCNA-containing replication complex / Fanconi Anemia Pathway / double-strand break repair via homologous recombination / positive regulation of receptor signaling pathway via JAK-STAT / multicellular organism growth / late endosome / single-stranded DNA binding / double-stranded DNA binding / spermatogenesis / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / lysosome / Ub-specific processing proteases / intracellular membrane-bounded organelle / DNA damage response / proteolysis / DNA binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) Bos mutus (wild yak) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.101 Å | |||||||||
Authors | Zhu, H. / Zhang, T. / Ding, J. | |||||||||
Funding support | China, 2items
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Citation | Journal: Cell Discov / Year: 2019 Title: Structural insights into the activation of USP46 by WDR48 and WDR20. Authors: Zhu, H. / Zhang, T. / Wang, F. / Yang, J. / Ding, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6jlq.cif.gz | 255.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6jlq.ent.gz | 196.7 KB | Display | PDB format |
PDBx/mmJSON format | 6jlq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jl/6jlq ftp://data.pdbj.org/pub/pdb/validation_reports/jl/6jlq | HTTPS FTP |
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-Related structure data
Related structure data | 5k1cS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 41262.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: USP46 / Production host: Escherichia coli (E. coli) / References: UniProt: P62068, ubiquitinyl hydrolase 1 |
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-WD repeat-containing protein ... , 2 types, 2 molecules BC
#2: Protein | Mass: 69730.641 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: WDR48 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q8TAF3 |
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#3: Protein | Mass: 48560.879 Da / Num. of mol.: 1 Fragment: UNP residues 1-318,UNP residues 106-137,UNP residues 535-595 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Bos mutus (wild yak) Gene: WDR20, M91_05678 / Production host: Escherichia coli (E. coli) References: UniProt: Q8TBZ3, UniProt: B3KQX8, UniProt: L8I535 |
-Non-polymers , 3 types, 10 molecules
#4: Chemical | ChemComp-ZN / | ||
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#5: Chemical | ChemComp-GOL / #6: Chemical | ChemComp-PO4 / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.78 Å3/Da / Density % sol: 74.27 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 1.0M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic, pH 7.2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.9791 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 14, 2016 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.1→50 Å / Num. obs: 56304 / % possible obs: 99 % / Redundancy: 11 % / Biso Wilson estimate: 88.06 Å2 / Rmerge(I) obs: 0.141 / Rpim(I) all: 0.041 / Rrim(I) all: 0.148 / Χ2: 2.519 / Net I/σ(I): 9.9 / Num. measured all: 616763 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5K1C Resolution: 3.101→43.986 Å / SU ML: 0.47 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 28.63
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 183.11 Å2 / Biso mean: 95.0071 Å2 / Biso min: 30 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.101→43.986 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 20
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