+Open data
-Basic information
Entry | Database: PDB / ID: 6akk | ||||||
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Title | Crystal structure of the second Coiled-coil domain of SIKE1 | ||||||
Components | Suppressor of IKBKE 1 | ||||||
Keywords | PROTEIN BINDING / homotetramer / Coiled-coil | ||||||
Function / homology | Function and homology information TRAF6 mediated IRF7 activation / small GTPase binding / SARS-CoV-1 activates/modulates innate immune responses / TRAF3-dependent IRF activation pathway / protein kinase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.5 Å | ||||||
Authors | Zhou, L. / Chen, M. / Zhou, Z.C. | ||||||
Citation | Journal: Cell Discov / Year: 2019 Title: Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling. Authors: Tang, Y. / Chen, M. / Zhou, L. / Ma, J. / Li, Y. / Zhang, H. / Shi, Z. / Xu, Q. / Zhang, X. / Gao, Z. / Zhao, Y. / Cheng, Y. / Jiao, S. / Zhou, Z. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6akk.cif.gz | 55.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6akk.ent.gz | 40.3 KB | Display | PDB format |
PDBx/mmJSON format | 6akk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ak/6akk ftp://data.pdbj.org/pub/pdb/validation_reports/ak/6akk | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 6426.421 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SIKE1, SIKE / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / Variant (production host): CodonPlus / References: UniProt: Q9BRV8 #2: Chemical | ChemComp-GOL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.48 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop Details: 1.0 M (NH4)2HPO4 (ammonium dibasic phosphate); 0.1 M acetate pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97915 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: May 27, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 17636 / % possible obs: 97.6 % / Redundancy: 13.4 % / Rmerge(I) obs: 0.091 / Rpim(I) all: 0.026 / Rrim(I) all: 0.095 / Net I/σ(I): 32.5 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 14.1 % / Rmerge(I) obs: 0.582 / Mean I/σ(I) obs: 5.1 / Num. unique obs: 873 / CC1/2: 0.994 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.5→35.598 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 29.21 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→35.598 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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