+Open data
-Basic information
Entry | Database: PDB / ID: 5to5 | ||||||
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Title | Structure of the TPR oligomerization domain | ||||||
Components | Nucleoprotein TPR | ||||||
Keywords | CELL CYCLE / TPR oligomerization domain / Receptor tyrosine kinase / MET / Oncogenic Fusion kinases | ||||||
Function / homology | Function and homology information regulation of mitotic sister chromatid separation / negative regulation of RNA export from nucleus / RNA import into nucleus / mRNA export from nucleus in response to heat stress / cellular response to interferon-alpha / positive regulation of mitotic cell cycle spindle assembly checkpoint / nuclear pore organization / Nuclear Pore Complex (NPC) Disassembly / nuclear inclusion body / nuclear pore nuclear basket ...regulation of mitotic sister chromatid separation / negative regulation of RNA export from nucleus / RNA import into nucleus / mRNA export from nucleus in response to heat stress / cellular response to interferon-alpha / positive regulation of mitotic cell cycle spindle assembly checkpoint / nuclear pore organization / Nuclear Pore Complex (NPC) Disassembly / nuclear inclusion body / nuclear pore nuclear basket / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / positive regulation of intracellular protein transport / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / NS1 Mediated Effects on Host Pathways / cytoplasmic dynein complex / SUMOylation of SUMOylation proteins / response to epidermal growth factor / regulation of mitotic spindle assembly / Transport of Mature mRNA Derived from an Intronless Transcript / RNA export from nucleus / structural constituent of nuclear pore / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / SUMOylation of RNA binding proteins / Transport of Mature mRNA derived from an Intron-Containing Transcript / NEP/NS2 Interacts with the Cellular Export Machinery / tRNA processing in the nucleus / mitogen-activated protein kinase binding / nucleocytoplasmic transport / Viral Messenger RNA Synthesis / dynein complex binding / mitotic spindle assembly checkpoint signaling / SUMOylation of ubiquitinylation proteins / positive regulation of heterochromatin formation / Vpr-mediated nuclear import of PICs / SUMOylation of DNA replication proteins / Signaling by ALK fusions and activated point mutants / Regulation of HSF1-mediated heat shock response / mRNA export from nucleus / SUMOylation of DNA damage response and repair proteins / nuclear pore / negative regulation of translational initiation / heat shock protein binding / nuclear periphery / SUMOylation of chromatin organization proteins / tubulin binding / positive regulation of protein export from nucleus / HCMV Late Events / Transcriptional regulation by small RNAs / mitotic spindle / ISG15 antiviral mechanism / kinetochore / HCMV Early Events / positive regulation of protein import into nucleus / protein import into nucleus / regulation of protein localization / cellular response to heat / nuclear envelope / snRNP Assembly / nuclear membrane / cell division / mRNA binding / chromatin binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / RNA binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Pal, K. / Xu, Q. / Zhou, X.E. / Melcher, K. / Xu, H.E. | ||||||
Citation | Journal: Structure / Year: 2017 Title: Structural Basis of TPR-Mediated Oligomerization and Activation of Oncogenic Fusion Kinases. Authors: Pal, K. / Bandyopadhyay, A. / Zhou, X.E. / Xu, Q. / Marciano, D.P. / Brunzelle, J.S. / Yerrum, S. / Griffin, P.R. / Vande Woude, G. / Melcher, K. / Xu, H.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5to5.cif.gz | 71.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5to5.ent.gz | 53.7 KB | Display | PDB format |
PDBx/mmJSON format | 5to5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/to/5to5 ftp://data.pdbj.org/pub/pdb/validation_reports/to/5to5 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 16747.922 Da / Num. of mol.: 2 / Fragment: UNP residues 2-142 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TPR / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P12270 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.07 % |
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Crystal grow | Temperature: 295 K / Method: evaporation / pH: 6.25 / Details: 16% PEG1.5K, 0.1M Bistris (6.25) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1.07812 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Mar 16, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07812 Å / Relative weight: 1 |
Reflection | Resolution: 2.18→21.85 Å / Num. obs: 17251 / % possible obs: 96 % / Redundancy: 5.7 % / Rmerge(I) obs: 0.079 / Net I/σ(I): 15.8 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→21.411 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.94 / Phase error: 29.96
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Solvent computation | Shrinkage radii: 1 Å / VDW probe radii: 1.1 Å | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 125.41 Å2 / Biso mean: 37.5525 Å2 / Biso min: 18.01 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.5→21.411 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 4
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