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- PDB-5mq7: Structure of AaLS-13 -

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Basic information

Entry
Database: PDB / ID: 5mq7
TitleStructure of AaLS-13
Components6,7-dimethyl-8-ribityllumazine synthaseLumazine synthase
KeywordsTRANSFERASE / cryo-EM / porous protein cage / icosahedral symmetry / engineered lumazine synthase
Function / homology
Function and homology information


6,7-dimethyl-8-ribityllumazine synthase / 6,7-dimethyl-8-ribityllumazine synthase activity / riboflavin synthase complex / riboflavin biosynthetic process / cytosol
Similarity search - Function
Lumazine synthase / Lumazine/riboflavin synthase / Lumazine/riboflavin synthase superfamily / 6,7-dimethyl-8-ribityllumazine synthase
Similarity search - Domain/homology
6,7-dimethyl-8-ribityllumazine synthase
Similarity search - Component
Biological speciesAquifex aeolicus (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 5.2 Å
AuthorsSasaki, E. / Boehringer, D. / Leibundgut, M. / Ban, N. / Hilvert, D.
Funding support1items
OrganizationGrant numberCountry
European Research CouncilERC-dG-2012-321295
CitationJournal: Nat Commun / Year: 2017
Title: Structure and assembly of scalable porous protein cages.
Authors: Eita Sasaki / Daniel Böhringer / Michiel van de Waterbeemd / Marc Leibundgut / Reinhard Zschoche / Albert J R Heck / Nenad Ban / Donald Hilvert /
Abstract: Proteins that self-assemble into regular shell-like polyhedra are useful, both in nature and in the laboratory, as molecular containers. Here we describe cryo-electron microscopy (EM) structures of ...Proteins that self-assemble into regular shell-like polyhedra are useful, both in nature and in the laboratory, as molecular containers. Here we describe cryo-electron microscopy (EM) structures of two versatile encapsulation systems that exploit engineered electrostatic interactions for cargo loading. We show that increasing the number of negative charges on the lumenal surface of lumazine synthase, a protein that naturally assembles into a ∼1-MDa dodecahedron composed of 12 pentamers, induces stepwise expansion of the native protein shell, giving rise to thermostable ∼3-MDa and ∼6-MDa assemblies containing 180 and 360 subunits, respectively. Remarkably, these expanded particles assume unprecedented tetrahedrally and icosahedrally symmetric structures constructed entirely from pentameric units. Large keyhole-shaped pores in the shell, not present in the wild-type capsid, enable diffusion-limited encapsulation of complementarily charged guests. The structures of these supercharged assemblies demonstrate how programmed electrostatic effects can be effectively harnessed to tailor the architecture and properties of protein cages.
History
DepositionDec 20, 2016Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 22, 2017Provider: repository / Type: Initial release

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Structure visualization

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Assembly

Deposited unit
0A: 6,7-dimethyl-8-ribityllumazine synthase
0B: 6,7-dimethyl-8-ribityllumazine synthase
0C: 6,7-dimethyl-8-ribityllumazine synthase
0D: 6,7-dimethyl-8-ribityllumazine synthase
0E: 6,7-dimethyl-8-ribityllumazine synthase
0X: 6,7-dimethyl-8-ribityllumazine synthase
1A: 6,7-dimethyl-8-ribityllumazine synthase
1B: 6,7-dimethyl-8-ribityllumazine synthase
1C: 6,7-dimethyl-8-ribityllumazine synthase
1D: 6,7-dimethyl-8-ribityllumazine synthase
1E: 6,7-dimethyl-8-ribityllumazine synthase
1X: 6,7-dimethyl-8-ribityllumazine synthase
2A: 6,7-dimethyl-8-ribityllumazine synthase
2B: 6,7-dimethyl-8-ribityllumazine synthase
2C: 6,7-dimethyl-8-ribityllumazine synthase
2D: 6,7-dimethyl-8-ribityllumazine synthase
2E: 6,7-dimethyl-8-ribityllumazine synthase
2X: 6,7-dimethyl-8-ribityllumazine synthase
3A: 6,7-dimethyl-8-ribityllumazine synthase
3B: 6,7-dimethyl-8-ribityllumazine synthase
3C: 6,7-dimethyl-8-ribityllumazine synthase
3D: 6,7-dimethyl-8-ribityllumazine synthase
3E: 6,7-dimethyl-8-ribityllumazine synthase
3X: 6,7-dimethyl-8-ribityllumazine synthase
4A: 6,7-dimethyl-8-ribityllumazine synthase
4B: 6,7-dimethyl-8-ribityllumazine synthase
4C: 6,7-dimethyl-8-ribityllumazine synthase
4D: 6,7-dimethyl-8-ribityllumazine synthase
4E: 6,7-dimethyl-8-ribityllumazine synthase
4X: 6,7-dimethyl-8-ribityllumazine synthase
5A: 6,7-dimethyl-8-ribityllumazine synthase
5B: 6,7-dimethyl-8-ribityllumazine synthase
5C: 6,7-dimethyl-8-ribityllumazine synthase
5D: 6,7-dimethyl-8-ribityllumazine synthase
5E: 6,7-dimethyl-8-ribityllumazine synthase
5X: 6,7-dimethyl-8-ribityllumazine synthase
6A: 6,7-dimethyl-8-ribityllumazine synthase
6B: 6,7-dimethyl-8-ribityllumazine synthase
6C: 6,7-dimethyl-8-ribityllumazine synthase
6D: 6,7-dimethyl-8-ribityllumazine synthase
6E: 6,7-dimethyl-8-ribityllumazine synthase
6X: 6,7-dimethyl-8-ribityllumazine synthase
7A: 6,7-dimethyl-8-ribityllumazine synthase
7B: 6,7-dimethyl-8-ribityllumazine synthase
7C: 6,7-dimethyl-8-ribityllumazine synthase
7D: 6,7-dimethyl-8-ribityllumazine synthase
7E: 6,7-dimethyl-8-ribityllumazine synthase
7X: 6,7-dimethyl-8-ribityllumazine synthase
8A: 6,7-dimethyl-8-ribityllumazine synthase
8B: 6,7-dimethyl-8-ribityllumazine synthase
8C: 6,7-dimethyl-8-ribityllumazine synthase
8D: 6,7-dimethyl-8-ribityllumazine synthase
8E: 6,7-dimethyl-8-ribityllumazine synthase
8X: 6,7-dimethyl-8-ribityllumazine synthase
9A: 6,7-dimethyl-8-ribityllumazine synthase
9B: 6,7-dimethyl-8-ribityllumazine synthase
9C: 6,7-dimethyl-8-ribityllumazine synthase
9D: 6,7-dimethyl-8-ribityllumazine synthase
9E: 6,7-dimethyl-8-ribityllumazine synthase
9X: 6,7-dimethyl-8-ribityllumazine synthase
AA: 6,7-dimethyl-8-ribityllumazine synthase
AB: 6,7-dimethyl-8-ribityllumazine synthase
AC: 6,7-dimethyl-8-ribityllumazine synthase
AD: 6,7-dimethyl-8-ribityllumazine synthase
AE: 6,7-dimethyl-8-ribityllumazine synthase
AX: 6,7-dimethyl-8-ribityllumazine synthase
BA: 6,7-dimethyl-8-ribityllumazine synthase
BB: 6,7-dimethyl-8-ribityllumazine synthase
BC: 6,7-dimethyl-8-ribityllumazine synthase
BD: 6,7-dimethyl-8-ribityllumazine synthase
BE: 6,7-dimethyl-8-ribityllumazine synthase
BX: 6,7-dimethyl-8-ribityllumazine synthase
CA: 6,7-dimethyl-8-ribityllumazine synthase
CB: 6,7-dimethyl-8-ribityllumazine synthase
CC: 6,7-dimethyl-8-ribityllumazine synthase
CD: 6,7-dimethyl-8-ribityllumazine synthase
CE: 6,7-dimethyl-8-ribityllumazine synthase
CX: 6,7-dimethyl-8-ribityllumazine synthase
DA: 6,7-dimethyl-8-ribityllumazine synthase
DB: 6,7-dimethyl-8-ribityllumazine synthase
DC: 6,7-dimethyl-8-ribityllumazine synthase
DD: 6,7-dimethyl-8-ribityllumazine synthase
DE: 6,7-dimethyl-8-ribityllumazine synthase
DX: 6,7-dimethyl-8-ribityllumazine synthase
EA: 6,7-dimethyl-8-ribityllumazine synthase
EB: 6,7-dimethyl-8-ribityllumazine synthase
EC: 6,7-dimethyl-8-ribityllumazine synthase
ED: 6,7-dimethyl-8-ribityllumazine synthase
EE: 6,7-dimethyl-8-ribityllumazine synthase
EX: 6,7-dimethyl-8-ribityllumazine synthase
FA: 6,7-dimethyl-8-ribityllumazine synthase
FB: 6,7-dimethyl-8-ribityllumazine synthase
FC: 6,7-dimethyl-8-ribityllumazine synthase
FD: 6,7-dimethyl-8-ribityllumazine synthase
FE: 6,7-dimethyl-8-ribityllumazine synthase
FX: 6,7-dimethyl-8-ribityllumazine synthase
GA: 6,7-dimethyl-8-ribityllumazine synthase
GB: 6,7-dimethyl-8-ribityllumazine synthase
GC: 6,7-dimethyl-8-ribityllumazine synthase
GD: 6,7-dimethyl-8-ribityllumazine synthase
GE: 6,7-dimethyl-8-ribityllumazine synthase
GX: 6,7-dimethyl-8-ribityllumazine synthase
HA: 6,7-dimethyl-8-ribityllumazine synthase
HB: 6,7-dimethyl-8-ribityllumazine synthase
HC: 6,7-dimethyl-8-ribityllumazine synthase
HD: 6,7-dimethyl-8-ribityllumazine synthase
HE: 6,7-dimethyl-8-ribityllumazine synthase
HX: 6,7-dimethyl-8-ribityllumazine synthase
IA: 6,7-dimethyl-8-ribityllumazine synthase
IB: 6,7-dimethyl-8-ribityllumazine synthase
IC: 6,7-dimethyl-8-ribityllumazine synthase
ID: 6,7-dimethyl-8-ribityllumazine synthase
IE: 6,7-dimethyl-8-ribityllumazine synthase
IX: 6,7-dimethyl-8-ribityllumazine synthase
JA: 6,7-dimethyl-8-ribityllumazine synthase
JB: 6,7-dimethyl-8-ribityllumazine synthase
JC: 6,7-dimethyl-8-ribityllumazine synthase
JD: 6,7-dimethyl-8-ribityllumazine synthase
JE: 6,7-dimethyl-8-ribityllumazine synthase
JX: 6,7-dimethyl-8-ribityllumazine synthase
KA: 6,7-dimethyl-8-ribityllumazine synthase
KB: 6,7-dimethyl-8-ribityllumazine synthase
KC: 6,7-dimethyl-8-ribityllumazine synthase
KD: 6,7-dimethyl-8-ribityllumazine synthase
KE: 6,7-dimethyl-8-ribityllumazine synthase
KX: 6,7-dimethyl-8-ribityllumazine synthase
LA: 6,7-dimethyl-8-ribityllumazine synthase
LB: 6,7-dimethyl-8-ribityllumazine synthase
LC: 6,7-dimethyl-8-ribityllumazine synthase
LD: 6,7-dimethyl-8-ribityllumazine synthase
LE: 6,7-dimethyl-8-ribityllumazine synthase
LX: 6,7-dimethyl-8-ribityllumazine synthase
MA: 6,7-dimethyl-8-ribityllumazine synthase
MB: 6,7-dimethyl-8-ribityllumazine synthase
MC: 6,7-dimethyl-8-ribityllumazine synthase
MD: 6,7-dimethyl-8-ribityllumazine synthase
ME: 6,7-dimethyl-8-ribityllumazine synthase
MX: 6,7-dimethyl-8-ribityllumazine synthase
NA: 6,7-dimethyl-8-ribityllumazine synthase
NB: 6,7-dimethyl-8-ribityllumazine synthase
NC: 6,7-dimethyl-8-ribityllumazine synthase
ND: 6,7-dimethyl-8-ribityllumazine synthase
NE: 6,7-dimethyl-8-ribityllumazine synthase
NX: 6,7-dimethyl-8-ribityllumazine synthase
OA: 6,7-dimethyl-8-ribityllumazine synthase
OB: 6,7-dimethyl-8-ribityllumazine synthase
OC: 6,7-dimethyl-8-ribityllumazine synthase
OD: 6,7-dimethyl-8-ribityllumazine synthase
OE: 6,7-dimethyl-8-ribityllumazine synthase
OX: 6,7-dimethyl-8-ribityllumazine synthase
PA: 6,7-dimethyl-8-ribityllumazine synthase
PB: 6,7-dimethyl-8-ribityllumazine synthase
PC: 6,7-dimethyl-8-ribityllumazine synthase
PD: 6,7-dimethyl-8-ribityllumazine synthase
PE: 6,7-dimethyl-8-ribityllumazine synthase
PX: 6,7-dimethyl-8-ribityllumazine synthase
QA: 6,7-dimethyl-8-ribityllumazine synthase
QB: 6,7-dimethyl-8-ribityllumazine synthase
QC: 6,7-dimethyl-8-ribityllumazine synthase
QD: 6,7-dimethyl-8-ribityllumazine synthase
QE: 6,7-dimethyl-8-ribityllumazine synthase
QX: 6,7-dimethyl-8-ribityllumazine synthase
RA: 6,7-dimethyl-8-ribityllumazine synthase
RB: 6,7-dimethyl-8-ribityllumazine synthase
RC: 6,7-dimethyl-8-ribityllumazine synthase
RD: 6,7-dimethyl-8-ribityllumazine synthase
RE: 6,7-dimethyl-8-ribityllumazine synthase
RX: 6,7-dimethyl-8-ribityllumazine synthase
SA: 6,7-dimethyl-8-ribityllumazine synthase
SB: 6,7-dimethyl-8-ribityllumazine synthase
SC: 6,7-dimethyl-8-ribityllumazine synthase
SD: 6,7-dimethyl-8-ribityllumazine synthase
SE: 6,7-dimethyl-8-ribityllumazine synthase
SX: 6,7-dimethyl-8-ribityllumazine synthase
TA: 6,7-dimethyl-8-ribityllumazine synthase
TB: 6,7-dimethyl-8-ribityllumazine synthase
TC: 6,7-dimethyl-8-ribityllumazine synthase
TD: 6,7-dimethyl-8-ribityllumazine synthase
TE: 6,7-dimethyl-8-ribityllumazine synthase
TX: 6,7-dimethyl-8-ribityllumazine synthase
UA: 6,7-dimethyl-8-ribityllumazine synthase
UB: 6,7-dimethyl-8-ribityllumazine synthase
UC: 6,7-dimethyl-8-ribityllumazine synthase
UD: 6,7-dimethyl-8-ribityllumazine synthase
UE: 6,7-dimethyl-8-ribityllumazine synthase
UX: 6,7-dimethyl-8-ribityllumazine synthase
VA: 6,7-dimethyl-8-ribityllumazine synthase
VB: 6,7-dimethyl-8-ribityllumazine synthase
VC: 6,7-dimethyl-8-ribityllumazine synthase
VD: 6,7-dimethyl-8-ribityllumazine synthase
VE: 6,7-dimethyl-8-ribityllumazine synthase
VX: 6,7-dimethyl-8-ribityllumazine synthase
WA: 6,7-dimethyl-8-ribityllumazine synthase
WB: 6,7-dimethyl-8-ribityllumazine synthase
WC: 6,7-dimethyl-8-ribityllumazine synthase
WD: 6,7-dimethyl-8-ribityllumazine synthase
WE: 6,7-dimethyl-8-ribityllumazine synthase
WX: 6,7-dimethyl-8-ribityllumazine synthase
XA: 6,7-dimethyl-8-ribityllumazine synthase
XB: 6,7-dimethyl-8-ribityllumazine synthase
XC: 6,7-dimethyl-8-ribityllumazine synthase
XD: 6,7-dimethyl-8-ribityllumazine synthase
XE: 6,7-dimethyl-8-ribityllumazine synthase
XX: 6,7-dimethyl-8-ribityllumazine synthase
YA: 6,7-dimethyl-8-ribityllumazine synthase
YB: 6,7-dimethyl-8-ribityllumazine synthase
YC: 6,7-dimethyl-8-ribityllumazine synthase
YD: 6,7-dimethyl-8-ribityllumazine synthase
YE: 6,7-dimethyl-8-ribityllumazine synthase
YX: 6,7-dimethyl-8-ribityllumazine synthase
ZA: 6,7-dimethyl-8-ribityllumazine synthase
ZB: 6,7-dimethyl-8-ribityllumazine synthase
ZC: 6,7-dimethyl-8-ribityllumazine synthase
ZD: 6,7-dimethyl-8-ribityllumazine synthase
ZE: 6,7-dimethyl-8-ribityllumazine synthase
ZX: 6,7-dimethyl-8-ribityllumazine synthase
aA: 6,7-dimethyl-8-ribityllumazine synthase
aB: 6,7-dimethyl-8-ribityllumazine synthase
aC: 6,7-dimethyl-8-ribityllumazine synthase
aD: 6,7-dimethyl-8-ribityllumazine synthase
aE: 6,7-dimethyl-8-ribityllumazine synthase
aX: 6,7-dimethyl-8-ribityllumazine synthase
bA: 6,7-dimethyl-8-ribityllumazine synthase
bB: 6,7-dimethyl-8-ribityllumazine synthase
bC: 6,7-dimethyl-8-ribityllumazine synthase
bD: 6,7-dimethyl-8-ribityllumazine synthase
bE: 6,7-dimethyl-8-ribityllumazine synthase
bX: 6,7-dimethyl-8-ribityllumazine synthase
cA: 6,7-dimethyl-8-ribityllumazine synthase
cB: 6,7-dimethyl-8-ribityllumazine synthase
cC: 6,7-dimethyl-8-ribityllumazine synthase
cD: 6,7-dimethyl-8-ribityllumazine synthase
cE: 6,7-dimethyl-8-ribityllumazine synthase
cX: 6,7-dimethyl-8-ribityllumazine synthase
dA: 6,7-dimethyl-8-ribityllumazine synthase
dB: 6,7-dimethyl-8-ribityllumazine synthase
dC: 6,7-dimethyl-8-ribityllumazine synthase
dD: 6,7-dimethyl-8-ribityllumazine synthase
dE: 6,7-dimethyl-8-ribityllumazine synthase
dX: 6,7-dimethyl-8-ribityllumazine synthase
eA: 6,7-dimethyl-8-ribityllumazine synthase
eB: 6,7-dimethyl-8-ribityllumazine synthase
eC: 6,7-dimethyl-8-ribityllumazine synthase
eD: 6,7-dimethyl-8-ribityllumazine synthase
eE: 6,7-dimethyl-8-ribityllumazine synthase
eX: 6,7-dimethyl-8-ribityllumazine synthase
fA: 6,7-dimethyl-8-ribityllumazine synthase
fB: 6,7-dimethyl-8-ribityllumazine synthase
fC: 6,7-dimethyl-8-ribityllumazine synthase
fD: 6,7-dimethyl-8-ribityllumazine synthase
fE: 6,7-dimethyl-8-ribityllumazine synthase
fX: 6,7-dimethyl-8-ribityllumazine synthase
gA: 6,7-dimethyl-8-ribityllumazine synthase
gB: 6,7-dimethyl-8-ribityllumazine synthase
gC: 6,7-dimethyl-8-ribityllumazine synthase
gD: 6,7-dimethyl-8-ribityllumazine synthase
gE: 6,7-dimethyl-8-ribityllumazine synthase
gX: 6,7-dimethyl-8-ribityllumazine synthase
hA: 6,7-dimethyl-8-ribityllumazine synthase
hB: 6,7-dimethyl-8-ribityllumazine synthase
hC: 6,7-dimethyl-8-ribityllumazine synthase
hD: 6,7-dimethyl-8-ribityllumazine synthase
hE: 6,7-dimethyl-8-ribityllumazine synthase
hX: 6,7-dimethyl-8-ribityllumazine synthase
iA: 6,7-dimethyl-8-ribityllumazine synthase
iB: 6,7-dimethyl-8-ribityllumazine synthase
iC: 6,7-dimethyl-8-ribityllumazine synthase
iD: 6,7-dimethyl-8-ribityllumazine synthase
iE: 6,7-dimethyl-8-ribityllumazine synthase
iX: 6,7-dimethyl-8-ribityllumazine synthase
jA: 6,7-dimethyl-8-ribityllumazine synthase
jB: 6,7-dimethyl-8-ribityllumazine synthase
jC: 6,7-dimethyl-8-ribityllumazine synthase
jD: 6,7-dimethyl-8-ribityllumazine synthase
jE: 6,7-dimethyl-8-ribityllumazine synthase
jX: 6,7-dimethyl-8-ribityllumazine synthase
kA: 6,7-dimethyl-8-ribityllumazine synthase
kB: 6,7-dimethyl-8-ribityllumazine synthase
kC: 6,7-dimethyl-8-ribityllumazine synthase
kD: 6,7-dimethyl-8-ribityllumazine synthase
kE: 6,7-dimethyl-8-ribityllumazine synthase
kX: 6,7-dimethyl-8-ribityllumazine synthase
lA: 6,7-dimethyl-8-ribityllumazine synthase
lB: 6,7-dimethyl-8-ribityllumazine synthase
lC: 6,7-dimethyl-8-ribityllumazine synthase
lD: 6,7-dimethyl-8-ribityllumazine synthase
lE: 6,7-dimethyl-8-ribityllumazine synthase
lX: 6,7-dimethyl-8-ribityllumazine synthase
mA: 6,7-dimethyl-8-ribityllumazine synthase
mB: 6,7-dimethyl-8-ribityllumazine synthase
mC: 6,7-dimethyl-8-ribityllumazine synthase
mD: 6,7-dimethyl-8-ribityllumazine synthase
mE: 6,7-dimethyl-8-ribityllumazine synthase
mX: 6,7-dimethyl-8-ribityllumazine synthase
nA: 6,7-dimethyl-8-ribityllumazine synthase
nB: 6,7-dimethyl-8-ribityllumazine synthase
nC: 6,7-dimethyl-8-ribityllumazine synthase
nD: 6,7-dimethyl-8-ribityllumazine synthase
nE: 6,7-dimethyl-8-ribityllumazine synthase
nX: 6,7-dimethyl-8-ribityllumazine synthase
oA: 6,7-dimethyl-8-ribityllumazine synthase
oB: 6,7-dimethyl-8-ribityllumazine synthase
oC: 6,7-dimethyl-8-ribityllumazine synthase
oD: 6,7-dimethyl-8-ribityllumazine synthase
oE: 6,7-dimethyl-8-ribityllumazine synthase
oX: 6,7-dimethyl-8-ribityllumazine synthase
pA: 6,7-dimethyl-8-ribityllumazine synthase
pB: 6,7-dimethyl-8-ribityllumazine synthase
pC: 6,7-dimethyl-8-ribityllumazine synthase
pD: 6,7-dimethyl-8-ribityllumazine synthase
pE: 6,7-dimethyl-8-ribityllumazine synthase
pX: 6,7-dimethyl-8-ribityllumazine synthase
qA: 6,7-dimethyl-8-ribityllumazine synthase
qB: 6,7-dimethyl-8-ribityllumazine synthase
qC: 6,7-dimethyl-8-ribityllumazine synthase
qD: 6,7-dimethyl-8-ribityllumazine synthase
qE: 6,7-dimethyl-8-ribityllumazine synthase
qX: 6,7-dimethyl-8-ribityllumazine synthase
rA: 6,7-dimethyl-8-ribityllumazine synthase
rB: 6,7-dimethyl-8-ribityllumazine synthase
rC: 6,7-dimethyl-8-ribityllumazine synthase
rD: 6,7-dimethyl-8-ribityllumazine synthase
rE: 6,7-dimethyl-8-ribityllumazine synthase
rX: 6,7-dimethyl-8-ribityllumazine synthase
sA: 6,7-dimethyl-8-ribityllumazine synthase
sB: 6,7-dimethyl-8-ribityllumazine synthase
sC: 6,7-dimethyl-8-ribityllumazine synthase
sD: 6,7-dimethyl-8-ribityllumazine synthase
sE: 6,7-dimethyl-8-ribityllumazine synthase
sX: 6,7-dimethyl-8-ribityllumazine synthase
tA: 6,7-dimethyl-8-ribityllumazine synthase
tB: 6,7-dimethyl-8-ribityllumazine synthase
tC: 6,7-dimethyl-8-ribityllumazine synthase
tD: 6,7-dimethyl-8-ribityllumazine synthase
tE: 6,7-dimethyl-8-ribityllumazine synthase
tX: 6,7-dimethyl-8-ribityllumazine synthase
uA: 6,7-dimethyl-8-ribityllumazine synthase
uB: 6,7-dimethyl-8-ribityllumazine synthase
uC: 6,7-dimethyl-8-ribityllumazine synthase
uD: 6,7-dimethyl-8-ribityllumazine synthase
uE: 6,7-dimethyl-8-ribityllumazine synthase
uX: 6,7-dimethyl-8-ribityllumazine synthase
vA: 6,7-dimethyl-8-ribityllumazine synthase
vB: 6,7-dimethyl-8-ribityllumazine synthase
vC: 6,7-dimethyl-8-ribityllumazine synthase
vD: 6,7-dimethyl-8-ribityllumazine synthase
vE: 6,7-dimethyl-8-ribityllumazine synthase
vX: 6,7-dimethyl-8-ribityllumazine synthase
wA: 6,7-dimethyl-8-ribityllumazine synthase
wB: 6,7-dimethyl-8-ribityllumazine synthase
wC: 6,7-dimethyl-8-ribityllumazine synthase
wD: 6,7-dimethyl-8-ribityllumazine synthase
wE: 6,7-dimethyl-8-ribityllumazine synthase
wX: 6,7-dimethyl-8-ribityllumazine synthase
xA: 6,7-dimethyl-8-ribityllumazine synthase
xB: 6,7-dimethyl-8-ribityllumazine synthase
xC: 6,7-dimethyl-8-ribityllumazine synthase
xD: 6,7-dimethyl-8-ribityllumazine synthase
xE: 6,7-dimethyl-8-ribityllumazine synthase
xX: 6,7-dimethyl-8-ribityllumazine synthase


Theoretical massNumber of molelcules
Total (without water)6,325,148360
Polymers6,325,148360
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA

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Components

#1: Protein ...
6,7-dimethyl-8-ribityllumazine synthase / Lumazine synthase / Lumazine synthase


Mass: 17569.855 Da / Num. of mol.: 360
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Aquifex aeolicus (bacteria) / Strain: VF5 / Gene: ribH, aq_132 / Production host: Escherichia coli (E. coli)
References: UniProt: O66529, 6,7-dimethyl-8-ribityllumazine synthase

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: AaLS-13 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Aquifex aeolicus (bacteria)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7.8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 4 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

EM software
IDNameVersionCategory
4CTFFIND3CTF correction
5RELION1.4CTF correction
11RELION1.4initial Euler assignment
12RELION1.4final Euler assignment
13RELION1.4classification
14RELION1.43D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: I (icosahedral)
3D reconstructionResolution: 5.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 9900 / Symmetry type: POINT

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