[English] 日本語
Yorodumi- PDB-5m1q: Crystal structure of the large terminase nuclease from thermophil... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5m1q | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Zinc | |||||||||
Components | Phage terminase large subunit | |||||||||
Keywords | VIRAL PROTEIN / large terminase / nuclease domain | |||||||||
Function / homology | Function and homology information viral terminase, large subunit / viral DNA genome packaging / Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / chromosome organization / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / endonuclease activity / ATP hydrolysis activity / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Thermus phage G20c (virus) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.45 Å | |||||||||
Authors | Xu, R.G. / Jenkins, H.T. / Chechik, M. / Blagova, E.V. / Greive, S.J. / Antson, A.A. | |||||||||
Funding support | United Kingdom, 2items
| |||||||||
Citation | Journal: Nucleic Acids Res. / Year: 2017 Title: Viral genome packaging terminase cleaves DNA using the canonical RuvC-like two-metal catalysis mechanism. Authors: Xu, R.G. / Jenkins, H.T. / Chechik, M. / Blagova, E.V. / Lopatina, A. / Klimuk, E. / Minakhin, L. / Severinov, K. / Greive, S.J. / Antson, A.A. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 5m1q.cif.gz | 48.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb5m1q.ent.gz | 35.9 KB | Display | PDB format |
PDBx/mmJSON format | 5m1q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m1/5m1q ftp://data.pdbj.org/pub/pdb/validation_reports/m1/5m1q | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20818.363 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: scientific_name: 'Thermus phage G20c' ncbi_taxonomy_id: 1406341 The same as entry.id 4XVN Source: (gene. exp.) Thermus phage G20c (virus) / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1L4BKS3*PLUS | ||
---|---|---|---|
#2: Chemical | ChemComp-ZN / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.37 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.01 M Zinc chloride, 0.1 M MES pH 6.0, 20% PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å | ||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 7, 2012 | ||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 1.45→45.6 Å / Num. obs: 33635 / % possible obs: 99.7 % / Redundancy: 4.5 % / CC1/2: 0.997 / Rmerge(I) obs: 0.086 / Rpim(I) all: 0.045 / Rrim(I) all: 0.097 / Net I/σ(I): 8.2 / Num. measured all: 151672 | ||||||||||||||||||
Reflection shell |
|
-Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: in house model Resolution: 1.45→45.6 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.961 / SU B: 1.601 / SU ML: 0.058 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.07 / ESU R Free: 0.066 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 57.46 Å2 / Biso mean: 23.601 Å2 / Biso min: 10.28 Å2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.45→45.6 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.45→1.488 Å / Total num. of bins used: 20
|