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Yorodumi- PDB-5ja5: Crystal structure of the rice Topless related protein 2 (TPR2) N-... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5ja5 | ||||||||||||
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Title | Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A and L130A mutant in complex with rice D53 repressor EAR peptide motif | ||||||||||||
Components |
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Keywords | TRANSCRIPTION / TRANSCRIPTION REPRESSION / TRANSCRIPTIONAL COREPRESSOR TOPLESS / ALPHA-HELICAL STRUCTURE / TETRAMER / TRANSCRIPTIONAL REPRESSOR D53 | ||||||||||||
Function / homology | Function and homology information positive regulation of pattern recognition receptor signaling pathway / response to strigolactone / protein sequestering activity / regulation of DNA-templated transcription / nucleus / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Oryza sativa (Asian cultivated rice) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||||||||
Authors | Ke, J. / Ma, H. / Gu, X. / Brunzelle, J.S. / Xu, H.E. / Melcher, K. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: Sci Adv / Year: 2017 Title: A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex. Authors: Ma, H. / Duan, J. / Ke, J. / He, Y. / Gu, X. / Xu, T.H. / Yu, H. / Wang, Y. / Brunzelle, J.S. / Jiang, Y. / Rothbart, S.B. / Xu, H.E. / Li, J. / Melcher, K. #1: Journal: Sci Adv / Year: 2015 Title: Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Authors: Ke, J. / Ma, H. / Gu, X. / Thelen, A. / Brunzelle, J.S. / Li, J. / Xu, H.E. / Melcher, K. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ja5.cif.gz | 62.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ja5.ent.gz | 44 KB | Display | PDB format |
PDBx/mmJSON format | 5ja5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ja/5ja5 ftp://data.pdbj.org/pub/pdb/validation_reports/ja/5ja5 | HTTPS FTP |
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-Related structure data
Related structure data | 5j9kC 5jgcC 5jhpC 4zheS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 24819.451 Da / Num. of mol.: 1 / Fragment: N-terminal topless domain (UNP residues 1-209) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Oryza sativa (Asian cultivated rice) Gene: TPR1, ASPR2, TPR2, Os01g0254100, LOC_Os01g15020, OsJ_01134, OSNPB_010254100, P0705D01.10-1 Production host: Escherichia coli (E. coli) / References: UniProt: Q5NBT9 |
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#2: Protein/peptide | Mass: 1864.961 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Oryza sativa (Asian cultivated rice) / References: UniProt: Q2RBP2*PLUS |
#3: Chemical | ChemComp-ZN / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.9 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.8 Details: 20% w/v Polyethylene glycol 3350, 0.2 M Potassium phosphate monobasic, pH 4.8 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: liquid nitrogen stream |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1.078 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 7, 2015 |
Radiation | Monochromator: Ni FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.078 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 20502 / % possible obs: 100 % / Redundancy: 15.6 % / CC1/2: 1 / Rmerge(I) obs: 0.07 / Net I/σ(I): 25.9 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 16 % / Rmerge(I) obs: 0.893 / Mean I/σ(I) obs: 4 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4ZHE Resolution: 2→29.612 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.48 / Phase error: 23.79
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→29.612 Å
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Refine LS restraints |
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LS refinement shell |
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