PDB-5dqt: Crystal Structure of Cas-DNA-22 complex

ID or keywords:

Entry

Database: PDB / ID: 5dqt

Title

Crystal Structure of Cas-DNA-22 complex

Components

CRISPR-associated endonuclease Cas1
CRISPR-associated endoribonuclease Cas2
DNA (33-MER)
DNA (34-MER)

Keywords

HYDROLASE/DNA /

protein-DNA complex / HYDROLASE-DNA complex

Function / homology

Function and homology information

CRISPR-cas system / crossover junction DNA endonuclease activity / 5'-flap endonuclease activity / maintenance of CRISPR repeat elements / defense response to virus /

endonuclease activity /

Hydrolases; Acting on ester bonds /

DNA repair / DNA damage response / protein homodimerization activity ...
Similarity search - Function

Biological species

Escherichia coli K12 (bacteria)

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.1 Å

Authors

Wang, J. / Li, J. / Zhao, H. / Sheng, G. / Wang, M. / Yin, M. / Wang, Y.

Funding support

China, 1items

Citation

Journal: Cell / Year: 2015
Title: Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
Authors: Wang, J. / Li, J. / Zhao, H. / Sheng, G. / Wang, M. / Yin, M. / Wang, Y.

History

Deposition	Sep 15, 2015	Deposition site: RCSB / Processing site: PDBJ
Revision 1.0	Nov 11, 2015	Provider: repository / Type: Initial release
Revision 1.1	Nov 18, 2015	Group: Database references
Revision 1.2	Mar 20, 2024	Group: Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_oper_list Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5dqt.cif.gz	1.1 MB	Display	PDBx/mmCIF format
PDB format	pdb5dqt.ent.gz	944.2 KB	Display	PDB format
PDBx/mmJSON format	5dqt.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/dq/5dqt ftp://data.pdbj.org/pub/pdb/validation_reports/dq/5dqt	HTTPS FTP

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Related structure data

Related structure data	5dljC 5dquC 5dqzC C: citing same article (ref.)
Similar structure data	5ds4-d 5dqz-d 5dlj-d 4o5p-d 21103 3gwc-d 5ds6-d 4fyr-1 1ibr-d 4z7i-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#181 - Jan 2015 Cascade and CRISPR similarity (11) #67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #253 - Jan 2021 Expressome similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #84 - Dec 2006 Transposase similarity (1) #135 - Mar 2011 Integrase similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #125 - May 2010 Parvoviruses similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #131 - Nov 2010 Inteins similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

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Deposited unit

D: CRISPR-associated endonuclease Cas1

C: CRISPR-associated endonuclease Cas1

A: CRISPR-associated endonuclease Cas1

B: CRISPR-associated endonuclease Cas1

F: CRISPR-associated endoribonuclease Cas2

E: CRISPR-associated endoribonuclease Cas2

L: CRISPR-associated endonuclease Cas1

K: CRISPR-associated endonuclease Cas1

I: CRISPR-associated endonuclease Cas1

J: CRISPR-associated endonuclease Cas1

N: CRISPR-associated endoribonuclease Cas2

M: CRISPR-associated endoribonuclease Cas2

G: DNA (34-MER)

H: DNA (33-MER)

O: DNA (34-MER)

P: DNA (33-MER)

349 kDa, 16 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

D: CRISPR-associated endonuclease Cas1

C: CRISPR-associated endonuclease Cas1

A: CRISPR-associated endonuclease Cas1

B: CRISPR-associated endonuclease Cas1

F: CRISPR-associated endoribonuclease Cas2

E: CRISPR-associated endoribonuclease Cas2

G: DNA (34-MER)

H: DNA (33-MER)

defined by author&software
174 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

L: CRISPR-associated endonuclease Cas1

K: CRISPR-associated endonuclease Cas1

I: CRISPR-associated endonuclease Cas1

J: CRISPR-associated endonuclease Cas1

N: CRISPR-associated endoribonuclease Cas2

M: CRISPR-associated endoribonuclease Cas2

O: DNA (34-MER)

P: DNA (33-MER)

defined by author&software
174 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	71.434, 194.955, 195.202
Angle α, β, γ (deg.)	90.000, 90.220, 90.000
Int Tables number	4
Space group name H-M	P12₁1

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:
NCS ensembles :

ID
1
2
3
4

#1: Protein	CRISPR-associated endonuclease Cas1 Mass: 33235.418 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K12 (bacteria) / Strain: K12 / Gene: ygbT, cas1, b2755, JW2725 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: Q46896, Hydrolases; Acting on ester bonds
#2: Protein	CRISPR-associated endoribonuclease Cas2 Mass: 10527.212 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K12 (bacteria) / Strain: K12 / Gene: ygbF, cas2, b2754, JW5438 / Production host: Escherichia coli (E. coli) References: UniProt: P45956, Hydrolases; Acting on ester bonds
#3: DNA chain	DNA (34-MER) Mass: 10473.714 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA-22-F / Source: (synth.) Escherichia coli (E. coli)
#4: DNA chain	DNA (33-MER) Mass: 10027.452 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA-22-R / Source: (synth.) Escherichia coli (E. coli)

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 4.14 Å³/Da / Density % sol: 70.28 %
Crystal grow	Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 5% PEG8000,50mM Mes,200mM Potassium chloride,10mM Magnesium chloride

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

SSRF

/ Beamline: BL17U / Wavelength: 0.979 Å

Detector

Type: ADSC QUANTUM 315 / Detector: CCD / Date: May 30, 2015 / Details: Mono-chromator and mirrors

Radiation

Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.979 Å / Relative weight: 1

Reflection

Resolution: 3.1→50 Å / Num. obs: 91533 / % possible obs: 93.3 % / Redundancy: 3 % / B_iso Wilson estimate: 98.11 Å² / R_merge(I) obs: 0.082 / R_pim(I) all: 0.051 / R_rim(I) all: 0.097 / Χ²: 1.34 / Net I/av σ(I): 14.088 / Net I/σ(I): 11.2 / Num. measured all: 274007

Reflection shell

Diffraction-ID: 1 / Rejects: 0

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Num. unique all	CC_1/2	R_pim(I) all	R_rim(I) all	Χ²	% possible all
3.1-3.15	2.8	0.67	4756	0.355	0.451	0.813	0.659	96.7
3.15-3.21	2.8	0.61	4692	0.459	0.41	0.74	0.677	96.9
3.21-3.27	2.8	0.592	4753	0.522	0.394	0.715	0.776	96.2
3.27-3.34	2.8	0.5	4622	0.638	0.338	0.607	0.703	95.8
3.34-3.41	2.8	0.432	4745	0.725	0.29	0.523	0.734	95.7
3.41-3.49	2.7	0.376	4442	0.83	0.253	0.456	0.8	92.1
3.49-3.58	2.4	0.357	3963	0.847	0.25	0.439	0.807	80.7
3.58-3.68	2.7	0.248	3903	0.88	0.16	0.297	1.146	80.2
3.68-3.78	3.1	0.166	4199	0.923	0.102	0.196	0.975	84.9
3.78-3.91	2.8	0.218	4334	0.923	0.14	0.26	0.97	89.4
3.91-4.04	2.6	0.168	4227	0.958	0.111	0.202	1.85	85.8
4.04-4.21	3	0.131	4553	0.976	0.082	0.156	0.981	93
4.21-4.4	3.1	0.109	4671	0.984	0.068	0.129	1.043	95.6
4.4-4.63	3.2	0.088	4686	0.99	0.054	0.104	1.065	95.8
4.63-4.92	3.2	0.081	4731	0.991	0.05	0.096	1.303	96.7
4.92-5.3	3.3	0.083	4816	0.989	0.05	0.097	1.378	97.3
5.3-5.83	3.4	0.076	4821	0.992	0.046	0.089	1.652	98.2
5.83-6.67	3.4	0.071	4857	0.992	0.043	0.083	2.037	98.7
6.67-8.4	3.4	0.053	4904	0.995	0.032	0.062	2.426	99.2
8.4-50	3.4	0.044	4858	0.995	0.026	0.051	3.228	97

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Processing

Software

Name	Version	Classification
PHENIX	1.9_1692	refinement
HKL-2000		data scaling
PDB_EXTRACT	3.15	data extraction
PHASER		phasing
HKL-2000		data processing
HKL-2000		data reduction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 3.1→48.8 Å / SU ML: 0.45 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 27.98 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2583	4510	5.01 %
R_work	0.2106	85475	-
obs	0.213	89985	93.16 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 227.52 Å² / B_iso mean: 110.3012 Å² / B_iso min: 42.75 Å²

Refinement step

Cycle: final / Resolution: 3.1→48.8 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	19288	2668	0	0	21956
Num. residues	-	-	-	-	2673

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	22632
X-RAY DIFFRACTION	f_angle_d	1.291	31292
X-RAY DIFFRACTION	f_chiral_restr	0.051	3646
X-RAY DIFFRACTION	f_plane_restr	0.007	3561
X-RAY DIFFRACTION	f_dihedral_angle_d	18.358	8469

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Rms	Type
1	1	A	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	2	B	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	3	C	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	4	D	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	5	I	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	6	J	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	7	K	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
1	8	L	9852	X-RAY DIFFRACTION	11.347	TORSIONAL
2	1	E	1693	X-RAY DIFFRACTION	11.347	TORSIONAL
2	2	F	1693	X-RAY DIFFRACTION	11.347	TORSIONAL
2	3	M	1693	X-RAY DIFFRACTION	11.347	TORSIONAL
2	4	N	1693	X-RAY DIFFRACTION	11.347	TORSIONAL
3	1	G	654	X-RAY DIFFRACTION	11.347	TORSIONAL
3	2	O	654	X-RAY DIFFRACTION	11.347	TORSIONAL
4	1	H	630	X-RAY DIFFRACTION	11.347	TORSIONAL
4	2	P	630	X-RAY DIFFRACTION	11.347	TORSIONAL

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
3.1-3.1352	0.3852	142	0.3333	2908	3050	97
3.1352-3.1721	0.3565	149	0.3326	2896	3045	97
3.1721-3.2108	0.3488	157	0.3084	2975	3132	97
3.2108-3.2514	0.366	156	0.3053	3019	3175	96
3.2514-3.2942	0.365	134	0.2979	2872	3006	96
3.2942-3.3393	0.3393	147	0.2854	2894	3041	96
3.3393-3.387	0.3469	152	0.2913	2940	3092	95
3.387-3.4375	0.3633	176	0.3093	2830	3006	94
3.4375-3.4912	0.4019	174	0.3748	2732	2906	90
3.4912-3.5485	0.4834	137	0.4221	2495	2632	81
3.5485-3.6096	0.4937	102	0.4672	2032	2134	66
3.6096-3.6752	0.4847	150	0.435	2919	3069	96
3.6752-3.7459	0.4821	145	0.4028	2689	2834	88
3.7459-3.8223	0.3207	129	0.3361	2303	2432	76
3.8223-3.9054	0.2919	157	0.2346	2944	3101	96
3.9054-3.9962	0.2766	149	0.244	2544	2693	83
3.9962-4.0961	0.2644	130	0.212	2730	2860	90
4.0961-4.2068	0.2321	153	0.1863	2934	3087	95
4.2068-4.3305	0.2362	152	0.1869	2897	3049	95
4.3305-4.4702	0.2187	140	0.1713	2984	3124	96
4.4702-4.6299	0.2025	167	0.1579	2892	3059	96
4.6299-4.8151	0.1991	158	0.1502	2958	3116	97
4.8151-5.034	0.2174	170	0.1658	2940	3110	97
5.034-5.2991	0.2364	157	0.1671	2973	3130	98
5.2991-5.6307	0.2317	152	0.1639	3034	3186	98
5.6307-6.0647	0.265	152	0.1819	3047	3199	99
6.0647-6.6736	0.2549	149	0.183	3024	3173	99
6.6736-7.6361	0.2226	175	0.1614	3064	3239	99
7.6361-9.6087	0.169	145	0.1292	3062	3207	99
9.6087-48.8062	0.1657	154	0.1504	2944	3098	94

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.6732	-0.8287	3.1737	5.5053	3.3359	3.9165	-0.3219	0.1328	0.5333	-0.3371	0.0312	0.3464	-0.8969	-0.1707	0.2169	0.8632	0.0448	0.0555	0.4499	0.1978	0.6935	104.5172	85.4237	71.4799
2	2.8285	-0.9877	1.9411	0.2369	-1.4507	1.427	0.046	0.1534	0.2015	0.1415	-0.1583	-0.3694	-0.0169	0.4165	-0.0185	0.8	0.0647	0.0381	1.0251	-0.0025	1.0201	132.1271	73.3674	67.1869
3	0.8726	1.3892	1.8737	4.5831	-0.8188	6.6005	-0.0698	0.0495	0.1026	-0.2132	0.2782	-0.6599	0.176	0.9584	-0.0083	0.4664	0.0826	0.0491	0.8218	0.1054	0.8761	128.5835	81.3052	70.6873
4	3.1552	0.5269	-0.7131	3.8748	0.2628	4.9001	0.1101	0.2058	0.1217	-0.0775	-0.1573	-0.2639	0.4022	-0.0251	0.0778	0.7482	0.0816	0.005	0.5545	0.0866	0.5837	101.6401	67.5096	81.0488
5	8.5236	-5.1922	-3.9029	6.2417	4.2375	6.7288	0.171	0.308	-0.2786	-0.2621	-0.4201	-0.3069	-0.1216	-0.2634	0.2395	0.8352	0.0672	-0.1099	0.4735	0.0095	0.6256	111.3589	63.6736	62.241
6	3.2559	-1.547	-1.4124	6.2642	1.8429	3.703	0.933	0.6015	-0.7462	-0.8756	-0.5173	0.4015	0.0377	0.1972	-0.4918	1.1904	0.0459	-0.0797	0.9532	-0.0279	0.7609	100.6478	50.9978	47.568
7	4.7938	2.1567	3.5946	5.4219	-1.3058	4.4345	0.02	-0.108	0.7235	0.0693	-0.0518	0.4639	0.32	-0.2124	0.0302	0.7268	0.084	-0.0199	0.6592	-0.0062	0.6119	96.427	61.2459	58.5659
8	8.275	0.1675	-1.0914	2.0469	-2.5046	3.5971	-0.4493	-0.6938	-0.2926	0.2315	0.3142	0.159	0.487	-0.2739	-0.0529	0.8855	-0.0513	-0.1375	0.7532	-0.1384	0.6614	95.5552	53.3708	65.4132
9	4.3251	-0.3052	-0.8043	3.5775	2.0944	2.6158	0.4644	0.6028	0.1385	-0.0193	0.0275	0.2179	0.3726	0.1803	-0.3325	0.931	0.0197	-0.1649	0.8356	0.0721	0.6192	91.1214	25.7252	134.971
10	5.1932	0.0121	1.8246	4.5654	3.211	4.2906	0.5217	-1.291	-0.1915	1.5673	-0.7097	-0.6489	1.818	-0.894	-0.4057	1.0285	-0.0659	-0.0953	0.7754	0.1706	0.7501	90.2741	24.194	136.7556
11	4.6248	-1.0198	-0.4014	3.2355	2.6368	2.773	0.1201	-0.8887	-0.8588	0.5608	0.2248	0.3241	0.3283	0.4579	-0.3152	0.8001	-0.0575	-0.1065	0.63	0.1815	0.5113	91.909	23.9055	126.9241
12	4.4932	1.655	-0.7302	4.4254	2.1432	2.3172	0.2779	-0.3121	-0.2738	0.5291	-0.2205	0.6078	0.2796	-0.4639	0.0049	0.704	-0.0319	-0.1859	0.8461	0.2558	1.0088	77.4954	15.7478	118.6506
13	6.8649	0.7108	-0.4783	3.7834	0.1967	3.0379	-0.1808	-0.7211	-0.9626	0.7042	0.4268	0.149	1.1238	-0.8229	0.2972	0.7674	-0.1374	-0.0129	1.2647	0.0078	1.2809	57.0028	9.7262	120.2192
14	3.596	1.8442	1.6101	5.9074	0.2361	0.4581	0.362	-0.5485	0.7236	0.2254	-0.3157	0.1375	-0.2545	-0.1452	-0.6828	1.0221	0.0594	0.2272	1.4417	0.2533	1.485	54.2118	31.3967	120.2932
15	1.3591	-2.0466	0.1279	7.351	1.7209	3.1308	0.3604	-0.4056	-0.1655	-0.6246	-0.0901	0.4335	-0.3406	-0.5817	-0.1403	0.5925	0.0105	-0.0406	0.9268	0.0273	1.0863	68.6968	24.4952	119.9746
16	2.0734	-2.3274	-0.2293	6.6226	2.0192	2.751	-0.6703	-1.1435	0.2701	-1.773	1.3666	1.0796	0.1074	-0.2209	0.0673	0.0247	0.2414	-0.0084	1.4091	0.1613	1.0875	61.8718	30.3103	127.4144
17	3.3233	-2.0799	-0.0573	6.1132	0.7686	4.696	-0.0881	-0.5299	0.2533	-0.2857	0.3163	-0.622	-0.7805	-0.1574	-0.2409	0.9316	-0.0437	0.0615	1.0007	0.0304	0.7061	70.379	33.8114	132.9305
18	0.8988	-1.7867	-0.0275	4.3065	1.1287	2.7951	-0.0132	-0.277	0.0012	-0.2616	0.0363	0.623	1.105	-1.1114	-0.028	0.809	-0.0715	0.0674	0.982	0.23	0.787	70.5745	9.3403	126.2133
19	2.0785	0.4256	-1.3884	5.6877	-1.6811	5.3711	0.3305	-0.0851	-0.1897	-0.3159	-0.0644	-0.2772	-0.1674	-0.0059	-0.2066	0.5314	0.0246	-0.1478	0.6039	0.0654	0.5764	93.7776	34.2241	114.3987
20	6.4115	2.6306	0.9467	4.556	2.7205	3.1201	0.0825	0.268	-0.3218	-0.0949	0.2295	0.0428	0.2884	-0.2627	0.1463	0.7158	0.0636	-0.0074	0.5359	-0.008	0.6031	84.1837	15.4569	110.4191
21	7.2566	0.1724	-1.6139	2.8608	0.5037	4.966	0.2974	0.5592	-0.3892	-0.4381	-0.285	0.3454	0.401	0.2491	0.187	1.1349	0.0121	-0.0611	0.8226	0.0299	1.0399	94.8727	0.337	97.7373
22	7.5469	0.001	-0.7321	3.8321	-1.447	3.7486	-0.2053	0.0897	-0.5786	0.8672	-0.4988	-1.003	-1.3974	0.0257	0.1307	1.2541	0.1549	-0.0994	0.9961	0.2792	1.0916	112.2178	8.4417	113.2527
23	9.238	-0.4283	-0.0788	2.4215	-0.199	2.1313	0.4852	-0.4716	-0.5642	-0.2221	-0.2006	0.0578	-0.2266	-0.1016	-0.1634	0.8339	0.0333	-0.0859	0.5968	-0.0085	0.749	94.8193	12.9221	106.8864
24	3.2725	-2.0201	0.7	4.594	-0.3751	3.549	-0.0862	0.3792	1.131	-0.2615	0.0453	-0.2383	0.1026	0.1394	0.0276	0.883	0.041	-0.0525	0.5825	-0.0413	0.8261	99.2081	18.3565	100.4408
25	8.4732	-2.7235	-0.9689	2.3786	-1.0763	2.5723	-0.1072	-0.0527	0.3959	0.7883	0.0639	0.5482	0.5283	0.1976	0.0735	0.8186	0.1121	-0.0589	0.5162	0.196	0.8203	91.5964	49.9574	101.6378
26	3.1165	-0.6453	2.8683	2.0137	-2.7848	5.2721	-0.2343	0.504	-0.2175	-0.0805	0.9828	1.2514	-0.344	0.3949	0.025	0.8466	0.1769	0.1055	0.6253	0.0083	0.7707	88.2796	45.6826	110.1353
27	2.8115	4.0384	0.1453	8.354	-2.0703	3.1982	-0.4632	0.9037	-0.6566	0.7694	0.9253	0.3485	-1.2692	0.157	-0.6155	0.8582	-0.0641	0.19	0.6943	-0.0825	0.5699	96.1944	46.1406	103.3469
28	6.5381	-3.8027	-4.8699	9.5536	1.8746	5.9898	0.2927	0.1192	-0.4617	-1.2032	0.242	0.9367	0.1794	-0.797	-0.2222	0.4861	-0.1084	-0.1487	0.8274	0.0968	0.6553	82.8828	44.6935	100.3932
29	5.3044	-0.1009	0.9274	3.1312	2.7818	2.9197	-0.1358	-1.0281	0.2521	-0.5846	0.6118	1.6085	-0.6946	0.4497	0.27	0.9146	0.0921	-0.0922	0.7192	0.2307	0.9903	86.7535	53.3465	100.765
30	3.2275	0.3835	0.5964	5.3904	2.7137	2.3012	0.0169	1.6002	0.4835	-1.5829	0.0545	0.3787	-1.0488	-1.132	0.0291	0.9934	0.065	-0.1539	0.961	0.1433	0.944	90.0955	51.2449	88.7059
31	4.3781	-4.0029	-5.0355	8.3914	0.8677	8.3058	-0.3715	-0.5452	-0.9264	0.1749	0.4499	-0.0299	1.0328	-0.2929	-0.8341	1.0686	0.1355	-0.1864	0.585	0.1252	0.9049	88.1936	63.6202	93.7318
32	2.5057	-1.4461	-1.8904	2.1428	1.9471	1.7115	0.8213	0.7883	-0.8745	-0.4682	-0.7963	-2.324	0.6033	0.6008	0.1119	1.2595	-0.0048	-0.2418	0.6709	0.1705	1.2153	90.5227	63.8463	88.8149
33	9.0397	2.9501	3.2263	2.6927	-0.5198	8.1084	-0.1242	-1.2875	0.4164	-0.6493	-0.573	0.3169	-1.6152	-0.5128	0.3204	0.8441	0.0847	-0.0082	0.707	0.1483	0.5887	103.999	54.737	96.9176
34	6.6721	0.2722	-0.0569	3.5612	-2.5755	3.6987	0.1756	0.0167	-0.2171	-0.1869	-0.526	0.312	-0.3901	0.4881	0.3018	0.9445	-0.1418	0.0265	0.6722	-0.0846	0.6489	103.0734	59.4745	92.8591
35	4.1482	1.8142	0.6726	2.5414	1.5407	7.0257	-0.302	-0.2788	-0.7854	-0.5905	-0.1848	-0.2954	1.6356	0.9227	0.324	0.8533	0.2794	0.0378	0.6504	0.1665	0.6858	112.7473	53.4095	91.6589
36	7.8513	3.7353	0.4877	2.6536	1.9345	5.8364	-0.0234	-0.1291	-0.5692	0.2387	-0.2557	-0.9917	-0.0919	0.2741	-0.094	0.7	0.0957	0.0681	0.8777	0.1839	0.8474	106.7067	47.5394	98.7956
37	8.4345	3.0099	4.5433	4.5947	5.6458	7.1013	-0.5493	-1.7731	-2.3289	1.1388	-0.02	-2.6687	0.4963	-0.0666	-0.0797	1.0234	0.0811	0.0343	0.7483	-0.0298	1.1564	108.0606	46.3618	100.3919
38	4.6645	2.5773	2.5556	4.6828	-2.2371	5.3239	-0.5236	0.2238	0.6048	0.097	-0.6885	0.1057	-1.7811	0.308	1.1695	0.9481	-0.0449	-0.2199	0.867	0.144	0.7896	107.4435	46.7785	110.6524
39	4.2293	3.3593	0.9358	6.5269	5.3486	5.6508	0.732	0.5016	0.0911	-1.0401	-1.0249	-1.2911	-0.4365	-2.632	0.0111	0.9225	0.2187	-0.1215	1.1684	0.1561	0.6439	105.1835	41.7616	110.8326
40	5.3235	0.386	0.5776	4.3889	-1.4989	5.328	-0.0174	0.2516	-0.575	-0.0729	0.1608	-0.0048	1.2051	-0.4204	-0.1955	0.6734	0.0073	-0.0297	0.531	-0.0993	0.7431	71.5243	16.8088	169.7044
41	6.4002	2.8135	2.6807	3.942	0.6368	5.8319	-0.8391	0.335	-0.7086	-0.5598	0.7057	1.0182	0.0642	-1.3477	-0.1685	0.5997	0.1162	-0.1312	1.0968	-0.1808	0.805	59.0335	32.4453	153.5106
42	2.366	0.41	-0.2678	1.2663	0.959	0.0806	-0.0832	0.1124	0.3795	0.1535	-0.2041	0.7079	0.1369	-0.3879	0.2323	0.7665	0.0137	-0.0412	0.9397	-0.0505	1.1235	42.2487	27.4473	166.952
43	3.1036	0.4878	-1.1202	3.8744	0.97	3.4008	-0.3562	-0.1302	-0.1416	0.3293	-0.0834	0.5446	0.1978	-0.6174	0.2807	0.7086	0.0137	0.0081	0.699	-0.1769	1.0432	49.6602	18.2581	170.1859
44	3.8657	-0.4226	-1.567	3.0838	1.4645	7.1875	0.0372	0.1498	0.0198	-0.1984	-0.1882	-0.0392	0.1775	0.2285	0.1504	0.8313	0.0257	-0.0402	0.5055	-0.0331	0.6101	75.9095	33.7786	178.6722
45	5.906	-2.2971	3.4337	3.0667	-2.6481	4.4702	0.1278	0.1767	0.1852	-0.1888	-0.4469	0.5702	-0.6806	0.0148	-0.161	1.0043	0.0948	0.0096	0.8136	0.0256	0.8154	65.6328	38.0402	159.9033
46	4.5236	-0.3948	-0.1988	5.3654	-0.8356	6.0027	-0.1181	0.5712	1.4745	-0.7315	0.0167	0.6779	-0.6396	-0.8948	0.0493	1.2273	0.0547	-0.0041	1.0693	0.064	0.9575	70.543	52.2259	144.5691
47	8.7198	-0.4963	4.6195	2.9835	-0.5666	5.0349	1.2903	0.6374	0.5964	-0.3836	-0.9962	-0.0829	0.4969	0.3449	-0.0358	1.2247	-0.0181	0.1124	1.1035	-0.101	0.8744	84.8838	48.2032	144.9733
48	4.2921	0.4079	0.3582	5.4941	-0.0808	3.7865	0.2414	-0.341	0.622	1.5147	-0.0012	-0.2522	0.6405	0.5342	-0.0513	1.3674	0.0723	0.1457	1.1209	-0.1378	1.1294	94.4079	35.0691	152.8364
49	5.0884	1.7014	0.2264	2.7789	-0.4854	2.6306	0.7383	0.4271	-0.6904	0.37	-0.3731	0.0799	-0.2625	0.0703	-0.1195	0.5868	0.0802	-0.0738	0.7905	-0.0667	0.6626	76.5433	39.639	158.2607
50	7.4307	2.6565	1.0164	4.471	0.5016	2.9924	0.2557	0.8711	0.4708	-0.6373	-0.5332	-1.0034	-0.111	1.2261	-0.0378	0.8351	0.1486	0.0391	0.9828	0.1586	0.7266	86.5564	44.5746	158.6205
51	3.8659	0.0775	1.7404	5.8212	1.6748	3.9154	-0.8782	-0.8127	1.6323	0.4832	-0.0708	0.0623	-1.105	-0.0921	0.1156	1.0278	0.0217	0.2825	0.7653	-0.0234	0.7954	75.1597	49.9709	163.2987
52	6.4973	-0.9803	0.0221	3.0717	-3.6919	3.6813	0.2333	-0.7054	0.2861	0.7858	-0.1784	0.3366	-0.8842	-0.2642	0.0548	0.7662	-0.0389	0.071	0.6408	-0.164	0.5193	86.0697	76.6758	230.1397
53	4.1414	0.8953	0.7345	0.2045	0.1641	1.0711	0.1744	-0.0407	0.0525	-0.0183	-0.208	-0.3613	0.0392	0.6395	-0.0639	0.7156	0.0514	0.0679	1.1748	0.0387	1.1723	114.3055	85.9189	217.3007
54	2.3371	0.1303	-0.1159	3.5194	-2.9431	2.4942	0.0724	-0.2034	-0.248	-1.1608	-0.3008	0.1464	0.5987	-0.4694	-0.408	1.3806	0.2735	-0.0743	1.3799	-0.2447	1.2358	121.0362	63.1456	217.7568
55	2.3807	0.6359	0.1963	4.2143	0.9595	3.6964	0.0327	-0.514	0.0026	-0.3558	0.1591	-0.5809	0.388	0.3697	-0.182	0.5343	0.0544	0.0948	0.9669	-0.0398	0.7852	109.7034	76.9596	223.1625
56	3.3295	-0.6793	-0.7748	8.3763	0.0939	5.2627	0.0935	-0.2402	0.2208	-0.0161	-0.0433	0.273	0.597	0.0583	-0.0157	0.6644	-0.0263	0.0471	0.5215	-0.0452	0.483	83.1992	67.1155	212.1598
57	9.0772	4.3905	-3.0619	7.598	-3.586	5.9358	0.0843	-0.1532	0.1188	0.129	0.0862	-0.2269	0.0363	0.644	-0.3398	0.7909	0.0732	0.0159	0.6571	-0.0458	0.5873	93.1506	85.7728	208.132
58	3.9909	-1.6457	0.9909	2.5457	-0.2429	4.6372	-0.1414	0.0593	0.3045	-0.1325	-0.0521	-0.024	-0.1599	-0.3094	0.0923	0.9419	0.1069	0.0114	0.7033	-0.117	0.8579	77.744	95.5375	200.8356
59	7.2991	0.9664	-0.1919	4.1493	0.7021	2.7592	0.2232	0.5784	-0.5402	-0.6316	0.0431	-0.0756	0.0248	-0.1794	-0.2601	0.9119	0.0294	0.153	0.5544	0.0995	0.7066	81.1124	85.9812	199.7907
60	9.3215	-5.2324	6.1519	3.0218	-3.2221	4.1567	-0.4054	-0.637	-0.253	0.9099	0.4056	-0.5167	-0.0591	-0.2086	-0.1237	1.0761	0.138	0.0394	0.5899	-0.1682	0.7772	85.8225	51.3738	199.2927
61	1.1766	-0.4486	-0.0342	2.009	-3.6562	7.2029	-1.1696	-0.9558	-0.2069	1.5703	1.205	-0.9821	-0.8649	-1.311	-0.4719	1.1399	0.2092	-0.3124	0.4682	0.0439	0.7193	89.0009	55.6758	207.7819
62	9.5515	2.2605	-0.1263	6.4481	2.9709	4.6575	-0.3266	-0.4713	1.2227	-0.3101	0.9166	0.2323	0.2483	0.548	-0.3771	0.5489	-0.098	-0.1326	0.5109	0.0277	0.4655	81.1652	55.1492	200.9423
63	2.1655	-1.2826	2.2223	6.5217	-2.8608	3.1351	0.2456	0.9484	0.2186	-0.2293	-0.06	-0.7945	-1.3215	1.8565	-0.2725	0.6561	-0.2031	0.0922	0.9998	0.0393	0.6181	94.617	56.5652	198.1859
64	4.47	-2.0411	4.0222	2.45	-2.221	3.9634	0.3327	0.1943	-0.2103	-0.1949	0.1717	-0.488	1.0262	0.3802	-0.8725	0.775	0.0156	-0.0381	1.0708	-0.351	0.8478	88.3989	49.5764	192.1471
65	2.3337	-1.065	1.574	9.5862	-1.2086	0.9277	0.1977	0.2755	-1.0592	-0.082	0.5951	-0.0966	0.0176	0.4094	-0.5303	0.9485	0.1426	-0.0487	0.783	-0.2207	0.9267	89.4478	41.9516	190.1854
66	4.1088	-3.3944	4.0182	3.0423	-3.4923	3.9232	1.2697	-0.9137	1.0258	-0.4863	-0.7168	1.119	-1.7202	-0.0266	-0.2557	1.0848	-0.1041	0.1986	0.8028	-0.0594	0.7982	86.6366	37.7684	187.577
67	4.6905	3.6519	-2.1018	3.6469	-0.1013	4.9415	-0.0153	-0.2325	0.1348	0.6141	0.3303	0.0509	1.2504	-0.1715	0.2468	0.8476	0.0218	0.0467	0.7171	-0.1207	0.6926	73.4984	46.9284	194.4515
68	8.9572	1.6004	1.1682	4.4601	0.4418	0.8809	0.1713	-0.1182	-0.0254	-0.0061	-0.6674	0.0229	0.4379	-0.4874	0.3183	1.0006	-0.112	0.016	0.4868	0.0102	0.5208	74.5274	41.8155	190.408
69	5.3365	3.7259	-2.7057	2.8756	-1.8465	1.7903	0.1954	-0.145	0.8484	-0.4784	-0.4357	1.3175	0.1142	-0.1837	0.3687	0.9746	0.087	-0.1273	0.5675	-0.1626	0.6648	68.1486	51.339	192.4639
70	2.6966	2.5713	-2.0648	2.1893	-1.7805	6.4205	-0.024	-0.7074	0.4941	-0.1655	-0.2992	0.954	-0.4598	0.4313	0.0089	0.6632	0.028	0.074	0.9813	-0.2554	0.9491	69.6594	56.0752	203.3725
71	2.6601	1.6558	-1.7939	4.6603	-4.5139	6.0382	1.5391	0.4888	0.5389	-1.0789	1.5924	-0.3366	-0.0518	2.1113	0.9759	1.0424	0.5005	-0.3313	1.1762	-0.298	0.8734	72.5602	58.0799	208.0702
72	7.6752	-2.7315	-6.0106	0.8851	1.9404	9.5875	-0.3029	0.344	-0.5885	-2.8765	-0.509	0.7412	1.9328	-1.1546	0.9771	1.7585	-0.0086	0.0674	0.9998	-0.1606	1.0215	85.0155	35.7605	148.526
73	5.9316	2.1617	-0.3963	3.4793	1.0998	4.0424	0.5546	-0.4948	1.4813	0.076	-0.5508	0.8845	-2.0385	0.809	-0.1052	1.5083	-0.1632	0.2977	0.8254	-0.0481	1.1301	89.2794	53.2917	126.1685
74	6.5654	6.0513	-7.3557	6.1935	-5.5793	7.9061	0.7282	-0.7717	0.6065	0.8364	-0.6165	0.7137	-0.7246	1.2376	0.0197	1.2981	-0.0565	0.0142	1.0824	0.0661	0.8623	107.2538	76.5881	100.8912
75	4.13	2.2269	5.1117	9.059	-0.2276	8.0327	2.1564	-1.0109	-1.5756	1.4978	-1.7763	1.8235	1.0898	0.7996	-0.7125	1.7648	-0.249	0.1597	1.3363	-0.2835	1.1916	129.3294	69.6553	80.4473
76	3.0704	1.3603	-2.2945	2.9176	1.0429	3.5724	-0.5232	0.0869	-0.1865	-3.2626	-0.013	0.0478	1.8902	3.0215	0.9476	1.6271	-0.1852	0.0042	1.2544	-0.1924	1.2875	110.7668	101.6018	82.5224
77	3.5219	3.6039	-2.9627	4.0501	-5.2862	7.8901	1.0778	-1.0875	0.5706	0.8841	-0.2163	0.9866	-0.6684	1.0601	-0.8368	1.306	-0.01	0.2094	0.8962	-0.0989	0.8158	99.8964	69.358	110.8404
78	4.7087	4.5228	3.8119	4.7243	5.1588	5.4437	-0.0178	1.0982	0.6091	0.075	0.7513	-0.0143	-0.815	-0.385	-0.5601	1.3112	-0.0465	0.0472	1.2533	0.0179	1.0982	74.1451	39.3557	123.343
79	8.6025	-7.5931	-0.2357	6.9192	1.8745	9.2024	1.1906	0.5779	0.8993	-0.5748	-0.1002	-2.2585	-1.7229	0.4976	-1.0387	1.3355	-0.0728	-0.0046	1.1363	0.16	1.2172	92.0966	65.6376	246.2287
80	2.8117	2.2201	2.8874	6.5224	5.8585	3.9472	1.0892	-0.6847	-0.7636	0.7259	-0.919	-1.0331	1.0853	-1.2897	-0.3391	1.5394	-0.1571	-0.123	0.8988	0.055	0.9679	81.6143	37.3976	213.8724
81	3.8672	-2.1491	3.4326	7.3972	-7.9193	9.2587	0.071	-0.4039	0.1077	0.7173	-0.3451	0.8549	-0.7512	-0.333	1.0525	1.2822	-0.0776	-0.1174	1.0049	0.0105	0.8954	56.0463	24.9063	183.7781
82	2.6282	1.2704	0.4554	5.8226	6.6814	8.5075	1.5038	0.2418	0.3758	-3.3578	-1.2411	-1.0307	-1.9574	-3.0268	0.1226	1.3961	-0.0283	0.0007	1.2383	0.2746	1.2979	67.007	-0.2654	180.2122
83	3.0474	3.8921	3.9796	4.8896	5.1316	5.1577	0.7537	-0.5169	-0.5529	0.7944	-0.175	-0.9421	0.4594	-0.7472	-0.6403	1.3287	-0.0176	-0.1073	0.9559	0.0473	0.962	81.8277	37.3493	211.8191
84	9.0719	4.6866	2.8333	3.155	1.1867	0.9271	-0.9612	5.2445	-1.2375	-2.7193	0.7635	1.183	5.5014	-1.5661	-0.3947	2.0711	-0.1276	-0.0322	1.5302	-0.0741	1.3415	110.5148	68.1076	214.1369

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID
1	X-RAY DIFFRACTION	1	chain 'D' and (resid 15 through 78 )	D
2	X-RAY DIFFRACTION	2	chain 'D' and (resid 79 through 213 )	D
3	X-RAY DIFFRACTION	3	chain 'D' and (resid 214 through 281 )	D
4	X-RAY DIFFRACTION	4	chain 'C' and (resid 2 through 78 )	C
5	X-RAY DIFFRACTION	5	chain 'C' and (resid 79 through 108 )	C
6	X-RAY DIFFRACTION	6	chain 'C' and (resid 109 through 155 )	C
7	X-RAY DIFFRACTION	7	chain 'C' and (resid 156 through 227 )	C
8	X-RAY DIFFRACTION	8	chain 'C' and (resid 228 through 279 )	C
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 15 through 36 )	A
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 37 through 54 )	A
11	X-RAY DIFFRACTION	11	chain 'A' and (resid 55 through 78 )	A
12	X-RAY DIFFRACTION	12	chain 'A' and (resid 79 through 108 )	A
13	X-RAY DIFFRACTION	13	chain 'A' and (resid 109 through 133 )	A
14	X-RAY DIFFRACTION	14	chain 'A' and (resid 134 through 174 )	A
15	X-RAY DIFFRACTION	15	chain 'A' and (resid 175 through 213 )	A
16	X-RAY DIFFRACTION	16	chain 'A' and (resid 214 through 242 )	A
17	X-RAY DIFFRACTION	17	chain 'A' and (resid 243 through 259 )	A
18	X-RAY DIFFRACTION	18	chain 'A' and (resid 260 through 281 )	A
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 2 through 78 )	B
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 79 through 108 )	B
21	X-RAY DIFFRACTION	21	chain 'B' and (resid 109 through 155 )	B
22	X-RAY DIFFRACTION	22	chain 'B' and (resid 156 through 174 )	B
23	X-RAY DIFFRACTION	23	chain 'B' and (resid 175 through 227 )	B
24	X-RAY DIFFRACTION	24	chain 'B' and (resid 228 through 281 )	B
25	X-RAY DIFFRACTION	25	chain 'F' and (resid 1 through 12 )	F
26	X-RAY DIFFRACTION	26	chain 'F' and (resid 13 through 22 )	F
27	X-RAY DIFFRACTION	27	chain 'F' and (resid 23 through 36 )	F
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 37 through 50 )	F
29	X-RAY DIFFRACTION	29	chain 'F' and (resid 51 through 61 )	F
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 62 through 73 )	F
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 74 through 83 )	F
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 84 through 93 )	F
33	X-RAY DIFFRACTION	33	chain 'E' and (resid 1 through 12 )	E
34	X-RAY DIFFRACTION	34	chain 'E' and (resid 13 through 36 )	E
35	X-RAY DIFFRACTION	35	chain 'E' and (resid 37 through 50 )	E
36	X-RAY DIFFRACTION	36	chain 'E' and (resid 51 through 67 )	E
37	X-RAY DIFFRACTION	37	chain 'E' and (resid 68 through 73 )	E
38	X-RAY DIFFRACTION	38	chain 'E' and (resid 74 through 83 )	E
39	X-RAY DIFFRACTION	39	chain 'E' and (resid 84 through 93 )	E
40	X-RAY DIFFRACTION	40	chain 'L' and (resid 15 through 88 )	L
41	X-RAY DIFFRACTION	41	chain 'L' and (resid 89 through 108 )	L
42	X-RAY DIFFRACTION	42	chain 'L' and (resid 109 through 226 )	L
43	X-RAY DIFFRACTION	43	chain 'L' and (resid 227 through 281 )	L
44	X-RAY DIFFRACTION	44	chain 'K' and (resid 2 through 78 )	K
45	X-RAY DIFFRACTION	45	chain 'K' and (resid 79 through 108 )	K
46	X-RAY DIFFRACTION	46	chain 'K' and (resid 109 through 133 )	K
47	X-RAY DIFFRACTION	47	chain 'K' and (resid 134 through 155 )	K
48	X-RAY DIFFRACTION	48	chain 'K' and (resid 156 through 174 )	K
49	X-RAY DIFFRACTION	49	chain 'K' and (resid 175 through 213 )	K
50	X-RAY DIFFRACTION	50	chain 'K' and (resid 214 through 244 )	K
51	X-RAY DIFFRACTION	51	chain 'K' and (resid 245 through 279 )	K
52	X-RAY DIFFRACTION	52	chain 'I' and (resid 15 through 78 )	I
53	X-RAY DIFFRACTION	53	chain 'I' and (resid 79 through 153 )	I
54	X-RAY DIFFRACTION	54	chain 'I' and (resid 154 through 174 )	I
55	X-RAY DIFFRACTION	55	chain 'I' and (resid 175 through 281 )	I
56	X-RAY DIFFRACTION	56	chain 'J' and (resid 2 through 78 )	J
57	X-RAY DIFFRACTION	57	chain 'J' and (resid 79 through 108 )	J
58	X-RAY DIFFRACTION	58	chain 'J' and (resid 109 through 198 )	J
59	X-RAY DIFFRACTION	59	chain 'J' and (resid 199 through 281 )	J
60	X-RAY DIFFRACTION	60	chain 'N' and (resid 1 through 12 )	N
61	X-RAY DIFFRACTION	61	chain 'N' and (resid 13 through 22 )	N
62	X-RAY DIFFRACTION	62	chain 'N' and (resid 23 through 36 )	N
63	X-RAY DIFFRACTION	63	chain 'N' and (resid 37 through 50 )	N
64	X-RAY DIFFRACTION	64	chain 'N' and (resid 51 through 67 )	N
65	X-RAY DIFFRACTION	65	chain 'N' and (resid 68 through 85 )	N
66	X-RAY DIFFRACTION	66	chain 'N' and (resid 86 through 92 )	N
67	X-RAY DIFFRACTION	67	chain 'M' and (resid 1 through 12 )	M
68	X-RAY DIFFRACTION	68	chain 'M' and (resid 13 through 36 )	M
69	X-RAY DIFFRACTION	69	chain 'M' and (resid 37 through 67 )	M
70	X-RAY DIFFRACTION	70	chain 'M' and (resid 68 through 85 )	M
71	X-RAY DIFFRACTION	71	chain 'M' and (resid 86 through 92 )	M
72	X-RAY DIFFRACTION	72	chain 'G' and (resid 2 through 6 )	G
73	X-RAY DIFFRACTION	73	chain 'G' and (resid 7 through 16 )	G
74	X-RAY DIFFRACTION	74	chain 'G' and (resid 17 through 31 )	G
75	X-RAY DIFFRACTION	75	chain 'G' and (resid 32 through 34 )	G
76	X-RAY DIFFRACTION	76	chain 'H' and (resid 1 through 5 )	H
77	X-RAY DIFFRACTION	77	chain 'H' and (resid 6 through 25 )	H
78	X-RAY DIFFRACTION	78	chain 'H' and (resid 26 through 33 )	H
79	X-RAY DIFFRACTION	79	chain 'O' and (resid 2 through 6 )	O
80	X-RAY DIFFRACTION	80	chain 'O' and (resid 7 through 26 )	O
81	X-RAY DIFFRACTION	81	chain 'O' and (resid 27 through 34 )	O
82	X-RAY DIFFRACTION	82	chain 'P' and (resid 1 through 5 )	P
83	X-RAY DIFFRACTION	83	chain 'P' and (resid 6 through 30 )	P
84	X-RAY DIFFRACTION	84	chain 'P' and (resid 31 through 33 )	P

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