+Open data
-Basic information
Entry | Database: PDB / ID: 5cbe | ||||||
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Title | E10 in complex with CXCL13 | ||||||
Components |
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Keywords | IMMUNE SYSTEM / anti-CXCL13 / E10 / scFv | ||||||
Function / homology | Function and homology information lymphocyte chemotaxis across high endothelial venule / CXCR5 chemokine receptor binding / endothelial cell chemotaxis to fibroblast growth factor / negative regulation of endothelial cell chemotaxis to fibroblast growth factor / CCR10 chemokine receptor binding / B cell chemotaxis / CXCR3 chemokine receptor binding / regulation of humoral immune response / chronic inflammatory response / positive regulation of cell-cell adhesion mediated by integrin ...lymphocyte chemotaxis across high endothelial venule / CXCR5 chemokine receptor binding / endothelial cell chemotaxis to fibroblast growth factor / negative regulation of endothelial cell chemotaxis to fibroblast growth factor / CCR10 chemokine receptor binding / B cell chemotaxis / CXCR3 chemokine receptor binding / regulation of humoral immune response / chronic inflammatory response / positive regulation of cell-cell adhesion mediated by integrin / positive regulation of T cell chemotaxis / CXCR chemokine receptor binding / positive regulation of integrin activation / germinal center formation / CD22 mediated BCR regulation / activation of GTPase activity / chemokine-mediated signaling pathway / Chemokine receptors bind chemokines / chemokine activity / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / fibroblast growth factor binding / immunoglobulin complex / immunoglobulin mediated immune response / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / regulation of angiogenesis / Scavenging of heme from plasma / antigen binding / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / neutrophil chemotaxis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCGR3A-mediated phagocytosis / FCERI mediated MAPK activation / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / antimicrobial humoral immune response mediated by antimicrobial peptide / cell-cell signaling / heparin binding / positive regulation of cytosolic calcium ion concentration / G alpha (i) signalling events / cellular response to lipopolysaccharide / Potential therapeutics for SARS / adaptive immune response / receptor ligand activity / cell surface receptor signaling pathway / defense response to bacterium / immune response / inflammatory response / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Tu, C. / Bard, J. / Mosyak, L. | ||||||
Citation | Journal: J. Biol. Chem. / Year: 2016 Title: A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv). Authors: Tu, C. / Terraube, V. / Tam, A.S. / Stochaj, W. / Fennell, B.J. / Lin, L. / Stahl, M. / LaVallie, E.R. / Somers, W. / Finlay, W.J. / Mosyak, L. / Bard, J. / Cunningham, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5cbe.cif.gz | 252.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5cbe.ent.gz | 205.2 KB | Display | PDB format |
PDBx/mmJSON format | 5cbe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cb/5cbe ftp://data.pdbj.org/pub/pdb/validation_reports/cb/5cbe | HTTPS FTP |
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-Related structure data
Related structure data | 5cbaC 2r3zS 3gv3S 3il8S 4hsvS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | Trimer confirmed by gel filtration |
-Components
#1: Antibody | Mass: 14663.956 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line: HEK293 / Gene: IGHV1-69-2 / Cell line (production host): HEK293F / Production host: Mammalia (mammals) / References: UniProt: A0A0B4J2H0 #2: Antibody | Mass: 12844.972 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293F / Production host: Mammalia (mammals) / References: UniProt: P04209 #3: Protein | Mass: 10466.571 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CXCL13, BCA1, BLC, SCYB13 / Production host: Escherichia coli (E. coli) / References: UniProt: O43927 #4: Chemical | ChemComp-EDO / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57 % / Description: long rod |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 100 mM KH2PO4, 100 mM NaH2PO4, 100 mM MES pH 6, 2000 mM NaCl PH range: 6 |
-Data collection
Diffraction | Mean temperature: 193 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 10, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 34874 / % possible obs: 100 % / Redundancy: 11.6 % / Biso Wilson estimate: 59.02 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 30.4 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 9.1 % / Rmerge(I) obs: 0.605 / Mean I/σ(I) obs: 3.6 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: E10 and 3GV3/2R3Z/4HSV/3IL8 Resolution: 2.4→40.92 Å / Cor.coef. Fo:Fc: 0.9049 / Cor.coef. Fo:Fc free: 0.8838 / SU R Cruickshank DPI: 0.322 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.316 / SU Rfree Blow DPI: 0.237 / SU Rfree Cruickshank DPI: 0.242
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Displacement parameters | Biso mean: 82.67 Å2
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Refine analyze | Luzzati coordinate error obs: 0.609 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.4→40.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.47 Å / Total num. of bins used: 17
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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