- PDB-4zws: Crystal Structure of the Bacteriophage T4 recombination mediator ... -
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Basic information
Entry
Database: PDB / ID: 4zws
Title
Crystal Structure of the Bacteriophage T4 recombination mediator protein UvsY, Lattice Type III
Components
Recombination protein uvsYGenetic recombination
Keywords
VIRAL PROTEIN / recombination / DNA binding / homo-heptamer / asymmetry / alpha barrel
Function / homology
Recombination, repair and ssDNA binding protein UvsY / Recombination, repair and ssDNA binding protein UvsY / DNA biosynthetic process / DNA recombination / DNA binding / Recombination protein uvsY
A: Recombination protein uvsY B: Recombination protein uvsY C: Recombination protein uvsY D: Recombination protein uvsY E: Recombination protein uvsY F: Recombination protein uvsY G: Recombination protein uvsY
Mass: 15865.247 Da / Num. of mol.: 7 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Gene: uvsY / Plasmid: pET21 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P04537
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 3.3 Å3/Da / Density % sol: 62.72 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 1:1 UvsY (10 mg/mL, 150 mM sodium chloride, 20 mM HEPES, pH 7.5, 2 mM DTT) to 10% Jeffamine T-403, 10% Jeffamine ED-2003, 100 mM Tris-HCl, pH 8.0 in the presence of 750 uM dA3 oligonucleotide