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- PDB-4uhp: Crystal structure of the pyocin AP41 DNase-Immunity complex -

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Basic information

Entry
Database: PDB / ID: 4uhp
TitleCrystal structure of the pyocin AP41 DNase-Immunity complex
Components
  • BACTERIOCIN IMMUNITY PROTEIN
  • LARGE COMPONENT OF PYOCIN AP41
KeywordsHYDROLASE / BACTERIOCIN / DNASE / PYOCIN / DNASE-IM
Function / homology
Function and homology information


bacteriocin immunity / cytolysis / toxic substance binding / endonuclease activity / killing of cells of another organism / defense response to bacterium / signaling receptor binding
Similarity search - Function
Pyocin S killer protein / S-type Pyocin / Colicin E7 immunity protein; Chain B, fragment: Endonuclease domain / Colicin/pyocin, DNase domain / Colicin E immunity protein / Colicin immunity protein/pyocin immunity protein / Colicin E immunity protein superfamily / Colicin immunity protein / pyocin immunity protein / Colicin/pyocin, DNase domain superfamily / Pyosin/cloacin translocation domain ...Pyocin S killer protein / S-type Pyocin / Colicin E7 immunity protein; Chain B, fragment: Endonuclease domain / Colicin/pyocin, DNase domain / Colicin E immunity protein / Colicin immunity protein/pyocin immunity protein / Colicin E immunity protein superfamily / Colicin immunity protein / pyocin immunity protein / Colicin/pyocin, DNase domain superfamily / Pyosin/cloacin translocation domain / Pyosin/cloacin translocation domain superfamily / HNH nucleases / His-Me finger superfamily / HNH nuclease / Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A / Alpha-Beta Complex / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Large component of pyocin AP41 / Bacteriocin immunity protein
Similarity search - Component
Biological speciesPSEUDOMONAS AERUGINOSA PAO1 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsJoshi, A. / Chen, S. / Wojdyla, J.A. / Kaminska, R. / Kleanthous, C.
CitationJournal: J.Mol.Biol. / Year: 2015
Title: Structures of the Ultra-High Affinity Protein-Protein Complexes of Pyocins S2 and Ap41 and Their Cognate Immunity Proteins from Pseudomonas Aeruginosa
Authors: Joshi, A. / Grinter, R. / Josts, I. / Chen, S. / Wojdyla, J.A. / Lowe, E.D. / Kaminska, R. / Sharp, C. / Mccaughey, L. / Roszak, A.W. / Cogdell, R.J. / Byron, O. / Walker, D. / Kleanthous, C.
History
DepositionMar 25, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 5, 2015Provider: repository / Type: Initial release
Revision 1.1Sep 2, 2015Group: Database references
Revision 1.2Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: LARGE COMPONENT OF PYOCIN AP41
B: BACTERIOCIN IMMUNITY PROTEIN
C: LARGE COMPONENT OF PYOCIN AP41
D: BACTERIOCIN IMMUNITY PROTEIN
E: LARGE COMPONENT OF PYOCIN AP41
F: BACTERIOCIN IMMUNITY PROTEIN
G: LARGE COMPONENT OF PYOCIN AP41
H: BACTERIOCIN IMMUNITY PROTEIN


Theoretical massNumber of molelcules
Total (without water)106,0888
Polymers106,0888
Non-polymers00
Water10,737596
1
C: LARGE COMPONENT OF PYOCIN AP41
D: BACTERIOCIN IMMUNITY PROTEIN


Theoretical massNumber of molelcules
Total (without water)26,5222
Polymers26,5222
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1770 Å2
ΔGint-6.3 kcal/mol
Surface area10570 Å2
MethodPISA
2
G: LARGE COMPONENT OF PYOCIN AP41
H: BACTERIOCIN IMMUNITY PROTEIN


Theoretical massNumber of molelcules
Total (without water)26,5222
Polymers26,5222
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1730 Å2
ΔGint-7.2 kcal/mol
Surface area11100 Å2
MethodPISA
3
A: LARGE COMPONENT OF PYOCIN AP41
B: BACTERIOCIN IMMUNITY PROTEIN


Theoretical massNumber of molelcules
Total (without water)26,5222
Polymers26,5222
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1740 Å2
ΔGint-7.2 kcal/mol
Surface area11540 Å2
MethodPISA
4
E: LARGE COMPONENT OF PYOCIN AP41
F: BACTERIOCIN IMMUNITY PROTEIN


Theoretical massNumber of molelcules
Total (without water)26,5222
Polymers26,5222
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1720 Å2
ΔGint-7 kcal/mol
Surface area11610 Å2
MethodPISA
Unit cell
Length a, b, c (Å)36.604, 75.627, 83.171
Angle α, β, γ (deg.)79.19, 78.05, 77.00
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
LARGE COMPONENT OF PYOCIN AP41 / PYOCIN AP41 LARGE COMPONENT


Mass: 15153.019 Da / Num. of mol.: 4 / Fragment: DNASE DOMAIN, RESIDUES 642-777
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) PSEUDOMONAS AERUGINOSA PAO1 (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q51502
#2: Protein
BACTERIOCIN IMMUNITY PROTEIN / PYOCIN AP41 SMALL COMPONENT


Mass: 11368.884 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) PSEUDOMONAS AERUGINOSA PAO1 (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q51503
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 596 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.05 Å3/Da / Density % sol: 42 % / Description: NONE
Crystal growDetails: 40% JEFFAMINE D2000, 0.1 M HEPES PH 6.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9795
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 16, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 2→40.24 Å / Num. obs: 55279 / % possible obs: 97.2 % / Observed criterion σ(I): 6 / Redundancy: 1.8 % / Biso Wilson estimate: 22.46 Å2 / Rmerge(I) obs: 0.18 / Net I/σ(I): 4.7
Reflection shellResolution: 2→2.05 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.94 / Mean I/σ(I) obs: 2.7 / % possible all: 96.2

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1BX1
Resolution: 2→40.236 Å / SU ML: 0.26 / σ(F): 1.96 / Phase error: 25.11 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2347 2682 4.8 %
Rwork0.1858 --
obs0.1882 55266 97.22 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2→40.236 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6983 0 0 596 7579
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0037167
X-RAY DIFFRACTIONf_angle_d0.6679676
X-RAY DIFFRACTIONf_dihedral_angle_d11.3692674
X-RAY DIFFRACTIONf_chiral_restr0.0281002
X-RAY DIFFRACTIONf_plane_restr0.0031275
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2-2.03640.32231610.25772787X-RAY DIFFRACTION96
2.0364-2.07550.30861240.24452692X-RAY DIFFRACTION96
2.0755-2.11790.29461360.24232780X-RAY DIFFRACTION97
2.1179-2.16390.32531550.23542743X-RAY DIFFRACTION96
2.1639-2.21430.25851550.22092661X-RAY DIFFRACTION96
2.2143-2.26960.27681340.22142790X-RAY DIFFRACTION96
2.2696-2.3310.29311330.21322744X-RAY DIFFRACTION97
2.331-2.39960.27781390.21262767X-RAY DIFFRACTION97
2.3996-2.4770.28931380.21342745X-RAY DIFFRACTION97
2.477-2.56560.26541550.20632753X-RAY DIFFRACTION98
2.5656-2.66830.24781360.19712778X-RAY DIFFRACTION97
2.6683-2.78970.2351610.18752756X-RAY DIFFRACTION97
2.7897-2.93670.21481260.1932759X-RAY DIFFRACTION98
2.9367-3.12060.25281590.19742775X-RAY DIFFRACTION97
3.1206-3.36150.251530.18672793X-RAY DIFFRACTION98
3.3615-3.69950.21291130.16762837X-RAY DIFFRACTION98
3.6995-4.23440.19641360.15442798X-RAY DIFFRACTION99
4.2344-5.33290.15921300.13972825X-RAY DIFFRACTION99
5.3329-40.24430.17281380.15152801X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
16.7275.7960.36827.79223.04845.5256-0.01620.13190.66750.19290.18180.1367-0.8474-0.0435-0.29020.38130.1017-0.12540.17630.00920.3289-24.780218.9026-39.7738
24.17581.78762.28624.11740.65153.93220.2098-0.1927-0.04530.2801-0.0759-0.07740.2931-0.1851-0.1260.25760.05470.02340.19290.01870.1843-32.49338.557-42.5958
38.34974.9908-1.66583.74250.13244.9647-0.42190.08920.31730.00160.4567-0.1958-0.10250.09030.03310.16110.00960.01630.20090.00090.2426-23.43759.081-47.4319
40.9023-0.75720.47433.60220.84162.414-0.0228-0.07440.0429-0.16240.0036-0.054-0.11680.04160.02630.12070.00550.0130.17140.010.1651-31.35078.4391-51.5921
56.6489-4.5992-1.00874.33590.12771.86920.021-0.02550.0745-0.60320.45790.4772-0.0204-0.317-0.39190.2194-0.0129-0.00140.290.04750.2658-46.35119.2719-58.9277
64.35261.2564-0.56796.39652.13140.94750.07840.58190.0345-0.89120.7453-0.4957-0.3247-0.4282-0.61530.5698-0.16360.13250.82170.06040.4221-17.8792-2.031-75.9146
77.2059-1.2266-0.14434.41571.14132.2763-0.20810.4369-0.2796-0.6210.2506-0.62330.27470.6090.0070.34550.01750.09350.3345-0.02960.3249-19.8922-8.0954-70.4369
84.83986.9345-1.81989.9582-2.73573.63290.33360.727-0.35010.4486-0.036-1.0672-0.13660.4591-0.22790.28860.07570.02080.33680.00270.3738-14.8963-1.4754-58.3416
96.30225.41924.22427.67395.25534.0636-0.29240.13610.3671-0.751-0.0949-0.0062-0.8868-0.13460.31570.3370.01310.08680.26980.05840.251-22.5673.6426-66.3005
102.561.09780.46782.04620.71157.20380.10120.1633-0.1896-0.5884-0.10820.04190.0968-0.2693-0.02220.3010.0718-0.04760.1987-0.05780.2033-33.0286-6.7304-63.3335
112.13790.61491.59443.91310.92674.25050.01180.2139-0.453-0.28250.09770.0010.58680.2255-0.05010.46680.0790.0160.2598-0.02040.2332-29.1724-12.2591-73.6937
125.370.5108-3.82521.14680.24645.85090.58180.25880.7494-1.24360.4350.71320.6951-0.12610.86510.95910.0831-0.30870.2470.07610.3845-25.566510.0209-20.3263
137.39662.4691-4.24026.4096-4.59876.38640.502-0.23890.0496-0.6250.20250.69530.5327-0.6433-0.62760.4755-0.1577-0.14220.40770.04550.2734-26.194810.6016-20.4875
142.2432-0.7891-2.28552.72583.75015.96220.35430.49440.0451-0.6321-0.0099-0.36380.93760.9997-0.34950.50040.087-0.00040.4303-0.0320.1993-14.191413.1768-21.6332
152.286-0.6032-1.6325.3196-2.0044.99680.10950.1103-0.0151-0.06730.09030.2581-0.2765-0.1027-0.19090.22270.0039-0.03950.2094-0.040.2137-22.340622.0104-11.8114
160.384-0.2885-0.28731.8761.28830.97180.151-0.1132-0.415-0.49050.37940.34771.55550.18240.20721.14310.0102-0.34260.1894-0.14760.199-19.56335.3823-12.7149
176.3755-4.40351.92799.31692.14966.7836-0.2945-0.0269-0.6183-0.13570.26651.23160.1459-0.0890.09780.2827-0.00480.00830.23820.07170.3011-25.44012.0531-3.101
183.05423.12911.5345.22694.4196.98690.3584-0.0996-0.10730.11280.1579-0.3979-0.02111.1628-0.41420.2832-0.07280.050.3062-0.03930.246-10.895433.79012.718
196.1887-1.06520.4535.5762-2.57661.36480.7584-0.20540.4439-0.6605-0.313-1.102-0.57470.7256-0.39660.4017-0.1080.10940.4339-0.10430.3394-8.61129.2097-11.9631
207.3564-1.3586-4.54366.52833.04924.51040.1358-0.368-0.5210.09150.1798-0.64530.83560.7513-0.29580.29090.02-0.05710.3281-0.05180.2823-10.246622.4789-1.9574
212.85541.23350.11392.46913.2999.06220.0992-0.20460.17690.54650.0574-0.3845-0.10050.0692-0.10450.29710.0276-0.02420.1652-0.02270.1619-21.976724.27692.2565
223.7193-3.3767-0.96447.51215.0954.38420.22290.36990.05490.0131-0.41570.1807-0.3193-0.60370.15340.28280.02440.02270.2877-0.00060.1968-27.392330.79-3.4719
230.11730.32880.05153.8712-1.03515.90050.3731-0.00690.3810.2850.15710.0788-0.63210.1078-0.390.2546-0.01520.04670.1943-0.03360.273-20.063134.88868.0452
241.94332.8012-0.37384.43860.98775.8686-0.1573-0.282-0.67970.03790.0994-0.37490.86650.5781-0.08080.29640.0826-0.01830.2393-0.06120.2276-0.577534.8226-65.5558
251.60180.7188-1.43072.80151.95834.3190.10670.04050.1613-0.5788-0.07520.7076-0.2628-0.5790.00730.22840.1026-0.03240.32360.01830.3069-16.332743.5227-54.7753
266.23894.3924-3.79134.9817-5.11346.39950.141-0.396-0.0523-0.1026-0.3668-0.13730.758-0.49280.20030.3254-0.0187-0.05470.2456-0.040.1947-14.039637.9361-53.393
277.6449-0.9752-0.45.15752.85115.69750.08170.10010.0956-0.13310.084-0.20120.07260.2771-0.15240.17240.00550.03460.13180.00390.12-2.149844.0125-62.761
282.92910.21330.8830.4639-0.4564.8368-0.31250.0496-0.5894-0.25130.12550.6263-0.3154-0.5411-0.04720.18590.0539-0.05050.25360.06270.3241-13.114853.4273-56.2545
291.08170.7842-0.02723.04731.1661.98420.0031-0.0255-0.17340.17660.0719-0.22220.1677-0.0018-0.07810.12260.0286-0.0110.20420.00030.1722-3.984847.4964-47.8918
302.36420.1108-0.72252.9835-0.24150.2345-0.102-0.0586-0.3036-0.0082-0.0568-0.07280.83720.42180.12370.3350.0917-0.01390.216-0.01180.1942-4.862431.7006-57.3615
316.05395.2951-1.04459.02620.01225.0701-0.09440.0399-0.4808-0.17380.00740.44970.09290.06780.10530.20920.0422-0.04730.2809-0.01070.3101-8.980330.554-41.7884
322.0118-0.42564.22578.8825-2.76263.04730.15110.5473-0.5632-0.11710.0149-1.2033-0.33861.3311-0.12140.336-0.0399-0.03040.373-0.05560.38059.623562.8184-33.1081
337.38162.91471.89914.25521.80114.87710.09430.0710.2132-0.2621-0.0260.0054-0.42910.746-0.00870.1764-0.03160.01690.22530.00220.1864.130164.929-42.4709
344.5544-4.6044-4.43348.63211.53496.50050.48180.05020.7255-0.1338-0.4599-0.8784-0.62430.9536-0.1180.13830.0018-0.02440.32510.01420.17696.58659.7687-53.0196
355.9004-2.5666-4.80472.49492.97775.245-0.37070.0096-0.51180.29630.02010.06751.06880.43070.21580.18050.05670.00190.21490.0020.21286.201652.8526-42.9205
361.9868-0.2451.31951.87540.02854.2334-0.0893-0.1940.22820.34530.0686-0.0664-0.2219-0.21630.01990.14990.04320.02210.1631-0.00790.1616-6.558660.7833-37.1953
373.45012.005-1.87296.8636-1.81572.95350.17360.77730.17380.35880.1593-0.4478-0.4593-0.0022-0.44520.3231-0.01010.06080.4316-0.03260.27714.948371.8973-34.0782
384.0031-3.2233-0.06162.6480.55878.5034-0.488-0.3761-0.17170.20060.03510.1805-0.69260.30790.21580.40280.0794-0.03990.20870.01880.1876-30.182755.436-0.4097
391.6174-1.97170.16184.0907-0.71163.9476-0.0483-0.2727-0.31020.066-0.0011-0.1025-0.49470.11550.0260.2069-0.03150.05020.3019-0.01660.1693-30.045253.1249-3.3982
404.16562.8112.03582.72491.45884.2188-0.04350.04780.00790.27180.1346-0.0091-0.04430.1178-0.05840.16020.02570.01970.2340.00240.1919-25.853348.1075-8.0276
415.0975-1.76061.0692.17811.20094.1663-0.037-0.29660.08790.32870.15180.15620.38360.0758-0.14510.118-0.01120.03480.18740.02960.187-28.831943.9643-16.1787
420.1287-0.3727-0.67924.24862.56183.66630.0864-0.0040.0189-0.7974-0.0532-0.2001-0.16610.1508-0.14240.20730.0594-0.03620.2883-0.01810.2103-34.743150.9422-21.8885
430.95362.2780.42425.79440.94170.709-0.0196-0.03260.283-0.22570.02160.0763-1.2561-0.24650.02570.50180.0220.03780.2078-0.04310.2325-32.684261.9707-7.0027
447.8453-2.6450.03453.784-2.30452.79510.33190.12750.42590.58090.57320.5111-1.0912-0.951-0.58830.51040.16280.05930.41870.08350.287-44.817759.997-17.172
453.7237-1.06230.59131.54222.16685.6229-0.29780.2869-0.0187-0.16530.4047-0.5146-0.42551.0281-0.05180.172-0.04680.05130.3353-0.02930.2988-21.425435.9692-32.6932
466.02154.4903-1.55746.5332-3.58272.2392-0.10050.1845-0.1456-0.05750.0671-1.14180.34950.2872-0.21610.21260.06130.01820.3792-0.18820.3901-15.636741.6089-20.187
477.36894.53763.93946.46023.98413.3531-0.1931-0.03770.2635-0.93160.0242-0.313-0.79380.09480.22770.198-0.01870.06870.25640.01850.2024-24.139946.3331-27.3679
482.6038-0.04851.85423.07811.06075.4220.2056-0.0242-0.19480.17310.01690.20490.7415-0.0614-0.19540.1575-0.0166-0.00480.1350.00850.1688-33.385633.636-26.2415
492.12-3.8675-0.27267.27470.55195.60750.26570.25340.3693-0.2396-0.1508-0.53240.0890.5544-0.10540.24220.0049-0.00610.2015-0.06870.2471-27.229729.2447-38.8415
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'A' AND (RESID 642 THROUGH 651 )
2X-RAY DIFFRACTION2CHAIN 'A' AND (RESID 652 THROUGH 685 )
3X-RAY DIFFRACTION3CHAIN 'A' AND (RESID 686 THROUGH 699 )
4X-RAY DIFFRACTION4CHAIN 'A' AND (RESID 700 THROUGH 759 )
5X-RAY DIFFRACTION5CHAIN 'A' AND (RESID 760 THROUGH 776 )
6X-RAY DIFFRACTION6CHAIN 'B' AND (RESID 1 THROUGH 6 )
7X-RAY DIFFRACTION7CHAIN 'B' AND (RESID 7 THROUGH 22 )
8X-RAY DIFFRACTION8CHAIN 'B' AND (RESID 23 THROUGH 30 )
9X-RAY DIFFRACTION9CHAIN 'B' AND (RESID 31 THROUGH 49 )
10X-RAY DIFFRACTION10CHAIN 'B' AND (RESID 50 THROUGH 67 )
11X-RAY DIFFRACTION11CHAIN 'B' AND (RESID 68 THROUGH 90 )
12X-RAY DIFFRACTION12CHAIN 'C' AND (RESID 643 THROUGH 663 )
13X-RAY DIFFRACTION13CHAIN 'C' AND (RESID 664 THROUGH 678 )
14X-RAY DIFFRACTION14CHAIN 'C' AND (RESID 679 THROUGH 699 )
15X-RAY DIFFRACTION15CHAIN 'C' AND (RESID 700 THROUGH 727 )
16X-RAY DIFFRACTION16CHAIN 'C' AND (RESID 728 THROUGH 759 )
17X-RAY DIFFRACTION17CHAIN 'C' AND (RESID 760 THROUGH 770 )
18X-RAY DIFFRACTION18CHAIN 'D' AND (RESID 4 THROUGH 22 )
19X-RAY DIFFRACTION19CHAIN 'D' AND (RESID 23 THROUGH 30 )
20X-RAY DIFFRACTION20CHAIN 'D' AND (RESID 31 THROUGH 49 )
21X-RAY DIFFRACTION21CHAIN 'D' AND (RESID 50 THROUGH 59 )
22X-RAY DIFFRACTION22CHAIN 'D' AND (RESID 60 THROUGH 67 )
23X-RAY DIFFRACTION23CHAIN 'D' AND (RESID 68 THROUGH 90 )
24X-RAY DIFFRACTION24CHAIN 'E' AND (RESID 642 THROUGH 651 )
25X-RAY DIFFRACTION25CHAIN 'E' AND (RESID 652 THROUGH 663 )
26X-RAY DIFFRACTION26CHAIN 'E' AND (RESID 664 THROUGH 671 )
27X-RAY DIFFRACTION27CHAIN 'E' AND (RESID 672 THROUGH 699 )
28X-RAY DIFFRACTION28CHAIN 'E' AND (RESID 700 THROUGH 708 )
29X-RAY DIFFRACTION29CHAIN 'E' AND (RESID 709 THROUGH 739 )
30X-RAY DIFFRACTION30CHAIN 'E' AND (RESID 740 THROUGH 759 )
31X-RAY DIFFRACTION31CHAIN 'E' AND (RESID 760 THROUGH 773 )
32X-RAY DIFFRACTION32CHAIN 'F' AND (RESID 3 THROUGH 11 )
33X-RAY DIFFRACTION33CHAIN 'F' AND (RESID 12 THROUGH 22 )
34X-RAY DIFFRACTION34CHAIN 'F' AND (RESID 23 THROUGH 30 )
35X-RAY DIFFRACTION35CHAIN 'F' AND (RESID 31 THROUGH 49 )
36X-RAY DIFFRACTION36CHAIN 'F' AND (RESID 50 THROUGH 81 )
37X-RAY DIFFRACTION37CHAIN 'F' AND (RESID 82 THROUGH 96 )
38X-RAY DIFFRACTION38CHAIN 'G' AND (RESID 643 THROUGH 657 )
39X-RAY DIFFRACTION39CHAIN 'G' AND (RESID 658 THROUGH 685 )
40X-RAY DIFFRACTION40CHAIN 'G' AND (RESID 686 THROUGH 708 )
41X-RAY DIFFRACTION41CHAIN 'G' AND (RESID 709 THROUGH 727 )
42X-RAY DIFFRACTION42CHAIN 'G' AND (RESID 728 THROUGH 735 )
43X-RAY DIFFRACTION43CHAIN 'G' AND (RESID 736 THROUGH 759 )
44X-RAY DIFFRACTION44CHAIN 'G' AND (RESID 760 THROUGH 772 )
45X-RAY DIFFRACTION45CHAIN 'H' AND (RESID 1 THROUGH 22 )
46X-RAY DIFFRACTION46CHAIN 'H' AND (RESID 23 THROUGH 30 )
47X-RAY DIFFRACTION47CHAIN 'H' AND (RESID 31 THROUGH 49 )
48X-RAY DIFFRACTION48CHAIN 'H' AND (RESID 50 THROUGH 81 )
49X-RAY DIFFRACTION49CHAIN 'H' AND (RESID 82 THROUGH 92 )

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