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Yorodumi- PDB-4twk: Crystal structure of human two pore domain potassium ion channel ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4twk | ||||||
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Title | Crystal structure of human two pore domain potassium ion channel TREK1 (K2P2.1) | ||||||
Components | Potassium channel subfamily K member 2 | ||||||
Keywords | TRANSPORT PROTEIN / Ion channel / Membrane protein / K2P | ||||||
Function / homology | Function and homology information TWIK related potassium channel (TREK) / : / Phase 4 - resting membrane potential / positive regulation of cellular response to hypoxia / cardiac ventricle development / stabilization of membrane potential / potassium ion leak channel activity / negative regulation of cardiac muscle cell proliferation / astrocyte projection / potassium channel inhibitor activity ...TWIK related potassium channel (TREK) / : / Phase 4 - resting membrane potential / positive regulation of cellular response to hypoxia / cardiac ventricle development / stabilization of membrane potential / potassium ion leak channel activity / negative regulation of cardiac muscle cell proliferation / astrocyte projection / potassium channel inhibitor activity / outward rectifier potassium channel activity / negative regulation of DNA biosynthetic process / cochlea development / plasma membrane => GO:0005886 / calyx of Held / response to axon injury / voltage-gated potassium channel complex / response to mechanical stimulus / potassium ion transmembrane transport / memory / cellular response to hypoxia / G protein-coupled receptor signaling pathway / apical plasma membrane / neuronal cell body / endoplasmic reticulum membrane / cell surface / endoplasmic reticulum / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Pike, A.C.W. / Dong, Y.Y. / Tessitore, A. / Goubin, S. / Strain-Damerell, C. / Mukhopadhyay, S. / Kupinska, K. / Wang, D. / Chalk, R. / Berridge, G. ...Pike, A.C.W. / Dong, Y.Y. / Tessitore, A. / Goubin, S. / Strain-Damerell, C. / Mukhopadhyay, S. / Kupinska, K. / Wang, D. / Chalk, R. / Berridge, G. / Grieben, M. / Shrestha, L. / Ang, J.H. / Mackenzie, A. / Quigley, A. / Bushell, S.R. / Shintre, C.A. / Faust, B. / Chu, A. / Dong, L. / von Delft, F. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Burgess-Brown, N.A. / Carpenter, E.P. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of human two pore domain potassium ion channel TREK1 (K2P2.1) Authors: Pike, A.C.W. / Dong, Y.Y. / Tessitore, A. / Goubin, S. / Strain-Damerell, C. / Mukhopadhyay, S. / Kupinska, K. / Wang, D. / Chalk, R. / Berridge, G. / Grieben, M. / Shrestha, L. / Ang, J.H. ...Authors: Pike, A.C.W. / Dong, Y.Y. / Tessitore, A. / Goubin, S. / Strain-Damerell, C. / Mukhopadhyay, S. / Kupinska, K. / Wang, D. / Chalk, R. / Berridge, G. / Grieben, M. / Shrestha, L. / Ang, J.H. / Mackenzie, A. / Quigley, A. / Bushell, S.R. / Shintre, C.A. / Faust, B. / Chu, A. / Dong, L. / von Delft, F. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Burgess-Brown, N.A. / Carpenter, E.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4twk.cif.gz | 215.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4twk.ent.gz | 171.3 KB | Display | PDB format |
PDBx/mmJSON format | 4twk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tw/4twk ftp://data.pdbj.org/pub/pdb/validation_reports/tw/4twk | HTTPS FTP |
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-Related structure data
Related structure data | 4bw5S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / Sugars , 2 types, 3 molecules AB
#1: Protein | Mass: 31229.432 Da / Num. of mol.: 2 / Fragment: UNP residues 26-300 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCNK2, TREK, TREK1 / Plasmid: pFB-CT10HF-LIC / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: O95069 #5: Sugar | ChemComp-NAG / | |
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-Non-polymers , 4 types, 36 molecules
#2: Chemical | #3: Chemical | ChemComp-37X / #4: Chemical | ChemComp-MG / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.05 Å3/Da / Density % sol: 69.68 % / Description: Rods |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.25M magnesium formate, 0.1M Sodium Cacodylate pH 6.5, 17% PEG3000, 5% PEG400 PH range: 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9686 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Dec 7, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→39.65 Å / Num. all: 31773 / Num. obs: 31773 / % possible obs: 99.6 % / Redundancy: 7.3 % / Biso Wilson estimate: 74.16 Å2 / Rmerge(I) obs: 0.073 / Net I/σ(I): 15.5 |
Reflection shell | Resolution: 2.6→2.67 Å / Redundancy: 6.3 % / Rmerge(I) obs: 1.836 / Mean I/σ(I) obs: 1.1 / % possible all: 93.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4BW5 Resolution: 2.6→33.94 Å / Cor.coef. Fo:Fc: 0.8906 / Cor.coef. Fo:Fc free: 0.8909 / SU R Cruickshank DPI: 0.298 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.288 / SU Rfree Blow DPI: 0.23 / SU Rfree Cruickshank DPI: 0.237 Details: DIFFRACTION DATA WERE SEVERELY ANISOTROPIC ALL DATA TO 2.6A WAS USED IN REFINEMENT WITHOUT TRUNCATION. NOMINAL RESOLUTION IS 2.8A BASED ON MN (I)/SD(I)>2 CRITERIA
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Displacement parameters | Biso mean: 113.28 Å2
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Refine analyze | Luzzati coordinate error obs: 0.522 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.6→33.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.69 Å / Total num. of bins used: 16
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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