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Yorodumi- PDB-4luo: Fragment-Based Discovery of a Potent Inhibitor of Replication Pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4luo | ||||||
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Title | Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions | ||||||
Components | Replication protein A 70 kDa DNA-binding subunitDNA replication | ||||||
Keywords | PROTEIN BINDING / OB-FOLD / PROTEIN-PROTEIN INTERACTION | ||||||
Function / homology | Function and homology information protein localization to chromosome / DNA replication factor A complex / chromatin-protein adaptor activity / protein localization to site of double-strand break / Removal of the Flap Intermediate / G-rich strand telomeric DNA binding / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / single-stranded telomeric DNA binding / Removal of the Flap Intermediate from the C-strand ...protein localization to chromosome / DNA replication factor A complex / chromatin-protein adaptor activity / protein localization to site of double-strand break / Removal of the Flap Intermediate / G-rich strand telomeric DNA binding / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / single-stranded telomeric DNA binding / Removal of the Flap Intermediate from the C-strand / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / site of DNA damage / Presynaptic phase of homologous DNA pairing and strand exchange / telomere maintenance via telomerase / PCNA-Dependent Long Patch Base Excision Repair / mismatch repair / Activation of the pre-replicative complex / HSF1 activation / Regulation of HSF1-mediated heat shock response / Activation of ATR in response to replication stress / SUMOylation of DNA damage response and repair proteins / telomere maintenance / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / meiotic cell cycle / Recognition of DNA damage by PCNA-containing replication complex / nucleotide-excision repair / Fanconi Anemia Pathway / Termination of translesion DNA synthesis / double-strand break repair via homologous recombination / Translesion Synthesis by POLH / HDR through Homologous Recombination (HRR) / Dual Incision in GG-NER / G2/M DNA damage checkpoint / base-excision repair / DNA-templated DNA replication / PML body / Formation of Incision Complex in GG-NER / Meiotic recombination / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / single-stranded DNA binding / site of double-strand break / Processing of DNA double-strand break ends / DNA recombination / Regulation of TP53 Activity through Phosphorylation / DNA replication / chromosome, telomeric region / damaged DNA binding / DNA repair / DNA damage response / nucleoplasm / metal ion binding / nucleus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.54 Å | ||||||
Authors | Feldkamp, M.D. / Frank, A.O. / Kennedy, J.P. / Waterson, A.G. / Olejniczak, E.T. / Pelz, N.F. / Patrone, J.D. / Vangamudi, B. / Camper, D.V. / Rossanese, O.W. ...Feldkamp, M.D. / Frank, A.O. / Kennedy, J.P. / Waterson, A.G. / Olejniczak, E.T. / Pelz, N.F. / Patrone, J.D. / Vangamudi, B. / Camper, D.V. / Rossanese, O.W. / Fesik, S.W. / Chazin, W.J. | ||||||
Citation | Journal: J.Med.Chem. / Year: 2013 Title: Discovery of a potent inhibitor of replication protein a protein-protein interactions using a fragment-linking approach. Authors: Frank, A.O. / Feldkamp, M.D. / Kennedy, J.P. / Waterson, A.G. / Pelz, N.F. / Patrone, J.D. / Vangamudi, B. / Camper, D.V. / Rossanese, O.W. / Chazin, W.J. / Fesik, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4luo.cif.gz | 92.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4luo.ent.gz | 72.6 KB | Display | PDB format |
PDBx/mmJSON format | 4luo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lu/4luo ftp://data.pdbj.org/pub/pdb/validation_reports/lu/4luo | HTTPS FTP |
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-Related structure data
Related structure data | 4luvC 4luzC 4lw1C 4lwcC 4o0aC 2b29S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13497.728 Da / Num. of mol.: 1 / Fragment: RPA70N (unp residues 1-120) / Mutation: E7R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RPA1, REPA1, RPA70 / Plasmid: PET15B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-DE3 / References: UniProt: P27694 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.26 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 100 MM MES, 200 MM CALCIUM ACETATE, 20% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 0.97872 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 12, 2013 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97872 Å / Relative weight: 1 |
Reflection | Resolution: 1.54→31.211 Å / Num. all: 418133 / Num. obs: 31399 / % possible obs: 99.91 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.111 |
Reflection shell | Resolution: 1.54→1.57 Å / Redundancy: 9.6 % / Rmerge(I) obs: 0.72 / Mean I/σ(I) obs: 5.35 / Num. unique all: 820 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 2B29 Resolution: 1.54→31.211 Å / SU ML: 0.15 / σ(F): 1.33 / Phase error: 19.56 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.54→31.211 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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