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- PDB-4lo7: HA70(D3)-HA17-HA33 -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4lo7
TitleHA70(D3)-HA17-HA33
Components
  • HA-17
  • HA-33
  • HA-70
KeywordsPROTEIN TRANSPORT / progenitor toxin complex / botulinum neurotoxin / botulism / neurotoxin associated protein / hemagglutinin / carbohydrate/sugar binding / secreted protein
Function / homology
Function and homology information


Toxicity of botulinum toxin type A (botA) / Toxicity of botulinum toxin type B (botB) / carbohydrate binding / extracellular region
Similarity search - Function
Hemagglutinin component HA-17 / Jelly Rolls - #1090 / Hemagglutinin component HA-70, C-terminal / Clostridium botulinum HA-17 domain / Haemagglutinin 70 C-terminal domain / Clostridium enterotoxin / Clostridium enterotoxin / Ricin-type beta-trefoil lectin domain-like / Ricin-type beta-trefoil / Lectin domain of ricin B chain profile. ...Hemagglutinin component HA-17 / Jelly Rolls - #1090 / Hemagglutinin component HA-70, C-terminal / Clostridium botulinum HA-17 domain / Haemagglutinin 70 C-terminal domain / Clostridium enterotoxin / Clostridium enterotoxin / Ricin-type beta-trefoil lectin domain-like / Ricin-type beta-trefoil / Lectin domain of ricin B chain profile. / Ricin B, lectin domain / Ricin B-like lectins / Trefoil (Acidic Fibroblast Growth Factor, subunit A) - #50 / Trefoil (Acidic Fibroblast Growth Factor, subunit A) / Trefoil / Jelly Rolls / Sandwich / Mainly Beta
Similarity search - Domain/homology
HA-33 / HA-17 / HA-70
Similarity search - Component
Biological speciesClostridium botulinum (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.73 Å
AuthorsLee, K. / Gu, S. / Jin, L. / Le, T.T. / Cheng, L.W. / Strotmeier, J. / Kruel, A.M. / Yao, G. / Perry, K. / Rummel, A. / Jin, R.
CitationJournal: PLoS Pathog / Year: 2013
Title: Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.
Authors: Kwangkook Lee / Shenyan Gu / Lei Jin / Thi Tuc Nghi Le / Luisa W Cheng / Jasmin Strotmeier / Anna Magdalena Kruel / Guorui Yao / Kay Perry / Andreas Rummel / Rongsheng Jin /
Abstract: Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary ...Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary proteins as progenitor toxin complexes (PTCs) to become highly potent oral poisons. Here, we report the structure of a ∼760 kDa 14-subunit large PTC of serotype A (L-PTC/A) and reveal insight into its absorption mechanism. Using a combination of X-ray crystallography, electron microscopy, and functional studies, we found that L-PTC/A consists of two structurally and functionally independent sub-complexes. A hetero-dimeric 290 kDa complex protects BoNT, while a hetero-dodecameric 470 kDa complex facilitates its absorption in the harsh environment of the gastrointestinal tract. BoNT absorption is mediated by nine glycan-binding sites on the dodecameric sub-complex that forms multivalent interactions with carbohydrate receptors on intestinal epithelial cells. We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry.
History
DepositionJul 12, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 30, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 15, 2017Group: Advisory / Refinement description / Category: pdbx_unobs_or_zero_occ_atoms / software / Item: _software.name
Revision 1.2Feb 28, 2024Group: Advisory / Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_unobs_or_zero_occ_atoms / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HA-70
B: HA-33
C: HA-17
D: HA-33
E: HA-70
F: HA-33
G: HA-17
H: HA-33


Theoretical massNumber of molelcules
Total (without water)228,1598
Polymers228,1598
Non-polymers00
Water0
1
A: HA-70
B: HA-33
C: HA-17
D: HA-33


Theoretical massNumber of molelcules
Total (without water)114,0794
Polymers114,0794
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: HA-70
F: HA-33
G: HA-17
H: HA-33


Theoretical massNumber of molelcules
Total (without water)114,0794
Polymers114,0794
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
E: HA-70


Theoretical massNumber of molelcules
Total (without water)28,8471
Polymers28,8471
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
F: HA-33
G: HA-17
H: HA-33


Theoretical massNumber of molelcules
Total (without water)85,2333
Polymers85,2333
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3940 Å2
ΔGint-23 kcal/mol
Surface area30350 Å2
MethodPISA
5
A: HA-70


Theoretical massNumber of molelcules
Total (without water)28,8471
Polymers28,8471
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
6
B: HA-33
C: HA-17
D: HA-33


Theoretical massNumber of molelcules
Total (without water)85,2333
Polymers85,2333
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4030 Å2
ΔGint-23 kcal/mol
Surface area30260 Å2
MethodPISA
Unit cell
Length a, b, c (Å)159.590, 187.490, 120.430
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212

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Components

#1: Protein HA-70 / HA70 / Non-toxin haemagglutinin HA70


Mass: 28846.920 Da / Num. of mol.: 2 / Fragment: UNP residues 378-626
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridium botulinum (bacteria) / Gene: ha70 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8KHU9
#2: Protein
HA-33 / HA-33 protein / HA34 / Non-toxin haemagglutinin HA34


Mass: 34081.656 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridium botulinum (bacteria) / Gene: HA-33, ha33, ha34 / Production host: Escherichia coli (E. coli) / References: UniProt: Q45871
#3: Protein HA-17 / HA-17 protein / HA17 / Ha17 protein / Non-toxin haemagglutinin HA17


Mass: 17069.195 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridium botulinum (bacteria) / Gene: ha17, HA-17 / Production host: Escherichia coli (E. coli) / References: UniProt: Q45878

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.95 Å3/Da / Density % sol: 68.85 %
Crystal growTemperature: 292 K / Method: evaporation / pH: 8.2
Details: 0.1 M Tris, 0.1 M sodium chloride, 6% PEG2000, pH 8.2, EVAPORATION, temperature 292.0K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97918 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 14, 2011
RadiationMonochromator: Cryo-Cooled double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 3.73→50 Å / Num. all: 38382 / Num. obs: 38152 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 3.6 % / Rmerge(I) obs: 0.119 / Net I/σ(I): 7.4
Reflection shellResolution: 3.73→3.9 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.656 / Mean I/σ(I) obs: 2.1 / % possible all: 100

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Processing

Software
NameVersionClassification
JBluIce-EPICSdata collection
PHASESphasing
PHENIX(phenix.refine: 1.7.2_869)refinement
MOSFLMdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.73→49.202 Å / SU ML: 1.04 / σ(F): 1.09 / Phase error: 29.98 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2932 1906 5.02 %RANDOM
Rwork0.2678 ---
obs0.2691 38136 98.65 %-
all-38175 --
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 87.902 Å2 / ksol: 0.3 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-13.411 Å20 Å2-0 Å2
2--17.0212 Å2-0 Å2
3----30.4322 Å2
Refinement stepCycle: LAST / Resolution: 3.73→49.202 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15322 0 0 0 15322
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00915668
X-RAY DIFFRACTIONf_angle_d1.12721377
X-RAY DIFFRACTIONf_dihedral_angle_d14.275655
X-RAY DIFFRACTIONf_chiral_restr0.0832404
X-RAY DIFFRACTIONf_plane_restr0.0042767
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.73-3.77910.36041430.35282696X-RAY DIFFRACTION99
3.7791-3.83080.35711250.34642618X-RAY DIFFRACTION99
3.8308-3.88550.33581390.32792637X-RAY DIFFRACTION99
3.8855-3.94350.31011790.30892602X-RAY DIFFRACTION100
3.9435-4.00510.33671290.312673X-RAY DIFFRACTION99
4.0051-4.07070.31571410.30282625X-RAY DIFFRACTION100
4.0707-4.14090.33681500.29672613X-RAY DIFFRACTION99
4.1409-4.21610.24471180.29362660X-RAY DIFFRACTION99
4.2161-4.29720.32911250.27392697X-RAY DIFFRACTION99
4.2972-4.38480.33881280.27052601X-RAY DIFFRACTION99
4.3848-4.48010.26911400.2692661X-RAY DIFFRACTION99
4.4801-4.58430.29341640.24232567X-RAY DIFFRACTION99
4.5843-4.69880.22451460.2422629X-RAY DIFFRACTION99
4.6988-4.82580.29381500.23992639X-RAY DIFFRACTION99
4.8258-4.96760.2821520.25312583X-RAY DIFFRACTION99
4.9676-5.12780.28231070.23182679X-RAY DIFFRACTION99
5.1278-5.31090.31241210.2542621X-RAY DIFFRACTION98
5.3109-5.52330.37921420.27122611X-RAY DIFFRACTION98
5.5233-5.77430.30181170.26952600X-RAY DIFFRACTION98
5.7743-6.07820.35361490.28152621X-RAY DIFFRACTION98
6.0782-6.45820.30071280.28972608X-RAY DIFFRACTION98
6.4582-6.95560.38391470.2932591X-RAY DIFFRACTION98
6.9556-7.65320.30711390.27022566X-RAY DIFFRACTION97
7.6532-8.75530.29871500.2552574X-RAY DIFFRACTION97
8.7553-11.01030.20471260.2122621X-RAY DIFFRACTION98
11.0103-49.20620.25481440.26862555X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.06781.13862.77551.62711.3124.4398-0.22550.93051.0405-0.07950.90261.1343-1.78020.33870.231.99990.1432-0.37891.65540.08612.7738-66.202654.604336.5647
23.97451.78530.04697.45020.09482.9799-0.2782-0.05930.99910.17560.22890.6239-1.1882-0.28470.07091.523-0.0423-0.43820.96940.21221.6364-57.314450.068440.3327
31.2028-1.65791.00166.5526-2.49214.12050.35860.3398-0.4390.33750.6514-0.0149-0.1659-0.8323-0.38051.19540.0645-0.42141.3572-0.08461.933-58.697747.006638.6099
42.87991.27463.37836.1620.01683.5565-0.2396-0.03410.8243-0.71880.3082-0.0199-0.113-0.0852-0.15390.7767-0.1994-0.23840.8954-0.12231.4402-42.372137.046144.612
54.6976-3.0195-1.05637.36173.41026.30930.4154-0.397-0.4068-0.3836-0.34370.45970.53940.3569-0.33370.783-0.2616-0.36130.7048-0.0021.6755-44.207930.289547.8
610.46151.49-4.13836.09915.76813.14170.3747-0.75291.7534-1.0952-0.12931.3355-0.65441.7778-0.59420.8889-0.2352-0.04260.90850.01281.6117-38.870539.883853.4594
70.978-0.6378-1.8270.62641.78484.21730.3930.2748-0.95240.81511.09960.22660.7488-0.3-0.42511.7190.166-0.39861.17790.20.8541-46.6038-12.3637.0395
82.25762.78410.41775.5394-0.7686.4299-0.2946-0.40870.2325-0.17690.10960.70060.2545-0.0924-0.06851.07030.2494-0.29040.93130.11690.8678-51.4862-5.703736.5404
97.8003-0.20583.87874.83120.39394.7387-0.1132-0.1333-0.0486-0.30420.1339-0.6907-0.50370.45150.06910.70910.2137-0.20240.95650.14690.8531-33.9339-31.349852.154
106.1782-0.2153-3.55876.88631.20425.81231.08320.34650.7278-0.95440.0663-0.4636-0.48330.2196-0.81991.1320.3344-0.29261.17-0.01281.1479-37.4744-32.097744.1897
111.978-0.0568-1.190.89811.06912.1220.99320.2924-0.8647-1.4254-0.68780.91341.08240.3455-0.46821.1759-0.0512-0.40811.02980.1651.1971-42.1949-39.13645.724
123.8172-1.1812-7.26841.81346.85612.0791-3.2285.2745-2.3251-0.52572.57342.94761.6201-1.8025-0.8797-0.48452.0919-1.6783-2.70283.09990.4896-40.5322-42.868655.7052
139.05644.72342.81927.1297-0.56466.90410.3124-1.41681.04720.424-0.8212.12931.1133-3.4353-0.02370.7966-0.2152-0.09821.54030.2050.9659-46.3836-33.394352.9971
148.5581-1.3727-3.04311.95640.94071.8581-0.68220.67522.039-1.15670.4398-1.3345-0.8965-0.22530.15052.148-0.16180.01650.70910.0641.5853-41.017825.479414.18
151.7071.81450.73463.39620.27850.4663-0.38031.18562.7785-0.5112-1.24581.85191.6883-1.1583-0.01922.54940.2488-0.76830.40840.3781.9678-56.62128.718316.8263
168.3724-0.98471.58165.27461.46155.04010.10571.48640.4252-1.61011.03990.77340.1242-0.9824-0.75631.9391-0.1106-0.5031.17280.16451.3359-52.088120.526513.3674
172.8763-2.13970.50034.23950.46134.0088-0.3673-0.29560.264-0.75040.563-0.50530.0925-0.2726-0.12131.3472-0.215-0.21940.90010.02251.116-45.648517.131823.88
184.24541.83791.28131.33530.93091.1697-0.13040.9204-0.4653-1.22050.4874-0.34750.49370.5584-0.14132.0790.1984-0.18491.5705-0.22850.8712-37.3084-11.59886.5126
193.4491-0.75771.40034.10181.18572.8475-0.03091.3571-0.5285-1.31020.37830.4349-0.23430.6459-0.37921.8659-0.0573-0.1591.1842-0.2690.6785-42.3807-10.60048.594
205.1473-2.05475.77465.4384-0.35957.40611.1207-0.3047-2.4474-1.03271.3436-0.09710.0097-1.3096-0.71694.0991-0.1054-1.3481.5967-0.6153.3048-50.4812-46.9193-5.4313
215.56160.54791.66812.9881-1.89611.95010.85581.1777-1.2372-1.21550.76330.13541.92350.8875-0.64782.64720.252-0.79742.5926-0.64842.0298-49.6783-34.5973-3.4309
222.82893.23962.78773.70464.00343.83971.0245-2.8991-3.491.4125-0.06690.34021.0377-0.712-0.50832.76520.2795-0.19062.54090.00842.8065-50.3349-37.17787.1139
230.88360.15530.30930.53370.84740.60780.7668-1.3642-3.55750.5524-1.5209-0.83090.69990.4705-0.47862.28580.0844-0.23171.64380.462.4838-44.5185-39.79738.0991
240.8574-0.70041.89250.3216-0.63571.4648-2.40480.4429-3.15751.64290.10340.15361.38230.1612-0.90623.13380.8999-1.11712.0084-1.02492.9028-37.496-43.49953.0493
251.633-0.0443-0.18464.93820.81710.08710.05691.2496-2.94-1.07894.0189-2.67030.97263.0723-1.08432.7160.9291-1.08073.1273-0.66853.4559-35.4955-39.66330.1161
266.92875.99341.03472.05356.195.56250.6949-2.52320.05892.1544-1.45750.24112.0376-1.54220.98841.7728-0.12320.11331.76840.08491.4629-93.6345-55.995875.6989
274.51710.4879-2.35061.53070.99042.63910.5755-0.8504-0.1140.7705-0.3049-0.31240.4569-0.623-0.29790.9865-0.24350.04411.02660.17621.4604-84.2137-50.7868.2801
287.38241.52342.55016.49821.29934.64111.05980.2718-0.5125-0.1793-0.38420.41290.0934-0.1353-0.41490.79910.09890.07340.8604-0.11141.3271-67.39-46.390960.1114
291.5651.59510.46563.23961.8324.1926-0.70821.06590.8980.68390.44231.02331.5859-0.02490.43521.6371-0.12930.13221.2543-0.18360.4209-44.5353-5.314570.6225
302.54870.0182-1.11314.76081.07272.4233-1.1130.91340.6313-0.42960.08080.56820.39170.99140.58351.0612-0.1933-0.11711.798-0.01650.8664-50.1975-5.415563.6617
314.73393.9296-1.22785.8439-1.06296.0545-0.1473-0.26870.45780.12840.27680.9177-0.5116-0.2906-0.09220.80180.07570.15431.16510.10160.7487-56.0427-7.32374.389
324.21352.7418-1.65382.2231-1.45961.5902-0.36830.87110.1234-0.5377-0.1055-0.00940.25310.020.22580.9015-0.0733-0.04011.12950.06080.7275-34.8241-3.464660.3414
332.7314-1.6995-0.8232.8928-0.70494.6373-0.5354-0.57360.010.63270.3074-1.3111-0.10231.93830.28431.0287-0.3472-0.16872.07780.05891.2607-22.60972.347259.6122
345.15340.16651.78735.3968-0.38673.8566-0.14350.1495-0.54081.0846-0.2003-0.74320.28010.812-0.56351.346-0.4485-0.31912.03460.23221.4503-25.96666.189263.7724
350.03920.0759-0.0719-0.0791-0.05040.0527-0.1738-0.26651.1362-0.03710.69010.0336-0.5704-0.3037-0.03591.4341-0.34430.00991.80320.32771.446-24.643715.57358.5173
363.18450.3677-2.19273.5778-1.7622.32770.4581-0.22410.8438-0.5441-0.73022.6319-0.7874-1.46690.12651.05990.0487-0.57811.48040.24031.7175-32.602612.291654.9774
372.76641.07862.34491.87241.75092.12260.4484-0.6038-0.55311.08250.18450.07031.3639-0.8737-0.6981.5744-0.7069-0.00011.57990.32161.2516-59.383-40.757393.2073
38-0.0722-0.01520.23872.34420.32050.01350.9619-0.8666-0.7431.5516-0.33991.7012-1.2095-0.2762-0.37481.5968-0.54090.55692.146-0.07511.8763-74.4121-35.147491.3089
392.4997-1.52030.56611.0623-0.19973.27811.08-1.5790.17621.6796-2.01130.376-0.08450.35320.70361.7504-0.32030.6991.87920.19741.259-63.5852-30.052698.6647
402.07871.70541.39831.4454-0.77384.46890.7970.31150.38121.4066-0.0104-0.21370.4547-0.9341-0.52691.0796-0.33130.35691.57960.12231.0858-67.1127-32.937888.3168
414.9536-0.58992.39387.8501-2.98815.80790.5584-0.10210.0688-0.5749-0.42421.16320.1807-1.4201-0.3981.527-0.18580.23251.6714-0.03831.3627-66.1633-23.315785.0198
421.7673-0.2009-1.41573.33161.11283.00510.47860.9219-0.35630.59410.5083-0.2962-0.12910.3387-0.39631.0352-0.1458-0.08461.4794-0.13841.0774-55.3711-32.643383.4394
435.7507-0.52961.97924.31660.68764.33560.2207-0.89890.29231.2415-0.2334-0.23280.02740.12960.00631.3051-0.25530.05981.1273-0.10310.4937-41.0216-13.100497.7778
442.67030.3756-2.03994.02991.08071.9111-0.1596-0.74911.86354.05642.3225-0.87562.0883-0.2269-1.07182.98160.1801-0.56922.3265-0.73552.1827-28.985917.7087112.9976
455.6155-2.3495-1.39756.1422.48353.17310.34150.62153.6881.3608-0.7602-1.2634-1.8777-0.2595-1.19133.126-0.2251-0.15292.2278-0.20452.07-35.175416.2864105.2066
465.3417-2.52850.88586.3268-1.44391.32530.97061.15631.0733-2.72130.7545-1.1864-0.48930.4546-1.13642.5642-0.12230.56812.065-0.19682.5722-28.676716.865599.4191
471.69021.1853-0.90020.2695-0.21150.3458-0.7231.53840.6127-0.49160.4125-3.6415-1.4491-0.4202-1.14732.9209-0.54560.82982.7259-1.75693.5394-20.601416.4458104.2291
488.73522.6585-0.25347.1016-1.11.43040.9119-1.41852.5476-0.70012.7558-4.2381-3.07310.2121-1.33832.9878-0.24140.61712.6621-0.74563.8133-20.713112.0673107.0817
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resseq 380:391)
2X-RAY DIFFRACTION2chain 'A' and (resseq 392:474)
3X-RAY DIFFRACTION3chain 'A' and (resseq 475:507)
4X-RAY DIFFRACTION4chain 'A' and (resseq 508:551)
5X-RAY DIFFRACTION5chain 'A' and (resseq 552:612)
6X-RAY DIFFRACTION6chain 'A' and (resseq 613:626)
7X-RAY DIFFRACTION7chain 'B' and (resseq 9:26)
8X-RAY DIFFRACTION8chain 'B' and (resseq 27:150)
9X-RAY DIFFRACTION9chain 'B' and (resseq 151:222)
10X-RAY DIFFRACTION10chain 'B' and (resseq 223:249)
11X-RAY DIFFRACTION11chain 'B' and (resseq 250:267)
12X-RAY DIFFRACTION12chain 'B' and (resseq 268:279)
13X-RAY DIFFRACTION13chain 'B' and (resseq 280:294)
14X-RAY DIFFRACTION14chain 'C' and (resseq 8:50)
15X-RAY DIFFRACTION15chain 'C' and (resseq 51:60)
16X-RAY DIFFRACTION16chain 'C' and (resseq 61:93)
17X-RAY DIFFRACTION17chain 'C' and (resseq 94:146)
18X-RAY DIFFRACTION18chain 'D' and (resseq 9:70)
19X-RAY DIFFRACTION19chain 'D' and (resseq 71:169)
20X-RAY DIFFRACTION20chain 'D' and (resseq 170:181)
21X-RAY DIFFRACTION21chain 'D' and (resseq 182:195)
22X-RAY DIFFRACTION22chain 'D' and (resseq 196:216)
23X-RAY DIFFRACTION23chain 'D' and (resseq 217:248)
24X-RAY DIFFRACTION24chain 'D' and (resseq 249:274)
25X-RAY DIFFRACTION25chain 'D' and (resseq 275:294)
26X-RAY DIFFRACTION26chain 'E' and (resseq 380:405)
27X-RAY DIFFRACTION27chain 'E' and (resseq 406:524)
28X-RAY DIFFRACTION28chain 'E' and (resseq 525:626)
29X-RAY DIFFRACTION29chain 'F' and (resseq 9:26)
30X-RAY DIFFRACTION30chain 'F' and (resseq 27:53)
31X-RAY DIFFRACTION31chain 'F' and (resseq 54:121)
32X-RAY DIFFRACTION32chain 'F' and (resseq 122:181)
33X-RAY DIFFRACTION33chain 'F' and (resseq 182:222)
34X-RAY DIFFRACTION34chain 'F' and (resseq 223:249)
35X-RAY DIFFRACTION35chain 'F' and (resseq 250:279)
36X-RAY DIFFRACTION36chain 'F' and (resseq 280:294)
37X-RAY DIFFRACTION37chain 'G' and (resseq 8:50)
38X-RAY DIFFRACTION38chain 'G' and (resseq 51:60)
39X-RAY DIFFRACTION39chain 'G' and (resseq 61:75)
40X-RAY DIFFRACTION40chain 'G' and (resseq 76:101)
41X-RAY DIFFRACTION41chain 'G' and (resseq 102:113)
42X-RAY DIFFRACTION42chain 'G' and (resseq 114:146)
43X-RAY DIFFRACTION43chain 'H' and (resseq 9:150)
44X-RAY DIFFRACTION44chain 'H' and (resseq 151:181)
45X-RAY DIFFRACTION45chain 'H' and (resseq 182:216)
46X-RAY DIFFRACTION46chain 'H' and (resseq 217:248)
47X-RAY DIFFRACTION47chain 'H' and (resseq 249:274)
48X-RAY DIFFRACTION48chain 'H' and (resseq 275:294)

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