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Yorodumi- PDB-4e45: Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Com... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4.0E+45 | ||||||
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Title | Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM | ||||||
Components |
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Keywords | DNA BINDING PROTEIN/HYDROLASE / Histone-Fold / Pentamer / Fanconi Anemia / Helicase / FANCM / MHF / DNA BINDING PROTEIN-HYDROLASE complex | ||||||
Function / homology | Function and homology information double-strand break repair via synthesis-dependent strand annealing / FANCM-MHF complex / Fanconi anaemia nuclear complex / inner kinetochore / resolution of meiotic recombination intermediates / four-way junction helicase activity / nuclease activity / kinetochore assembly / 3'-5' DNA helicase activity / positive regulation of protein monoubiquitination ...double-strand break repair via synthesis-dependent strand annealing / FANCM-MHF complex / Fanconi anaemia nuclear complex / inner kinetochore / resolution of meiotic recombination intermediates / four-way junction helicase activity / nuclease activity / kinetochore assembly / 3'-5' DNA helicase activity / positive regulation of protein monoubiquitination / replication fork processing / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / interstrand cross-link repair / Mitotic Prometaphase / four-way junction DNA binding / EML4 and NUDC in mitotic spindle formation / Deposition of new CENPA-containing nucleosomes at the centromere / Resolution of Sister Chromatid Cohesion / positive regulation of protein ubiquitination / chromosome segregation / RHO GTPases Activate Formins / Fanconi Anemia Pathway / PKR-mediated signaling / Separation of Sister Chromatids / RNA helicase activity / RNA helicase / protein heterodimerization activity / cell division / DNA repair / DNA damage response / chromatin binding / chromatin / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2 Å | ||||||
Authors | Fox III, D. / Zhao, Y. / Yang, W. / Weidong, W. | ||||||
Citation | Journal: To be Published Title: Crystal Structures Reveal that FANCM remodels the MHF Tetramer in favor of binding Branched DNA Authors: Fox III, D. / Yan, Z. / Ling, C. / Zhao, Y. / Lee, D.Y. / Yang, W. / Weidong, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4e45.cif.gz | 286.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4e45.ent.gz | 226.8 KB | Display | PDB format |
PDBx/mmJSON format | 4e45.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e4/4e45 ftp://data.pdbj.org/pub/pdb/validation_reports/e4/4e45 | HTTPS FTP |
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-Related structure data
Related structure data | 4e44C 1tafS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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-Components
-Centromere protein ... , 2 types, 12 molecules ACFHKMBDGILN
#1: Protein | Mass: 13042.696 Da / Num. of mol.: 6 / Fragment: UNP residues 1-110 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: APITD1, CENPS, FAAP16, MHF1 / Plasmid: pGEX/pHis bicistronic / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8N2Z9 #2: Protein | Mass: 9116.545 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: STRA13, CENPX, FAAP10, MHF2 / Plasmid: pGEX/pHis bicistronic / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A8MT69 |
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-Protein , 1 types, 3 molecules EJO
#3: Protein | Mass: 16333.175 Da / Num. of mol.: 3 / Fragment: UNP residues 667-800 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FANCM, KIAA1596 / Plasmid: parallel MBP / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8IYD8, RNA helicase |
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-Non-polymers , 3 types, 551 molecules
#4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.31 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.8 Details: Tracking Code C4, 0.1M Tris pH 7.8, 0.2M LiCl, 0.1M Na2SO4, 17.5% w/v PEG3350, 10% glycerol cryo., vapor diffusion, sitting drop, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.03318 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 9, 2011 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.03318 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2→49.51 Å / Num. all: 120454 / Num. obs: 119106 / % possible obs: 98.9 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 3.5 % / Biso Wilson estimate: 40.705 Å2 / Rmerge(I) obs: 0.052 / Net I/σ(I): 14.9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1TAF Resolution: 2→49.51 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.936 / SU B: 4.298 / SU ML: 0.117 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.165 / ESU R Free: 0.156 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.212 Å2
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Refinement step | Cycle: LAST / Resolution: 2→49.51 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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