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Yorodumi- PDB-4bk5: crystal structure of the human EphA4 ectodomain in complex with h... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4bk5 | ||||||
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Title | crystal structure of the human EphA4 ectodomain in complex with human ephrin A5 (amine-methylated sample) | ||||||
Components |
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Keywords | SIGNALING PROTEIN / CELL ADHESION / CELL REPULSION / RECEPTOR CLUSTERING / RECEPTOR CIS INTERACTION / ERYTHROPOETIN-PRODUCING HEPATOCELLULAR RECEPTOR / LBD / EGF / FN | ||||||
Function / homology | Function and homology information neurotrophin TRKC receptor binding / neurotrophin TRKB receptor binding / DH domain binding / neuron projection fasciculation / corticospinal tract morphogenesis / regulation of astrocyte differentiation / negative regulation of proteolysis involved in protein catabolic process / positive regulation of Rho guanyl-nucleotide exchange factor activity / neuron projection guidance / nephric duct morphogenesis ...neurotrophin TRKC receptor binding / neurotrophin TRKB receptor binding / DH domain binding / neuron projection fasciculation / corticospinal tract morphogenesis / regulation of astrocyte differentiation / negative regulation of proteolysis involved in protein catabolic process / positive regulation of Rho guanyl-nucleotide exchange factor activity / neuron projection guidance / nephric duct morphogenesis / regulation of synapse pruning / fasciculation of sensory neuron axon / fasciculation of motor neuron axon / synapse pruning / negative regulation of substrate adhesion-dependent cell spreading / negative regulation of cellular response to hypoxia / negative regulation of axon regeneration / positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process / PH domain binding / glial cell migration / synaptic membrane adhesion / regulation of cell-cell adhesion / transmembrane-ephrin receptor activity / GPI-linked ephrin receptor activity / collateral sprouting / regulation of modification of synaptic structure / cellular response to follicle-stimulating hormone stimulus / positive regulation of collateral sprouting / negative regulation of epithelial to mesenchymal transition / regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of dendritic spine morphogenesis / neurotrophin TRKA receptor binding / chemorepellent activity / negative regulation of cell adhesion / adult walking behavior / motor neuron axon guidance / regulation of cell morphogenesis / retinal ganglion cell axon guidance / positive regulation of synapse assembly / adherens junction organization / positive regulation of dendrite morphogenesis / EPH-Ephrin signaling / Somitogenesis / regulation of focal adhesion assembly / regulation of axonogenesis / positive regulation of amyloid-beta formation / cochlea development / regulation of GTPase activity / EPHA-mediated growth cone collapse / transmembrane receptor protein tyrosine kinase activator activity / negative regulation of long-term synaptic potentiation / EPH-ephrin mediated repulsion of cells / basement membrane / positive regulation of cell adhesion / GABA-ergic synapse / ephrin receptor signaling pathway / positive regulation of protein tyrosine kinase activity / axon terminus / axonal growth cone / regulation of microtubule cytoskeleton organization / cellular response to forskolin / protein tyrosine kinase binding / ephrin receptor binding / negative regulation of cell migration / dendritic shaft / filopodium / caveola / axon guidance / regulation of actin cytoskeleton organization / postsynaptic density membrane / adherens junction / neuromuscular junction / Schaffer collateral - CA1 synapse / negative regulation of ERK1 and ERK2 cascade / receptor protein-tyrosine kinase / peptidyl-tyrosine phosphorylation / cellular response to amyloid-beta / positive regulation of peptidyl-tyrosine phosphorylation / negative regulation of neuron projection development / presynaptic membrane / nervous system development / amyloid-beta binding / kinase activity / perikaryon / early endosome membrane / protein tyrosine kinase activity / mitochondrial outer membrane / negative regulation of translation / dendritic spine / protein autophosphorylation / protein stabilization / cell adhesion / protein kinase activity / positive regulation of cell migration / positive regulation of protein phosphorylation / axon / external side of plasma membrane / dendrite / glutamatergic synapse / positive regulation of cell population proliferation Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4 Å | ||||||
Authors | Seiradake, E. / Schaupp, A. / del Toro Ruiz, D. / Kaufmann, R. / Mitakidis, N. / Harlos, K. / Aricescu, A.R. / Klein, R. / Jones, E.Y. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2013 Title: Structurally Encoded Intraclass Differences in Epha Clusters Drive Distinct Cell Responses Authors: Seiradake, E. / Schaupp, A. / Del Toro Ruiz, D. / Kaufmann, R. / Mitakidis, N. / Harlos, K. / Aricescu, A.R. / Klein, R. / Jones, E.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bk5.cif.gz | 275.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bk5.ent.gz | 225.9 KB | Display | PDB format |
PDBx/mmJSON format | 4bk5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bk/4bk5 ftp://data.pdbj.org/pub/pdb/validation_reports/bk/4bk5 | HTTPS FTP |
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-Related structure data
Related structure data | 4bk4SC 4bkaC 4bkfC 2x11S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 62649.336 Da / Num. of mol.: 1 / Fragment: HEPHA4 ECTODOMAIN, RESIDUES 20-547 Source method: isolated from a genetically manipulated source Details: SAMPLE WAS AMINE-METHYLATED PRIOR TO CRYSTALLIZATION Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PHLSEC / Cell line (production host): HEK293T / Production host: HOMO SAPIENS (human) References: UniProt: P54764, receptor protein-tyrosine kinase |
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#2: Protein | Mass: 20689.596 Da / Num. of mol.: 1 Fragment: HEPHRINA5 RECEPTOR BINDING DOMAIN, RESIDUES 27-166 Source method: isolated from a genetically manipulated source Details: SAMPLE WAS AMINE-METHYLATED PRIOR TO CRYSTALLIZATION Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PHLSEC / Cell line (production host): HEK293T / Production host: HOMO SAPIENS (human) References: UniProt: P52803, receptor protein-tyrosine kinase |
Sequence details | SEQUENCE CORRESPONDS TO HEPHA4 RESIDUES 27-547 FUSED TO AN N-TERMINAL SECRETION SIGNAL SEQUENCE AND ...SEQUENCE CORRESPOND |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density % sol: 88.2 % Description: WE USED THE NEW CC CRITERIA TO DETERMINE THE HIGH RESOLUTION CUT-OFF. THE CC-HALF VALUE IN THE HIGHEST RES SHELL IS 15.9 |
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Crystal grow | Details: 8 % W-V POLYETHYLENE GLYCOL 8000 AND 0.1 M TRIS-HCL PH 8.5 |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||
Reflection | Resolution: 4→70 Å / Num. obs: 27221 / % possible obs: 99.9 % / Observed criterion σ(I): 0 / Redundancy: 11.3 % / Biso Wilson estimate: 130.48 Å2 / Rmerge(I) obs: 0.41 / Net I/σ(I): 7.7 | ||||||||||||||||||||
Reflection shell | Resolution: 4→4.05 Å / Redundancy: 11.3 % / Mean I/σ(I) obs: 0.59 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRIES 4BK4, 2X11 Resolution: 4→70.41 Å / Cor.coef. Fo:Fc: 0.8006 / Cor.coef. Fo:Fc free: 0.8147 / SU R Cruickshank DPI: 1.078 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 1 / SU Rfree Blow DPI: 0.537 / SU Rfree Cruickshank DPI: 0.555
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Displacement parameters | Biso mean: 187.97 Å2
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Refine analyze | Luzzati coordinate error obs: 1.707 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4→70.41 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 4→4.15 Å / Total num. of bins used: 14
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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