+Open data
-Basic information
Entry | Database: PDB / ID: 3tv0 | ||||||
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Title | Structure of dynactin p27 subunit | ||||||
Components | Dynactin subunit 6 | ||||||
Keywords | STRUCTURAL PROTEIN / left-handed beta-helix / p25 / p62 / Arp11 / cytosol | ||||||
Function / homology | Function and homology information kinetochore => GO:0000776 / dynactin complex / COPI-independent Golgi-to-ER retrograde traffic / endoplasmic reticulum to Golgi vesicle-mediated transport / COPI-mediated anterograde transport / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / antigen processing and presentation of exogenous peptide antigen via MHC class II / centrosome / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1492 Å | ||||||
Authors | Derewenda, Z.S. / Derewenda, U. / Kowalska, A. | ||||||
Citation | Journal: To be Published Title: Dynactin targets Polo-like kinase 1 to kinetochores Authors: Derewenda, Z.S. / Derewenda, U. / Kowalska, A. / Zheng, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3tv0.cif.gz | 63.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3tv0.ent.gz | 50.3 KB | Display | PDB format |
PDBx/mmJSON format | 3tv0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tv/3tv0 ftp://data.pdbj.org/pub/pdb/validation_reports/tv/3tv0 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 21087.285 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: DCTN6, WS3 / Production host: Escherichia coli (E. coli) / References: UniProt: O00399 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.79 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 3.6 Details: 19% PEG 2000, 0.15 M Potassium phosphate, pH 3.6, VAPOR DIFFUSION, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 27, 2010 / Details: Si(111) |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.149→43.5 Å / Num. all: 16307 / Num. obs: 16255 / % possible obs: 81.1 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 5 |
Reflection shell | Resolution: 2.149→2.19 Å / % possible all: 38.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1492→43.451 Å / SU ML: 0.33 / σ(F): 1.36 / Phase error: 30.36 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 46.955 Å2 / ksol: 0.385 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.1492→43.451 Å
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Refine LS restraints |
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LS refinement shell |
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