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Yorodumi- PDB-2zxr: Crystal structure of RecJ in complex with Mg2+ from Thermus therm... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2zxr | ||||||
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Title | Crystal structure of RecJ in complex with Mg2+ from Thermus thermophilus HB8 | ||||||
Components | Single-stranded DNA specific exonuclease RecJ | ||||||
Keywords | HYDROLASE / nuclease / single-stranded DNA / DNA repair / Exonuclease | ||||||
Function / homology | Function and homology information exonuclease activity / Hydrolases; Acting on ester bonds / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.15 Å | ||||||
Authors | Wakamatsu, T. / Kitamura, Y. / Nakagawa, N. / Masui, R. / Kuramitsu, S. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2010 Title: Structure of RecJ exonuclease defines its specificity for single-stranded DNA Authors: Wakamatsu, T. / Kitamura, Y. / Kotera, Y. / Nakagawa, N. / Kuramitsu, S. / Masui, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2zxr.cif.gz | 137.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2zxr.ent.gz | 106.3 KB | Display | PDB format |
PDBx/mmJSON format | 2zxr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/2zxr ftp://data.pdbj.org/pub/pdb/validation_reports/zx/2zxr | HTTPS FTP |
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-Related structure data
Related structure data | 2zxoSC 2zxpC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 72960.344 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Gene: recj, TTHA1167 / Plasmid: pET11a / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta2(DE3)pLysS References: UniProt: Q5SJ47, Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters |
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#2: Chemical | ChemComp-MG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.84 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.05M ADA, 6% PEG4000, 1% 2-propanol. The crystal was soaked in cryoprotecting solution containg 20% glycerol and 10mM magnesium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B2 / Wavelength: 1 Å |
Detector | Type: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Jul 18, 2008 |
Radiation | Monochromator: Si double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→50 Å / Num. all: 48684 / Num. obs: 48684 / % possible obs: 98.5 % / Observed criterion σ(I): -3 / Redundancy: 17.2 % / Biso Wilson estimate: 20.1 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 47.2 |
Reflection shell | Resolution: 2.15→2.23 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.276 / Mean I/σ(I) obs: 3.3 / Num. unique all: 837996 / % possible all: 86 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2ZXO Resolution: 2.15→43.01 Å / Isotropic thermal model: restrained / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 34.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.15→43.01 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.28 Å / Rfactor Rfree error: 0.012
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