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Yorodumi- PDB-2p55: X-ray structure of the human mitogen-activated protein kinase kin... -
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-Basic information
Entry | Database: PDB / ID: 2p55 | ||||||
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Title | X-ray structure of the human mitogen-activated protein kinase kinase 1 (MEK1) in a complex with ligand and MgATP | ||||||
Components | Dual specificity mitogen-activated protein kinase kinase 1 | ||||||
Keywords | TRANSFERASE / protein kinase structure / Mitogen activated protein kinase kinase / signal transduction / ligand co-complex / ternary co-complex with kinase / ligand and MgATP / non-competitive protein kinase inhibitor | ||||||
Function / homology | Function and homology information epithelial cell proliferation involved in lung morphogenesis / positive regulation of endodermal cell differentiation / placenta blood vessel development / regulation of axon regeneration / mitogen-activated protein kinase kinase / type B pancreatic cell proliferation / labyrinthine layer development / MAP-kinase scaffold activity / cerebellar cortex formation / Signaling by MAP2K mutants ...epithelial cell proliferation involved in lung morphogenesis / positive regulation of endodermal cell differentiation / placenta blood vessel development / regulation of axon regeneration / mitogen-activated protein kinase kinase / type B pancreatic cell proliferation / labyrinthine layer development / MAP-kinase scaffold activity / cerebellar cortex formation / Signaling by MAP2K mutants / regulation of Golgi inheritance / trachea formation / regulation of early endosome to late endosome transport / Negative feedback regulation of MAPK pathway / regulation of stress-activated MAPK cascade / positive regulation of axonogenesis / Frs2-mediated activation / protein kinase activator activity / ERBB2-ERBB3 signaling pathway / face development / endodermal cell differentiation / MAPK3 (ERK1) activation / Bergmann glial cell differentiation / MAP kinase kinase activity / thyroid gland development / Uptake and function of anthrax toxins / Schwann cell development / keratinocyte differentiation / ERK1 and ERK2 cascade / myelination / protein serine/threonine/tyrosine kinase activity / protein serine/threonine kinase activator activity / MAP3K8 (TPL2)-dependent MAPK1/3 activation / insulin-like growth factor receptor signaling pathway / thymus development / Signal transduction by L1 / cell motility / RAF activation / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / positive regulation of protein serine/threonine kinase activity / neuron differentiation / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / chemotaxis / MAPK cascade / cellular senescence / Signaling by BRAF and RAF1 fusions / late endosome / heart development / scaffold protein binding / protein tyrosine kinase activity / positive regulation of ERK1 and ERK2 cascade / early endosome / protein kinase activity / negative regulation of cell population proliferation / protein phosphorylation / focal adhesion / protein serine kinase activity / centrosome / protein serine/threonine kinase activity / positive regulation of gene expression / positive regulation of DNA-templated transcription / Golgi apparatus / endoplasmic reticulum / signal transduction / mitochondrion / ATP binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.8 Å | ||||||
Authors | Ohren, J.F. / Pavlovsky, A.G. | ||||||
Citation | Journal: J.Med.Chem. / Year: 2007 Title: 4-anilino-5-carboxamido-2-pyridone derivatives as noncompetitive inhibitors of mitogen-activated protein kinase kinase. Authors: Spicer, J.A. / Rewcastle, G.W. / Kaufman, M.D. / Black, S.L. / Plummer, M.S. / Denny, W.A. / Quin, J. / Shahripour, A.B. / Barrett, S.D. / Whitehead, C.E. / Milbank, J.B. / Ohren, J.F. / ...Authors: Spicer, J.A. / Rewcastle, G.W. / Kaufman, M.D. / Black, S.L. / Plummer, M.S. / Denny, W.A. / Quin, J. / Shahripour, A.B. / Barrett, S.D. / Whitehead, C.E. / Milbank, J.B. / Ohren, J.F. / Gowan, R.C. / Omer, C. / Camp, H.S. / Esmaeil, N. / Moore, K. / Sebolt-Leopold, J.S. / Pryzbranowski, S. / Merriman, R.L. / Ortwine, D.F. / Warmus, J.S. / Flamme, C.M. / Pavlovsky, A.G. / Tecle, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2p55.cif.gz | 74.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2p55.ent.gz | 53.9 KB | Display | PDB format |
PDBx/mmJSON format | 2p55.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p5/2p55 ftp://data.pdbj.org/pub/pdb/validation_reports/p5/2p55 | HTTPS FTP |
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-Related structure data
Related structure data | 1s9jS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | May form functional homodimers. |
-Components
#1: Protein | Mass: 36859.469 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MAP2K1, MEK1, PRKMK1 / Production host: Escherichia coli (E. coli) References: UniProt: Q02750, mitogen-activated protein kinase kinase |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-MRA / |
#4: Chemical | ChemComp-ATP / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.46 % |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Nov 27, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 11439 / % possible obs: 99 % / Redundancy: 5 % / Rsym value: 0.083 |
Reflection shell | Resolution: 2.8→2.9 Å / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: 1S9J Resolution: 2.8→30 Å / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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