+Open data
-Basic information
Entry | Database: PDB / ID: 2mcz | ||||||
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Title | CR1 Sushi domains 1 and 2 | ||||||
Components | Complement receptor type 1 | ||||||
Keywords | IMMUNE SYSTEM / CR1 / PfRh4 / malaria / CCP | ||||||
Function / homology | Function and homology information complement component C4b receptor activity / immune complex clearance by erythrocytes / complement component C3b receptor activity / positive regulation of serine-type endopeptidase activity / negative regulation of complement activation, alternative pathway / complement component C4b binding / negative regulation of immunoglobulin production / negative regulation of activation of membrane attack complex / negative regulation of complement activation, classical pathway / negative regulation of complement-dependent cytotoxicity ...complement component C4b receptor activity / immune complex clearance by erythrocytes / complement component C3b receptor activity / positive regulation of serine-type endopeptidase activity / negative regulation of complement activation, alternative pathway / complement component C4b binding / negative regulation of immunoglobulin production / negative regulation of activation of membrane attack complex / negative regulation of complement activation, classical pathway / negative regulation of complement-dependent cytotoxicity / negative regulation of complement activation / ATP export / T cell mediated immunity / positive regulation of activation of membrane attack complex / plasma membrane organization / complement component C3b binding / negative regulation of plasma cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / negative regulation of serine-type endopeptidase activity / complement receptor mediated signaling pathway / positive regulation of regulatory T cell differentiation / complement activation, alternative pathway / negative regulation of interleukin-2 production / plasma membrane raft / ficolin-1-rich granule membrane / negative regulation of type II interferon production / complement activation, classical pathway / negative regulation of T cell proliferation / secretory granule membrane / Regulation of Complement cascade / virus receptor activity / cytoskeleton / positive regulation of cell population proliferation / Neutrophil degranulation / cell surface / extracellular space / extracellular exosome / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Park, H.J. / Guariento, M.J. / Maciejewski, M. / Hauart, R. / Tham, W. / Cowman, A.F. / Schmidt, C.Q. / Martens, H. / Liszewski, K.M. / Hourcade, D. ...Park, H.J. / Guariento, M.J. / Maciejewski, M. / Hauart, R. / Tham, W. / Cowman, A.F. / Schmidt, C.Q. / Martens, H. / Liszewski, K.M. / Hourcade, D. / Barlow, P.N. / Atkinson, J.P. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: Using Mutagenesis and Structural Biology to Map the Binding Site for the Plasmodium falciparum Merozoite Protein PfRh4 on the Human Immune Adherence Receptor. Authors: Park, H.J. / Guariento, M. / Maciejewski, M. / Hauhart, R. / Tham, W.H. / Cowman, A.F. / Schmidt, C.Q. / Mertens, H.D. / Liszewski, M.K. / Hourcade, D.E. / Barlow, P.N. / Atkinson, J.P. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mcz.cif.gz | 735.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mcz.ent.gz | 617.7 KB | Display | PDB format |
PDBx/mmJSON format | 2mcz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mc/2mcz ftp://data.pdbj.org/pub/pdb/validation_reports/mc/2mcz | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14300.222 Da / Num. of mol.: 1 / Fragment: SUSHI DOMAINS 1 AND 2(UNP RESIDUES 41-163) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CR1, C3BR / Plasmid: pPICZaB / Production host: Komagataella pastoris (fungus) / References: UniProt: P17927 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: Recombinant CR1 fragment, domains 1-2 | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 4.0 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |