+Open data
-Basic information
Entry | Database: PDB / ID: 2m37 | ||||||
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Title | Structure of lasso peptide astexin-1 | ||||||
Components | ASTEXIN-1 | ||||||
Keywords | UNKNOWN FUNCTION / ASTEXIN-1 / LASSO PEPTIDE / LARIAT PROTOKNOT | ||||||
Function / homology | defense response to bacterium / Astexin-1 Function and homology information | ||||||
Biological species | Asticcacaulis excentricus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Zimmermann, M. / Hegemann, J.D. / Xie, X. / Marahiel, M.A. | ||||||
Citation | Journal: Chem.Biol. / Year: 2013 Title: The astexin-1 lasso peptides: biosynthesis, stability, and structural studies. Authors: Zimmermann, M. / Hegemann, J.D. / Xie, X. / Marahiel, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m37.cif.gz | 116.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m37.ent.gz | 92.9 KB | Display | PDB format |
PDBx/mmJSON format | 2m37.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m3/2m37 ftp://data.pdbj.org/pub/pdb/validation_reports/m3/2m37 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2113.219 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Asticcacaulis excentricus (bacteria) / Strain: ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48 / Gene: ATXA / Strain (production host): CB48 / References: UniProt: E8RMD3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 5.7 mM ASTEXIN-1, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 5.7 mM / Component: ASTEXIN-1(19)-1 |
Sample conditions | Pressure: ambient atm / Temperature: 283 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |