Mass: 13077.996 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) artificial gene (others) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMgK
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR / Details: NMR structure refined with RDC data
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C arom NOESY
1
7
1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
8
1
3DC(CO)NH
1
9
1
3D (H)CCH-TOCSY
1
10
2
2D 1H-13C HSQC
1
11
3
2D 1H-15N HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1.073 mM [U-100% 13C; U-100% 15N] OR16.004, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.867 mM [U-100% 13C; U-100% 15N] OR16.006, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.859 mM [U-100% 13C; U-100% 15N] OR16.013, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O