[English] 日本語
Yorodumi- PDB-2l26: Rv0899 from Mycobacterium tuberculosis contains two separated domains -
+Open data
-Basic information
Entry | Database: PDB / ID: 2l26 | ||||||
---|---|---|---|---|---|---|---|
Title | Rv0899 from Mycobacterium tuberculosis contains two separated domains | ||||||
Components | Uncharacterized protein Rv0899/MT0922 | ||||||
Keywords | MEMBRANE PROTEIN / Rv0899 / OUT MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information response to host pH environment / ammonium transmembrane transport / peptidoglycan binding / cell wall / response to acidic pH / porin activity / plasma membrane => GO:0005886 / monoatomic ion transport / peptidoglycan-based cell wall / cell outer membrane ...response to host pH environment / ammonium transmembrane transport / peptidoglycan binding / cell wall / response to acidic pH / porin activity / plasma membrane => GO:0005886 / monoatomic ion transport / peptidoglycan-based cell wall / cell outer membrane / extracellular region / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Shi, C. / Li, J. / Gao, Y. / Wu, K. / Huang, H. / Tian, C. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2011 Title: Structural Studies of Mycobacterium tuberculosis Rv0899 Reveal a Monomeric Membrane-Anchoring Protein with Two Separate Domains Authors: Li, J. / Shi, C. / Gao, Y. / Wu, K. / Shi, P. / Lai, C. / Chen, L. / Wu, F. / Tian, C. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2l26.cif.gz | 724 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2l26.ent.gz | 622.5 KB | Display | PDB format |
PDBx/mmJSON format | 2l26.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/2l26 ftp://data.pdbj.org/pub/pdb/validation_reports/l2/2l26 | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 29443.129 Da / Num. of mol.: 1 / Fragment: residues 75-326 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Strain: H37RV / Production host: Escherichia coli (E. coli) / References: UniProt: P65593, UniProt: P9WIU5*PLUS |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1.0mM [U-100% 13C; U-100% 15N] Rv0899; 50mM sodium phosphate; 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | Ionic strength: 0.15 / pH: 7.2 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 / Representative conformer: 1 |