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Yorodumi- PDB-2kvu: Solution NMR Structure of SAP domain of MKL/myocardin-like protei... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kvu | ||||||
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Title | Solution NMR Structure of SAP domain of MKL/myocardin-like protein 1 from H.sapiens, Northeast Structural Genomics Consortium Target Target HR4547E | ||||||
Components | MKL/myocardin-like protein 1 | ||||||
Keywords | Transcription regulator / SAP motif / DNA/RNA binding / Structural Genomics / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-2 / Protein Structure Initiative / Chromosomal rearrangement / Coiled coil / Nucleus / Phosphoprotein / Polymorphism / Proto-oncogene / Transcription / Transcription regulation | ||||||
Function / homology | Function and homology information leucine zipper domain binding / smooth muscle cell differentiation / wound healing, spreading of cells / actin monomer binding / SUMOylation of transcription cofactors / RHO GTPases Activate Formins / positive regulation of miRNA transcription / actin binding / actin cytoskeleton organization / transcription coactivator activity ...leucine zipper domain binding / smooth muscle cell differentiation / wound healing, spreading of cells / actin monomer binding / SUMOylation of transcription cofactors / RHO GTPases Activate Formins / positive regulation of miRNA transcription / actin binding / actin cytoskeleton organization / transcription coactivator activity / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics, distance geometry, simulated annealing, torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Liu, G. / Xiao, R. / Janjua, J. / Acton, T.B. / Mao, B. / Everett, J. / Montelione, G.T. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: To be Published Title: Northeast Structural Genomics Consortium Target HR4547E Authors: Liu, G. / Xiao, R. / Janjua, J. / Acton, T.B. / Mao, B. / Everett, J. / Montelione, G.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kvu.cif.gz | 509.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kvu.ent.gz | 447.9 KB | Display | PDB format |
PDBx/mmJSON format | 2kvu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kv/2kvu ftp://data.pdbj.org/pub/pdb/validation_reports/kv/2kvu | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8161.401 Da / Num. of mol.: 1 / Fragment: SAP domain residues 336-396 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KIAA1438, MAL, MKL1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)+ Magic / References: UniProt: Q969V6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics, distance geometry, simulated annealing, torsion angle dynamics Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |