+Open data
-Basic information
Entry | Database: PDB / ID: 2kje | ||||||
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Title | NMR structure of CBP TAZ2 and adenoviral E1A complex | ||||||
Components |
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Keywords | TRANSCRIPTION / CBP / TAZ2 / E1A / adenoviral / Acetylation / Activator / Bromodomain / Chromosomal rearrangement / Disease mutation / Host-virus interaction / Isopeptide bond / Metal-binding / Methylation / Nucleus / Phosphoprotein / Transcription regulation / Transferase / Ubl conjugation / Zinc / Zinc-finger / Alternative splicing / DNA-binding / Early protein / Oncogene | ||||||
Function / homology | Function and homology information regulation by virus of viral protein levels in host cell / molecular sequestering activity / positive regulation of protein sumoylation / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / histone H3K27 acetyltransferase activity / regulation of smoothened signaling pathway / histone H3K18 acetyltransferase activity ...regulation by virus of viral protein levels in host cell / molecular sequestering activity / positive regulation of protein sumoylation / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / histone H3K27 acetyltransferase activity / regulation of smoothened signaling pathway / histone H3K18 acetyltransferase activity / N-terminal peptidyl-lysine acetylation / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / MRF binding / NFE2L2 regulates pentose phosphate pathway genes / NFE2L2 regulating MDR associated enzymes / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / negative regulation of transcription by RNA polymerase I / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of FOXO transcriptional activity by acetylation / Regulation of gene expression by Hypoxia-inducible Factor / Nuclear events mediated by NFE2L2 / Regulation of NFE2L2 gene expression / NOTCH3 Intracellular Domain Regulates Transcription / TRAF6 mediated IRF7 activation / peptide-lysine-N-acetyltransferase activity / NFE2L2 regulating anti-oxidant/detoxification enzymes / FOXO-mediated transcription of cell death genes / NFE2L2 regulating tumorigenic genes / embryonic digit morphogenesis / homeostatic process / Notch-HLH transcription pathway / protein acetylation / Formation of paraxial mesoderm / positive regulation of transforming growth factor beta receptor signaling pathway / non-canonical NF-kappaB signal transduction / Zygotic genome activation (ZGA) / acetyltransferase activity / stimulatory C-type lectin receptor signaling pathway / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / cellular response to nutrient levels / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / histone acetyltransferase complex / Attenuation phase / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / regulation of cellular response to heat / histone acetyltransferase activity / histone acetyltransferase / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RORA activates gene expression / NPAS4 regulates expression of target genes / Regulation of lipid metabolism by PPARalpha / CD209 (DC-SIGN) signaling / BMAL1:CLOCK,NPAS2 activates circadian gene expression / SUMOylation of transcription cofactors / Activation of gene expression by SREBF (SREBP) / Formation of the beta-catenin:TCF transactivating complex / protein destabilization / Heme signaling / Transcriptional activation of mitochondrial biogenesis / transcription coactivator binding / PPARA activates gene expression / Cytoprotection by HMOX1 / chromatin DNA binding / NOTCH1 Intracellular Domain Regulates Transcription / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / Pre-NOTCH Transcription and Translation / Transcriptional regulation of white adipocyte differentiation / Activation of anterior HOX genes in hindbrain development during early embryogenesis / positive regulation of protein localization to nucleus / transcription corepressor activity / cellular response to UV / rhythmic process / regulation of protein localization / Circadian Clock / p53 binding / TRAF3-dependent IRF activation pathway / HATs acetylate histones / symbiont-mediated perturbation of host ubiquitin-like protein modification / protein-containing complex assembly / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / damaged DNA binding / transcription coactivator activity / response to hypoxia / molecular adaptor activity / nuclear body / symbiont-mediated suppression of host gene expression / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / chromatin binding / positive regulation of cell population proliferation / chromatin / regulation of DNA-templated transcription / SARS-CoV-2 activates/modulates innate and adaptive immune responses / positive regulation of DNA-templated transcription Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Human adenovirus 5 | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Ferreon, J.C. / Martinez-Yamout, M. / Dyson, H. / Wright, P.E. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2009 Title: Structural basis for subversion of cellular control mechanisms by the adenoviral E1A oncoprotein. Authors: Ferreon, J.C. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kje.cif.gz | 808.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kje.ent.gz | 702.9 KB | Display | PDB format |
PDBx/mmJSON format | 2kje.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kj/2kje ftp://data.pdbj.org/pub/pdb/validation_reports/kj/2kje | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10527.567 Da / Num. of mol.: 1 / Fragment: UNP residues 1763-1854 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CREBBP, CBP / Plasmid: pET21a / Production host: Escherichia coli (E. coli) / References: UniProt: Q92793, histone acetyltransferase |
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#2: Protein/peptide | Mass: 4464.908 Da / Num. of mol.: 1 / Fragment: UNP residues 53-91 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human adenovirus 5 / Plasmid: pET21a / Production host: Escherichia coli (E. coli) / References: UniProt: P03255 |
#3: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1-1.5 mM [U-100% 13C; U-100% 15N] TRIS-1, 1-1.5 mM [U-100% 15N] TRIS-2, 100% D2O Solvent system: 100% D2O | |||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 6.8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |