+Open data
-Basic information
Entry | Database: PDB / ID: 2h9a | ||||||
---|---|---|---|---|---|---|---|
Title | Corrinoid Iron-Sulfur Protein | ||||||
Components |
| ||||||
Keywords | OXIDOREDUCTASE / heterodimer / beta-alpha-barrels | ||||||
Function / homology | Function and homology information acetyl-CoA catabolic process / methyltransferase activity / 4 iron, 4 sulfur cluster binding / iron ion binding Similarity search - Function | ||||||
Biological species | Carboxydothermus hydrogenoformans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 1.9 Å | ||||||
Authors | Dobbek, H. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2006 Title: Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis. Authors: Svetlitchnaia, T. / Svetlitchnyi, V. / Meyer, O. / Dobbek, H. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2h9a.cif.gz | 162.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2h9a.ent.gz | 125.6 KB | Display | PDB format |
PDBx/mmJSON format | 2h9a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h9/2h9a ftp://data.pdbj.org/pub/pdb/validation_reports/h9/2h9a | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 48469.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Carboxydothermus hydrogenoformans (bacteria) References: UniProt: Q3ACS3 |
---|---|
#2: Protein | Mass: 33910.355 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Carboxydothermus hydrogenoformans (bacteria) References: UniProt: Q3ACS0 |
-Non-polymers , 4 types, 583 molecules
#3: Chemical | ChemComp-IOD / #4: Chemical | ChemComp-B12 / | #5: Chemical | ChemComp-SF4 / | #6: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 1.87 Å3/Da / Density % sol: 34.39 % |
---|---|
Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 3350, 1mM beta-mercaptoethanol, 100 mM KI, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR571 / Wavelength: 1.5418 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jul 11, 2004 |
Radiation | Monochromator: Osmic mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→20 Å / Num. all: 48806 / Num. obs: 48659 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.9→2 Å / % possible all: 99.8 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MIR / Resolution: 1.9→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
| ||||||||||||||||||||
Refine LS restraints |
|