+Open data
-Basic information
Entry | Database: PDB / ID: 2fzf | ||||||
---|---|---|---|---|---|---|---|
Title | Hypothetical Protein Pfu-1136390-001 From Pyrococcus furiosus | ||||||
Components | hypothetical protein | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / SOUTHEAST COLLABORATORY FOR STRUCTURAL GENOMICS / SECSG / PROTEIN STRUCTURE INITIATIVE / PSI / CONSERVED HYPOTHETICAL PROTEIN / PYROCOCCUS FURIOSUS / HYPERTHERMOPHILE / Pfu-1136390-001 | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pyrococcus furiosus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.7 Å | ||||||
Authors | Fu, Z.-Q. / Liu, Z.-J. / Lee, D. / Kelley, L. / Chen, L. / Tempel, W. / Shah, N. / Horanyi, P. / Lee, H.S. / Habel, J. ...Fu, Z.-Q. / Liu, Z.-J. / Lee, D. / Kelley, L. / Chen, L. / Tempel, W. / Shah, N. / Horanyi, P. / Lee, H.S. / Habel, J. / Dillard, B.D. / Nguyen, D. / Chang, S.-H. / Zhang, H. / Chang, J. / Sugar, F.J. / Poole, F.L. / Jenney Jr., F.E. / Adams, M.W.W. / Rose, J.P. / Wang, B.-C. / Southeast Collaboratory for Structural Genomics (SECSG) | ||||||
Citation | Journal: To be published Title: Hypothetical Protein Pfu-1136390-001 From Pyrococcus furiosus Authors: Fu, Z.-Q. / Liu, Z.-J. / Lee, D. / Kelley, L. / Chen, L. / Tempel, W. / Shah, N. / Horanyi, P. / Lee, H.S. / Habel, J. / Dillard, B.D. / Nguyen, D. / Chang, S.-H. / Zhang, H. / Chang, J. / ...Authors: Fu, Z.-Q. / Liu, Z.-J. / Lee, D. / Kelley, L. / Chen, L. / Tempel, W. / Shah, N. / Horanyi, P. / Lee, H.S. / Habel, J. / Dillard, B.D. / Nguyen, D. / Chang, S.-H. / Zhang, H. / Chang, J. / Sugar, F.J. / Poole, F.L. / Jenney Jr., F.E. / Adams, M.W.W. / Rose, J.P. / Wang, B.-C. | ||||||
History |
| ||||||
Remark 300 | BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S) ...BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S). THE BIOLOGICAL UNIT OF THIS PROTEIN IS UNKNOWN. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2fzf.cif.gz | 76.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2fzf.ent.gz | 58.7 KB | Display | PDB format |
PDBx/mmJSON format | 2fzf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fz/2fzf ftp://data.pdbj.org/pub/pdb/validation_reports/fz/2fzf | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20739.184 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus furiosus (archaea) / Gene: PF1190 / Plasmid: PET24D BAM / Production host: Escherichia coli (E. coli) / Strain (production host): C41 PRIL / References: GenBank: 18893275, UniProt: Q8U1L6*PLUS #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.46 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 1600mM K2HPO4, 400mM NaH2PO4, 200mM NaCl, pH 8.0, temperature 291K, VAPOR DIFFUSION, SITTING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1.07 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Dec 11, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→20 Å / Num. all: 10830 / Num. obs: 10270 / % possible obs: 92 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.34 % / Biso Wilson estimate: 47.5 Å2 / Rmerge(I) obs: 0.062 / Rsym value: 0.062 / Net I/σ(I): 19.3 |
Reflection shell | Resolution: 2.7→2.81 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.183 / Mean I/σ(I) obs: 3.1 / Num. unique all: 852 / Rsym value: 0.183 / % possible all: 68.6 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: SAD / Resolution: 2.7→19.76 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 4936444 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 40.952 Å2 / ksol: 0.319 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.4 Å2
| ||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→19.76 Å
| ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.7→2.81 Å / Rfactor Rfree error: 0.049 / Total num. of bins used: 10
| ||||||||||||||||||||||||||||||||||||||||
Xplor file |
|