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Yorodumi- PDB-2f40: Structure of a Novel Protein from Backbone-Centered NMR Data and ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2f40 | ||||||
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Title | Structure of a Novel Protein from Backbone-Centered NMR Data and NMR-Assisted Structure Prediction | ||||||
Components | hypothetical protein PF1455Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / protein structure prediction / residual dipolar couplings / Pyrococcus furious / simulated annealing / PSI / Protein Structure Initiative / Southeast Collaboratory for Structural Genomics / SECSG | ||||||
Function / homology | Uncharacterised protein PF11491 family, DUF3213 / Protein of unknown function DUF3213 / Protein of unknown function (DUF3213) / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta / : / Uncharacterized protein Function and homology information | ||||||
Biological species | Pyrococcus furiosus (archaea) | ||||||
Method | SOLUTION NMR / RDC directed fragment assembly | ||||||
Authors | Bansal, S. / Prestegard, J.H. / Southeast Collaboratory for Structural Genomics (SECSG) | ||||||
Citation | Journal: Proteins / Year: 2006 Title: Structure determination of a new protein from backbone-centered NMR data and NMR-assisted structure prediction. Authors: Mayer, K.L. / Qu, Y. / Bansal, S. / LeBlond, P.D. / Jenney, F.E. / Brereton, P.S. / Adams, M.W. / Xu, Y. / Prestegard, J.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2f40.cif.gz | 246.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2f40.ent.gz | 211.5 KB | Display | PDB format |
PDBx/mmJSON format | 2f40.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f4/2f40 ftp://data.pdbj.org/pub/pdb/validation_reports/f4/2f40 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11245.694 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus furiosus (archaea) / Strain: DSM 3638 / Plasmid: pRIL / Production host: Escherichia coli (E. coli) / Strain (production host): TOP10, BL21(DE3) / References: GenBank: 18977827, UniProt: Q8U0X6*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: RDC directed fragment assembly / Software ordinal: 1 Details: limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformers submitted total number: 10 |