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Yorodumi- PDB-2e9g: Solution structure of the alpha adaptinC2 domain from human Adapt... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2e9g | ||||||
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Title | Solution structure of the alpha adaptinC2 domain from human Adapter-related protein complex 1 gamma 2 subunit | ||||||
Components | AP-1 complex subunit gamma-2AP-1 transcription factor | ||||||
Keywords | PROTEIN BINDING / beta-sandwich / immunoglobulin-like fold / adaptin / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information AP-1 adaptor complex / Golgi to vacuole transport / clathrin adaptor activity / Lysosome Vesicle Biogenesis / Golgi-associated vesicle / transport vesicle / vesicle-mediated transport / intracellular protein transport / endosome membrane / Golgi membrane ...AP-1 adaptor complex / Golgi to vacuole transport / clathrin adaptor activity / Lysosome Vesicle Biogenesis / Golgi-associated vesicle / transport vesicle / vesicle-mediated transport / intracellular protein transport / endosome membrane / Golgi membrane / Golgi apparatus / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, restrained molecular dynamics | ||||||
Authors | Tomizawa, T. / Koshiba, S. / Watanabe, S. / Harada, T. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the alpha adaptinC2 domain from human Adapter-related protein complex 1 gamma 2 subunit Authors: Tomizawa, T. / Koshiba, S. / Watanabe, S. / Harada, T. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2e9g.cif.gz | 789.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2e9g.ent.gz | 686.2 KB | Display | PDB format |
PDBx/mmJSON format | 2e9g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e9/2e9g ftp://data.pdbj.org/pub/pdb/validation_reports/e9/2e9g | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14372.376 Da / Num. of mol.: 1 / Fragment: Alpha adaptinC2 domain, GAE Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: AP1G2 / Plasmid: P060123-24 / References: UniProt: O75843 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.54mM alpha adaptinC2 domain U-15N,13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O, 90% H2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 296 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE II / Manufacturer: Bruker / Model: AVANCE II / Field strength: 900 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, restrained molecular dynamics Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |