+Open data
-Basic information
Entry | Database: PDB / ID: 1zcd | ||||||
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Title | Crystal structure of the Na+/H+ antiporter NhaA | ||||||
Components | Na(+)/H(+) antiporter 1 | ||||||
Keywords | MEMBRANE PROTEIN / antiporter | ||||||
Function / homology | Function and homology information response to alkaline pH / sodium:proton antiporter activity / cardiolipin binding / response to salt stress / regulation of intracellular pH / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.45 Å | ||||||
Authors | Hunte, C. / Screpanti, E. / Venturi, M. / Rimon, A. / Padan, E. / Michel, H. | ||||||
Citation | Journal: Nature / Year: 2005 Title: Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Authors: Hunte, C. / Screpanti, E. / Venturi, M. / Rimon, A. / Padan, E. / Michel, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zcd.cif.gz | 145.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zcd.ent.gz | 116.9 KB | Display | PDB format |
PDBx/mmJSON format | 1zcd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zc/1zcd ftp://data.pdbj.org/pub/pdb/validation_reports/zc/1zcd | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 41388.457 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: nhaA / Plasmid: pAXH / Production host: Escherichia coli (E. coli) / Strain (production host): RK20 / References: UniProt: P13738 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 4.95 Å3/Da / Density % sol: 75.13 % |
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Crystal grow | Temperature: 279 K / Method: vapor diffusion, hanging drop / pH: 4 Details: PEG 400, MgCl2, KCl, citrate, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
-Data collection
Diffraction |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 3.45→20 Å / Num. all: 21922 / Num. obs: 19993 / % possible obs: 91.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 18.4 % / Net I/σ(I): 30.5 | ||||||||||||||||||||
Reflection shell | Resolution: 3.45→3.57 Å / % possible all: 64.1 |
-Processing
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Refinement | Method to determine structure: SAD / Resolution: 3.45→15 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.45→15 Å
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Refine LS restraints |
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