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Yorodumi- PDB-1yub: SOLUTION STRUCTURE OF AN RRNA METHYLTRANSFERASE (ERMAM) THAT CONF... -
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-Basic information
Entry | Database: PDB / ID: 1yub | ||||||
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Title | SOLUTION STRUCTURE OF AN RRNA METHYLTRANSFERASE (ERMAM) THAT CONFERS MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN ANTIBIOTIC RESISTANCE, NMR, MINIMIZED AVERAGE STRUCTURE | ||||||
Components | RRNA METHYLTRANSFERASE | ||||||
Keywords | METHYLTRANSFERASE / ERM / ERMAM / MLS ANTIBIOTICS / RRNA | ||||||
Function / homology | Function and homology information 23S rRNA (adenine2085-N6)-dimethyltransferase / 23S rRNA (adenine(2085)-N(6))-dimethyltransferase activity / rRNA (adenine-N6,N6-)-dimethyltransferase activity / response to antibiotic / RNA binding Similarity search - Function | ||||||
Biological species | Streptococcus pneumoniae (bacteria) | ||||||
Method | SOLUTION NMR / DG, SA | ||||||
Authors | Yu, L. / Petros, A.M. / Schnuchel, A. / Zhong, P. / Severin, J.M. / Walter, K. / Holzman, T.F. / Fesik, S.W. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance. Authors: Yu, L. / Petros, A.M. / Schnuchel, A. / Zhong, P. / Severin, J.M. / Walter, K. / Holzman, T.F. / Fesik, S.W. #1: Journal: Nat.Struct.Biol. / Year: 1997 Title: Erratum. Solution Structure of an Rrna Methyltransferase (Ermam) that Confers Macrolide-Lincosamide-Streptogramin Antibiotic Resistance Authors: Yu, L. / Petros, A.M. / Schnuchel, A. / Zhong, P. / Severin, J.M. / Walter, K. / Holzman, T.F. / Fesik, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1yub.cif.gz | 98.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1yub.ent.gz | 82.6 KB | Display | PDB format |
PDBx/mmJSON format | 1yub.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yu/1yub ftp://data.pdbj.org/pub/pdb/validation_reports/yu/1yub | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 28862.568 Da / Num. of mol.: 1 / Mutation: I75T, S100N, H118R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae (bacteria) Strain: 5728, A CLINICAL ISOLATE FROM ABBOTT CULTURE COLLECTION Cell line: BL21 / Gene: ERM / Plasmid: PET-24(+) / Species (production host): Escherichia coli / Gene (production host): ERM / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P21236, EC: 2.1.1.48 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | Ionic strength: 50 mM SODIUM PHOSPHATE, 100 mM NACL / pH: 6.5 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: DG, SA / Software ordinal: 1 Details: THE TOTAL NUMBER OF DISTANCE RESTRAINTS USED WAS 3259. THE TOTAL NUMBER OF TORSIONAL RESTRAINTS USED WAS 63. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY AND LEAST RESTRAINT VIOLATION Conformers calculated total number: 17 / Conformers submitted total number: 1 |