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Yorodumi- PDB-1ygu: Crystal structure of the tandem phosphatase domains of RPTP CD45 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ygu | ||||||
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Title | Crystal structure of the tandem phosphatase domains of RPTP CD45 with a pTyr peptide | ||||||
Components |
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Keywords | HYDROLASE / CD45 / protein tyrosine phosphatase / phosphotyrosine / polyoma middle T antigen / RPTP | ||||||
Function / homology | Function and homology information plasma membrane raft distribution / positive regulation of antigen receptor-mediated signaling pathway / membrane microdomain / regulation of protein tyrosine kinase activity / positive regulation of protein tyrosine phosphatase activity / positive regulation of hematopoietic stem cell migration / negative regulation of cytokine-mediated signaling pathway / alpha-beta T cell proliferation / negative regulation of protein tyrosine kinase activity / positive regulation of Fc receptor mediated stimulatory signaling pathway ...plasma membrane raft distribution / positive regulation of antigen receptor-mediated signaling pathway / membrane microdomain / regulation of protein tyrosine kinase activity / positive regulation of protein tyrosine phosphatase activity / positive regulation of hematopoietic stem cell migration / negative regulation of cytokine-mediated signaling pathway / alpha-beta T cell proliferation / negative regulation of protein tyrosine kinase activity / positive regulation of Fc receptor mediated stimulatory signaling pathway / negative regulation of cell adhesion involved in substrate-bound cell migration / regulation of interleukin-8 production / negative regulation of microglial cell activation / Other semaphorin interactions / negative regulation of T cell mediated cytotoxicity / positive regulation of humoral immune response mediated by circulating immunoglobulin / DN2 thymocyte differentiation / cell cycle phase transition / negative regulation of protein autophosphorylation / gamma-delta T cell differentiation / natural killer cell differentiation / positive regulation of gamma-delta T cell differentiation / transmembrane receptor protein tyrosine phosphatase activity / : / bleb / positive regulation of alpha-beta T cell proliferation / positive regulation of isotype switching to IgG isotypes / negative thymic T cell selection / stem cell development / heparan sulfate proteoglycan binding / positive thymic T cell selection / regulation of phagocytosis / positive regulation of extrinsic apoptotic signaling pathway / heterotypic cell-cell adhesion / bone marrow development / regulation of receptor signaling pathway via JAK-STAT / negative regulation of interleukin-2 production / ankyrin binding / leukocyte cell-cell adhesion / spectrin binding / response to aldosterone / positive regulation of stem cell proliferation / Phosphorylation of CD3 and TCR zeta chains / B cell proliferation / : / positive regulation of immunoglobulin production / T cell differentiation / host cell membrane / positive regulation of protein kinase activity / hematopoietic progenitor cell differentiation / positive regulation of phagocytosis / dephosphorylation / positive regulation of B cell proliferation / extrinsic apoptotic signaling pathway / release of sequestered calcium ion into cytosol / positive regulation of T cell proliferation / positive regulation of interleukin-2 production / T cell activation / protein dephosphorylation / B cell differentiation / protein-tyrosine-phosphatase / secretory granule membrane / protein tyrosine phosphatase activity / response to gamma radiation / B cell receptor signaling pathway / negative regulation of protein kinase activity / cytoplasmic side of plasma membrane / negative regulation of ERK1 and ERK2 cascade / positive regulation of T cell mediated cytotoxicity / MAPK cascade / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of tumor necrosis factor production / heparin binding / T cell receptor signaling pathway / regulation of gene expression / defense response to virus / positive regulation of MAPK cascade / positive regulation of ERK1 and ERK2 cascade / cell surface receptor signaling pathway / regulation of cell cycle / membrane raft / external side of plasma membrane / signaling receptor binding / focal adhesion / Neutrophil degranulation / protein kinase binding / cell surface / extracellular exosome / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Nam, H. / Poy, F. / Saito, H. / Frederick, C.A. | ||||||
Citation | Journal: J.Exp.Med. / Year: 2005 Title: Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. Authors: Nam, H.J. / Poy, F. / Saito, H. / Frederick, C.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ygu.cif.gz | 245.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ygu.ent.gz | 196.4 KB | Display | PDB format |
PDBx/mmJSON format | 1ygu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yg/1ygu ftp://data.pdbj.org/pub/pdb/validation_reports/yg/1ygu | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | Two biological units are present in an asymmetric unit. |
-Components
#1: Protein | Mass: 71500.625 Da / Num. of mol.: 2 / Fragment: D1 and D2 PTP domains / Mutation: C828S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PTPRC, CD45 / Plasmid: pET / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P08575, protein-tyrosine-phosphatase #2: Protein/peptide | Mass: 546.464 Da / Num. of mol.: 2 / Fragment: Middle T antigen (residues 248-251, SWS:P03077) / Source method: obtained synthetically / Details: Polyoma Middle T antigen pTyr peptide / References: UniProt: P03077 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.3 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: PEG 4000, sodium acetate, glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.95 Å |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Sep 3, 2003 |
Radiation | Monochromator: channel cut crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→30 Å / Num. all: 34492 / Num. obs: 32025 / % possible obs: 92.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Biso Wilson estimate: 57.5 Å2 / Rsym value: 0.079 / Net I/σ(I): 17.9 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.273 / Mean I/σ(I) obs: 2.1 / Num. unique all: 2268 / % possible all: 67 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: D2 domain of RPTP LAR Resolution: 2.9→29.16 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1758427.37 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 22.2085 Å2 / ksol: 0.28125 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 78 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.9→29.16 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.08 Å / Rfactor Rfree error: 0.033 / Total num. of bins used: 6
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Xplor file |
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