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Yorodumi- PDB-1wlo: Solution structure of the hypothetical protein from thermus therm... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wlo | ||||||
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Title | Solution structure of the hypothetical protein from thermus thermophilus HB8 | ||||||
Components | sufE proteinShanghai University of Finance and Economics | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Fe-S metabolism associated domain, SufE-like / Fe-S metabolism associated domain / Sufe protein. Chain: A - #10 / Sufe protein. Chain: A / Alpha-Beta Complex / Alpha Beta / Iron-sulfur cluster biosynthesis protein SufE Function and homology information | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: to be published Title: Solution structure of the hypothetical protein from thermus thermophilus HB8 Authors: Hayashi, F. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wlo.cif.gz | 830.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wlo.ent.gz | 722.5 KB | Display | PDB format |
PDBx/mmJSON format | 1wlo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wl/1wlo ftp://data.pdbj.org/pub/pdb/validation_reports/wl/1wlo | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15179.624 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Plasmid: pET11a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q5SK87 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.72mM protein U-15N, 13C; 20mM phosphate buffer NA; 150mM NaCl; 0.02% NaN3; 1mM DTT Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 170mM / pH: 7.2 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |