+Open data
-Basic information
Entry | Database: PDB / ID: 1waj | ||||||
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Title | DNA POLYMERASE FROM BACTERIOPHAGE RB69 | ||||||
Components | DNA POLYMERASE | ||||||
Keywords | NUCLEOTIDYLTRANSFERASE / RB69 DNA POLYMERASE (GP43) | ||||||
Function / homology | Function and homology information bidirectional double-stranded viral DNA replication / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / 3'-5' exonuclease activity / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nucleotide binding / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Enterobacteria phage RB69 (virus) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.8 Å | ||||||
Authors | Wang, J. / Satter, A.K.M.A. / Wang, C.C. / Karam, J.D. / Konigsberg, W.H. / Steitz, T.A. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1997 Title: Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69. Authors: Wang, J. / Sattar, A.K. / Wang, C.C. / Karam, J.D. / Konigsberg, W.H. / Steitz, T.A. #1: Journal: To be Published Title: Structural Basis for Phenotypes of Mutant Pol Alpha Family DNA Polymerases Authors: Wang, J. / Steitz, T. #2: Journal: Biochemistry / Year: 1996 Title: Crystal Structures of an NH2-Terminal Fragment of T4 DNA Polymerase and its Complexes with Single-Stranded DNA and with Divalent Metal Ions Authors: Wang, J. / Yu, P. / Lin, T.C. / Konigsberg, W.H. / Steitz, T.A. #3: Journal: J.Biol.Chem. / Year: 1995 Title: Modular Organization of T4 DNA Polymerase. Evidence from Phylogenetics Authors: Wang, C.C. / Yeh, L.S. / Karam, J.D. #4: Journal: J.Biol.Chem. / Year: 1988 Title: Primary Structure of T4 DNA Polymerase. Evolutionary Relatedness to Eucaryotic and Other Procaryotic DNA Polymerases Authors: Spicer, E.K. / Rush, J. / Fung, C. / Reha-Krantz, L.J. / Karam, J.D. / Konigsberg, W.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1waj.cif.gz | 191.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1waj.ent.gz | 151.9 KB | Display | PDB format |
PDBx/mmJSON format | 1waj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wa/1waj ftp://data.pdbj.org/pub/pdb/validation_reports/wa/1waj | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 104743.156 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage RB69 (virus) / Genus: T4-like viruses / Gene: POL / Gene (production host): POL / Production host: Escherichia coli (E. coli) / References: UniProt: Q38087, DNA-directed DNA polymerase |
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#2: Chemical | ChemComp-5GP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.55 Å3/Da / Density % sol: 80 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.6 / Details: pH 5.6 | ||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 75 % | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Sep 1, 1994 / Details: MIRROR |
Radiation | Monochromator: MIRROR / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→40 Å / Num. obs: 42608 / % possible obs: 89 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.089 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.8→40 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.8→40 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |