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Yorodumi- PDB-1umx: PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ARG M267 REPLACED WITH... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1umx | ||||||
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Title | PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ARG M267 REPLACED WITH LEU (CHAIN M, R267L) | ||||||
Components | (REACTION CENTER PROTEIN ...Photosynthetic reaction centre) x 3 | ||||||
Keywords | PHOTOSYNTHESIS / PHOTOSYNTHETIC REACTION CENTER / TRANSMEMBRANE / ELECTRON TRANSPORT / CARDIOLIPIN / MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information : / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / membrane => GO:0016020 / metal ion binding Similarity search - Function | ||||||
Biological species | RHODOBACTER SPHAEROIDES (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Fyfe, P.K. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2004 Title: Disruption of a specific molecular interaction with a bound lipid affects the thermal stability of the purple bacterial reaction centre. Authors: Fyfe, P.K. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1999 Title: Structural Details of an Interaction between Cardiolipin and an Integral Membrane Protein Authors: Mcauley, K.E. / Fyfe, P.K. / Ridge, J.P. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. #2: Journal: Biochemistry / Year: 1998 Title: Structural Studies of Wild-Type and Mutant Reaction Centers from an Antenna-Deficient Strain of Rhodobacter Sphaeroides: Monitoring the Optical Properties of the Complex from Bacterial Cell to Crystal Authors: Mcauley-Hecht, K.E. / Fyfe, P.K. / Ridge, J.P. / Prince, S.M. / Hunter, C.N. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. #3: Journal: Biochemistry / Year: 1992 Title: Construction and Characterization of a Mutant of Rhodobacter Sphaeroides with the Reaction Center as the Sole Pigment-Protein Complex Authors: Jones, M.R. / Visschers, R.W. / Van Grondelle, R. / Hunter, C.N. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1umx.cif.gz | 183.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1umx.ent.gz | 154.4 KB | Display | PDB format |
PDBx/mmJSON format | 1umx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/um/1umx ftp://data.pdbj.org/pub/pdb/validation_reports/um/1umx | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-REACTION CENTER PROTEIN ... , 3 types, 3 molecules HLM
#1: Protein | Mass: 28066.322 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHODOBACTER SPHAEROIDES (bacteria) / Strain: RM267L / Plasmid: PRKEH10D / Production host: RHODOBACTER SPHAEROIDES (bacteria) / Strain (production host): AM260W / References: UniProt: P11846, UniProt: P0C0Y7*PLUS |
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#2: Protein | Mass: 31346.389 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHODOBACTER SPHAEROIDES (bacteria) / Strain: RM267L / Plasmid: PRKEH10D / Production host: RHODOBACTER SPHAEROIDES (bacteria) / Strain (production host): AM260W / References: UniProt: P02954, UniProt: P0C0Y8*PLUS |
#3: Protein | Mass: 34354.504 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHODOBACTER SPHAEROIDES (bacteria) / Strain: RM267L / Plasmid: PRKEH10D / Production host: RHODOBACTER SPHAEROIDES (bacteria) / Strain (production host): AM260W / References: UniProt: P02953, UniProt: P0C0Y9*PLUS |
-Non-polymers , 8 types, 28 molecules
#4: Chemical | #5: Chemical | ChemComp-BCL / #6: Chemical | #7: Chemical | ChemComp-FE / | #8: Chemical | ChemComp-SPN / | #9: Chemical | ChemComp-U10 / | #10: Chemical | ChemComp-PO4 / | #11: Water | ChemComp-HOH / | |
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-Details
Compound details | ENGINEERED MUTATION IN CHAIN M, ARG 267 LEU FUNCTION: THE REACTION CENTER IS A MEMBRANE-BOUND ...ENGINEERED |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.5 Å3/Da / Density % sol: 76 % |
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Crystal grow | pH: 8 / Details: pH 8.00 |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.5 / Wavelength: 0.95 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 15, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→29 Å / Num. obs: 50050 / % possible obs: 97.5 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.062 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.44 / % possible all: 92.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA) Resolution: 2.8→29 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.904 / SU B: 9.089 / SU ML: 0.185 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.361 / ESU R Free: 0.267 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→29 Å
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Refine LS restraints |
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