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Yorodumi- PDB-1t2k: Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1t2k | ||||||
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Title | Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA | ||||||
Components |
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Keywords | Transcription/DNA / PROTEIN DNA COMPLEX / transcription / Transcription-DNA COMPLEX | ||||||
Function / homology | Function and homology information abducens nucleus development / hypoglossal nucleus development / detection of cell density / H4 histone acetyltransferase complex / growth plate cartilage chondrocyte proliferation / facial nucleus development / growth plate cartilage chondrocyte differentiation / positive regulation of cardiac muscle myoblast proliferation / cellular response to anisomycin / positive regulation of transforming growth factor beta2 production ...abducens nucleus development / hypoglossal nucleus development / detection of cell density / H4 histone acetyltransferase complex / growth plate cartilage chondrocyte proliferation / facial nucleus development / growth plate cartilage chondrocyte differentiation / positive regulation of cardiac muscle myoblast proliferation / cellular response to anisomycin / positive regulation of transforming growth factor beta2 production / cAMP response element binding / transcription factor AP-1 complex / cellular lipid metabolic process / leucine zipper domain binding / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / cAMP response element binding protein binding / IRF3 mediated activation of type 1 IFN / MDA-5 signaling pathway / release from viral latency / histone H2B acetyltransferase activity / macrophage apoptotic process / positive regulation of mitochondrial membrane permeability involved in apoptotic process / cellular response to leucine starvation / brainstem development / programmed necrotic cell death / histone H4 acetyltransferase activity / WNT5:FZD7-mediated leishmania damping / NK T cell differentiation / vacuole organization / neurofilament cytoskeleton organization / TRIF-dependent toll-like receptor signaling pathway / apoptotic process involved in development / NGF-stimulated transcription / SMAD protein signal transduction / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / IRF3-mediated induction of type I IFN / positive regulation of type I interferon-mediated signaling pathway / positive regulation by host of viral transcription / intrinsic apoptotic signaling pathway in response to hypoxia / mitotic intra-S DNA damage checkpoint signaling / mRNA transcription / motor neuron apoptotic process / toll-like receptor 4 signaling pathway / response to osmotic stress / TRAF6 mediated IRF7 activation / DNA-binding transcription activator activity / immune system process / nuclear chromosome / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / cellular response to exogenous dsRNA / cytoplasmic pattern recognition receptor signaling pathway / type I interferon-mediated signaling pathway / hepatocyte apoptotic process / negative regulation of DNA binding / outflow tract morphogenesis / Activation of the AP-1 family of transcription factors / ubiquitin-like protein ligase binding / p38MAPK cascade / positive regulation of interferon-alpha production / antiviral innate immune response / R-SMAD binding / Response of EIF2AK4 (GCN2) to amino acid deficiency / general transcription initiation factor binding / white fat cell differentiation / positive regulation of type I interferon production / negative regulation by host of viral transcription / BMP signaling pathway / adipose tissue development / cis-regulatory region sequence-specific DNA binding / hematopoietic progenitor cell differentiation / histone acetyltransferase activity / JNK cascade / lipopolysaccharide-mediated signaling pathway / transcription initiation-coupled chromatin remodeling / positive regulation of vascular associated smooth muscle cell proliferation / cellular response to cadmium ion / TICAM1-dependent activation of IRF3/IRF7 / Regulation of innate immune responses to cytosolic DNA / positive regulation of interferon-beta production / response to endoplasmic reticulum stress / GTPase activator activity / negative regulation of angiogenesis / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / transforming growth factor beta receptor signaling pathway / liver development / Regulation of PTEN gene transcription / Negative regulators of DDX58/IFIH1 signaling / promoter-specific chromatin binding / TP53 Regulates Transcription of DNA Repair Genes / FCERI mediated MAPK activation / RNA polymerase II transcription regulatory region sequence-specific DNA binding / peptidyl-threonine phosphorylation / MAPK6/MAPK4 signaling / DDX58/IFIH1-mediated induction of interferon-alpha/beta / euchromatin / Heme signaling / mRNA transcription by RNA polymerase II / Transcriptional activation of mitochondrial biogenesis / response to organic cyclic compound Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Panne, D. / Maniatis, T. / Harrison, S.C. | ||||||
Citation | Journal: Embo J. / Year: 2004 Title: Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer. Authors: Panne, D. / Maniatis, T. / Harrison, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t2k.cif.gz | 117 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t2k.ent.gz | 86 KB | Display | PDB format |
PDBx/mmJSON format | 1t2k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t2/1t2k ftp://data.pdbj.org/pub/pdb/validation_reports/t2/1t2k | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-DNA chain , 2 types, 2 molecules EF
#1: DNA chain | Mass: 9724.338 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: interferon-b enhancer |
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#2: DNA chain | Mass: 9336.002 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: interferon-b enhancer |
-Protein , 3 types, 4 molecules ABCD
#3: Protein | Mass: 13058.792 Da / Num. of mol.: 2 / Fragment: N-terminal DNA binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IRF3 / Plasmid: pTXB3 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q14653 #4: Protein | | Mass: 7314.744 Da / Num. of mol.: 1 / Fragment: bZip domain / Mutation: C269S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: JUN / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P05412 #5: Protein | | Mass: 7133.292 Da / Num. of mol.: 1 / Fragment: bZip domain / Mutation: C351S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ATF2, CREB2, CREBP1 / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P15336 |
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-Non-polymers , 1 types, 8 molecules
#6: Water | ChemComp-HOH / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.5 Å3/Da / Density % sol: 73.4 % | ||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 100 mM NaCacodylate, pH 6.5, 12.5% (w/v) PEG 6000, 400 mM NH4OAc, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.9 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 23, 2001 Details: Bent triangular asymmetric cut Si(111) monochromater; Rh-coated Si mirror for vertical focussing |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 20151 / Observed criterion σ(F): 4 / Observed criterion σ(I): 2 / Biso Wilson estimate: 36.5 Å2 / Rsym value: 0.075 / Net I/σ(I): 19.4 |
Reflection shell | Resolution: 3→3.19 Å / Mean I/σ(I) obs: 5.7 / Rsym value: 0.413 / % possible all: 94 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2IRF and 2DGC Resolution: 3→29.98 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 2048126.71 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 27.1663 Å2 / ksol: 0.280152 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 88 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→29.98 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.19 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 6
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Xplor file |
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